Browse Our Biology and Life Sciences Journals
Biology is a study of living objects and their life processes. It examines all aspects of living organisms such as their occurrence, classification, internal and external structure, nutrition, reproduction, inheritance, etc. The term “biology” is commonly replaced by the terms “life sciences” and “biological sciences.” There are dozens of branches of biology. Some of the major ones include:
Biology and Life Sciences
40 accessions of Aegilops tauschii Coss. specially chosen to present as much of the species variability as possible in such sample were used to build a phylogenetic tree based on transcriptomes sequences. The tree displays subdivision of Ae. tauschii into TauL1, TauL2 and TauL3 gene-pools, and also into subsp. tauschii (TauL1) and subsp. strangulata (TauL2 + TauL3). TauL3 does not look as an outlier. Both subsp. strangulata and subsp. tauschii are highly variable. Accessions from all clades of subsp. tauschii and some clades of subsp. strangulata have disjoint patterns of geographic occurrence, while some of subsp. strangulata clades correspond to particular geographical regions. No special separate clade was formed by some accessions of Ae. tauschii subsp. tauschii from Yellow River region.
Abstract
Thirty nine species of oribatid mites (Oribatida), belonging to 29 genera and 20 families were collected from semidecayed leaves in the San Gabriel Limestone Cave, Dominican Republic. A new species of the genus Pergalumna (Galumnidae)—P. (Pergalumna) sangabrielensis n. sp.—is described.
A comprehensive lichenological survey was conducted on three Azorean islands: Faial, São Miguel, and Terceira, resulting in the documentation of 155 lichens and lichenicolous fungi species, with a total of 406 observations. Among these, 33 species are reported for the first time in the Archipelago, with four species representing new records for Europe (Lecanora subimmersa, Lecanora thysanota, Pseudosagedia cestrensis, and Traponora asterella). Additionally, 15 species and varieties are new to Portugal, including notable taxa such as Caloplaca interna, Lecanora gisleriana, Lecanora handelii, Variospora dolomiticola, and Xanthocarpia diffusa.
Salsola is one of the largest genera in the tribe Salsoleae within Amaranthaceae, with many species that share morphological characteristics, which makes it a challenging genus to study. In this study, 11 quantitative and eight qualitative morphological traits were evaluated and measured. Ward’s dendrogram showed two main clusters: one composed of S. brachiata and S. turcomanica, and the other composed of two subclusters, with populations of S. kali, S. incanescens, S. orientalis and S. dendroides. In addition, we performed molecular phylogenetic analyses conducted with sequence data from nrDNA (ITS and ETS) and cpDNA markers (psbB-psbH and atpB-rbcL) under maximum parsimony, Bayesian inference and maximum likelihood approaches. All the members were clustered into a well-supported clade (PP = 1, ML/BS = 0.89) composed of two subclades: subclade A included S. turcomanica and S. brachiata (PP = 0.90, ML/BS = 73) and subclade B (PP = 0.95, ML/BS = 81) comprised S. incanescens, S. orientalis, S. dendroides and S. kali. The current study provides novel insights at morphological and molecular levels, as well as the results of molecular studies based on nr- and cpDNA sequence data that are congruent with morphological analyses.
Two species new to science, Xanthoria pedersenii and X. wennergrenii in the Xanthoria calcicola complex (Xanthoria, Xanthorioideae, Teloschistaceae), characterized by a complex of morphological, anatomical and molecular characters, are described, illustrated and compared with closely related taxa.
New Records for the Bryoflora of Vietnam, 6.
Bazzania tranninhiana sp. nov. (Lepidoziaceae)
During our visit to the Tam Đảo National Park in 1998, with the guidance of Prof. Trần Ninh, we collected a peculiar Bazzania species unknown from Vietnam before. This species, with its incurved, fragile leaf apices and deeply dissected underleaves proved to be new to science. This paper provides its illustrated description.
Peltula shalae Kumar S. is described as a new species of lichens from the Chenab Valley of Jammu and Kashmir, India. It is characterised by peltate-umbilicate thallus comprising of overlapping squamules forming rosettes, with diameter 2–3.5 cm. The absence of soredia is notable. This lichen is observed to grow on ‘shale rock’, a type of sedimentary rock, and is distinguished from closely related species, including P. daurica, P. sonorensis, P. anthracina, and P. obscurans by its distinct morphological features. Furthermore, a comprehensive comparison with closely related peltate-umbilicate species is presented.
Plasmodium falciparum is primarily transmitted by Anopheles gambiae. Malaria caused by Plasmodium falciparum is a major public health issue in western Kenya and sub-Saharan Africa, accounting for 90% of malaria deaths. The primary methods of malaria prevention are indoor residual spraying and the use of insecticide-treated nets. These tools face challenges such as mosquito resistance to insecticides as well as their toxic effect to the non-target organism, therefore this study aims to explore the application of lichen secondary metabolites as potential oral biological insecticides by assessing mosquito mortality in varying concentrations. Lichen secondary metabolites were extracted from Cladonia foliacea thalli. Bioassay experiments were conducted on A. gambiae Kisumu strain mosquitoes. Mortality rates were measured after ingesting sugar bait and lichen extracts in different concentrations. Three test replicates and negative control were used, with mortality measured after 4, 24, 48, and 72 hours. Analysis using three-way analysis of variance with twoway interactions was performed using R program to determine the effect of different lichen extract concentrations, time of exposures and mosquito sex on mortality. Our results showed that the ingestion of C. foliacea extract at 50 mg/ml and a post-exposure period of 24 to 48 hours had a maximum effect on the mortality rate of targeted male and female A. gambiae. No statistical difference was found between male and female mosquitoes in mortality. Our study confirms firstly that the extract of C. foliacea is a promising oral toxic agent against adult malaria vector A. gambiae.
Moroccan oases host a rich array of date palm varieties, renowned for the traditional expertise in crafting local products from dates. Date juice, a quintessential local product with significant heritage value, is facing a decline in appreciation. This study delves into indigenous knowledge surrounding date juice processing. Surveys involving one hundred women from the Alnif and Tata oases in Morocco’s pre-Saharan regions were conducted. Results reveal the use of 56 medicinal and aromatic plants from 33 families and 53 genera in date juice preparation, with Lamiaceae and Asteraceae families most prevalent. Noteworthy species include Corrigiola telephiifolia Pourr. (85 citations), Cynara humilis L. (66), Cyperus longus L. (65), and Alpinia officinarum Hance (64). Date juice holds significance in traditional medicine, prized for its sensory qualities and therapeutic benefits resulting from blending date fruit with aromatic and medicinal plants. The study sheds light on traditional manufacturing processes, including palm variety selection and plant choices. It also offers insights into therapeutic applications aligning with medicinal practices. These findings enrich understanding of cultural traditions and provide essential information for their preservation and propagation.
Ecological restoration requires large-scale reintroductions of plants, but their genetic basis is a controversial issue. Formerly, non-local seed sourcing of naturally occurring herbaceous species was common practice. Here we test whether the genetic pattern of the earlier introduction of non-local seeds of Leucanthemum vulgare agg. (ox-eye daisy) can still be detected several years after the application and whether it differs from that of the regional gene pool. We collected leaf material of the ox-eye daisy in Central Germany on sites of indigenous populations (I) and those formerly restored with non-local seed sources (R). Genome sizes and population genetic pattern (AFLP) were analysed. Genome size estimates of most of the individuals studied suggest, that most ox-eye daisies in the region have similar genome sizes regardless of their origin, while individuals from two indigenous populations from the most northwestern part of the study area had lower 1C values. All populations were genetically diverse and the former use of non-local geno-types of the species could not be detected up to more than 8 years after the establishment of the populations. The results shows that a recommendation for restoration purposes is unequivocal, it can only be concluded that it will be best to use seeds that are local and/or similar to the sites intended for sowing.
The new species Verrucaria aptrootii sp. nov. is described from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the position of this new species within the genus Verrucaria. Verrucaria aptrootii sp. nov. is closely related to V. squamulosa and characterized by a not squamulose thallus, plane to concave areoles, excipulum of 25–40 µm, simple, branched periphyses, ascospores of 27–35 × 10–15 µm and calcareous rock substrate.
Abstract
Introduction
This study was conducted to determine the specific features of the mutual influence of the immune and haemostatic systems in immunohaemostasis, the role of immune reactions in the regulation of blood coagulation, and the efficacy of modern methods of treating thrombosis and bleeding.
Methods
The study analysed relevant scientific sources on immunology and haematology and identified the specific features of the blood clotting process and the role of immune reactions in it.
Results
The study found that the immune system influences the haematological system through the interaction of blood clotting factors, platelets, plasminogen, endothelial cells with immune cells. The haemostatic system influences the immune system through mechanisms to maintain immune tolerance and immune memory and the properties of clotting factors to activate the stimulation and migration of immune cells to the site of infection. Immune reactions regulate blood coagulation by activating platelets, regulating blood coagulation factors, affecting fibrinolysis, and immune tolerance. The process of platelet activation involves immune cells, immune complexes, and microbial components. The regulation of blood coagulation factors is influenced by the ability of immune cells to produce activators and inhibitors of these factors and to stimulate or slow down fibrinolysis. The immune system's maintenance of immune tolerance to blood components is regulated by mechanisms of immune response suppression, partial immune ignoring of certain blood elements, inhibition of activation of certain immune cells, apoptosis, and selection of immature T-lymphocytes. Treatment methods for patients at risk of thrombosis and bleeding include anticoagulation, antiplatelet, dual antiplatelet therapy, thrombectomy, endovascular methods, medical prophylaxis of bleeding, and coagulation monitoring.
Conclusions
The findings of this study suggest the significance of immune responses in the regulation of blood coagulation processes, and therefore they can be used in the development of immunotherapy methods for the treatment of thrombosis and bleeding.
Abstract
Using efficient and safe preservation technologies to extend the shelf life of fruits and vegetables is always an important research topic. Among numerous preservation technologies, ultrasound is one of the most available and pollution-free methods that improve the storage quality of fruits and vegetables. The paper summarises the application of ultrasound and its combined treatment in fruit and vegetable storage in recent years, including the cleaning effect, passivation of enzymes, sterilisation, and the impact on quality indicators. In addition, the limitations of ultrasound technology in fruit and vegetable preservation are also detailed. Only under the appropriate conditions can ultrasound treatment extend the shelf life of fruits and vegetables. However, ultrasound combined with other technologies usually can get better preservation effects, providing a broader application prospect for ultrasound technology.
Abstract
Bio-waste (51.54% of total weight) was obtained from the head and shell of giant red shrimp (Aristaeomorpha foliacea), and comparison with the fatty acid profiles of shrimp meat revealed a higher total polyunsaturated fatty acids content in bio-waste. The study then investigated the effect of six solvents of different polarities (hexane, acetone, chloroform, methanol, ethanol, and isopropyl alcohol) on astaxanthin yield, antioxidant activity, and colour a* values (redness) of the bio-waste using ultrasound-assisted extraction (UAE). As a result, hexane showed the highest astaxanthin yield with a linear correlation observed between astaxanthin yield, antioxidant activity, and colour a* values. In contrast, methanol, ethanol, and acetone exhibited the lowest quality values. These findings underline the effectiveness of low polarity solvents in achieving maximum astaxanthin yield.
Abstract
Barbershops are important venues for men to get their hair and beards done and hence, play an important role in men's social life. But barbershops can also be a source of infections. The barber's profession brings the barber into direct contact with customers who may carry pathogens, and contaminated instruments or skin-to-skin contacts might transmit infectious agents. Since barbers work with non-sterile and reusable sharp objects, a simple nick might facilitate blood-borne infections. In our review article we summarize current knowledge regarding barbershop-acquired infections including transmission routes and preventive measures. In fact, shaving in barbershops, particularly when reusing non-disinfected razor blades increases the transmission risk for the human immunodeficiency virus (HIV) and hepatitis B or C viruses. Furthermore, distinct bacteria like Staphylococcus aureus, fungi (in particular dermatophytic Trichophyton species) as well as ectoparasitic lice could be identified upon screening of the barbers' equipment and working places. However, knowledge regarding and compliance in hygiene practices varied considerably among barbers. Notably, since in certain countries barbershops are venues to contact sex workers, sexually transmitted diseases might also be acquired in barbershops. In conclusion, improving hygiene standards including disinfection of reusable equipment, and surveillance of the preventive measures would reduce the risk for barbershop-acquired infections.
Abstract
Nowadays, drone imagery is a common way to quickly obtain information on the state of vegetation, as well as a method for taking orthophotos and terrain models. For terrain modelling, aerial photographs are required to have at least 60% overlap between adjacent images. Typically, the inadequate overlap is only discovered later, during post-processing. In our previous work we have presented a method we developed to determine on the spot, whether the in-flight images are suitable for producing the terrain model or whether it is necessary to re-render a part of the area, which can be done under the same conditions (weather, ionosphere, satellite geometry). Our proposed method for calculating the overlap is different from the usual procedure. Using VBA-based calculations, we computed the overlap between the images based on the position of the centre of the images, the altitude of the flight and the rotation angle of the images. The method was tested in practice, but we felt it necessary to verify our calculations. During the verification, we checked the VBA-based calculations using a Python script. The test showed that the results obtained with a previous midpoint calculation method were 92.2 percent identical to the calculations performed with Python. The Python program is accurate and fast; therefore, the use is recommended on the field.
Abstract
The rice bran waxes have a very interesting alcoholic moiety, a mixture of long-chain aliphatic fatty alcohols, collectively known as policosanol. This study aimed to investigate rice bran policosanol extract's effect on testosterone secretion in vitro and in vivo and explore the primary composition ratio. The proliferation of cells and the levels of testosterone in cultured rat Leydig cells were examined, while male sexual behaviour and serum testosterone levels were detected in hydrocortisone-induced mice. It was found that testosterone secretion in primary Leydig cells could be significantly stimulated by a policosanol extract featuring an octacosanol/triacontanol ratio of 3–5/1, with a ratio of 5/1 being the most optimal. A policosanol extract containing 68.72% octacosanol and 13.65% triacontanol was capable of reversing hydrocortisone-induced Kidney-Yang Deficiency syndrome by enhancing sexual behaviour and the weight of reproductive organs, as well as increasing testosterone levels. Consequently, the rice bran policosanol extract with an octacosanol/triacontanol ratio of 3–5/1 could stimulate the secretion of testosterone.
Abstract
Actinomycetoma, a neglected tropical disease affecting the skin and soft tissues, is primarily caused by filamentous bacteria including Nocardia species. Here, we report a healthy 56-year-old man who has a one-year history of nodular lesions with seropurulent discharge on his right knee. Despite negative initial tissue culture, the sulfur granules that were partially acid-fast and Gram-positive branching filamentous rods were revealed in the tissue section. Repeated investigation identified the rare pathogen Nocardia wallacei, using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal ribonucleic acid (rRNA) gene sequencing. The patient was successfully treated with a six-month course of trimethoprim-sulfamethoxazole.
This report describes a rare case of actinomycetoma due to N. wallacei, highlighting the challenges in diagnosis and the importance of accurate pathogen identification for the successful management of infection. The current literature regarding the causative agent will also be discussed.
Abstract
In this study, changes in the odour of apple slices throughout the drying process were monitored and documented using electronic nose sensors, while moisture levels were measured and recorded using an electronic hygrometer. The initial and final moisture contents of apple slices were determined as 86.81 ± 0.05% and 4.92 ± 0.01%, respectively. During drying, apple slices were monitored with six odour sensors powered by an Arduino microprocessor. As the moisture value of the apple slices decreased during the drying process, the electronic nose data also decreased. In addition, the image changes in the apple slices according to the drying level were taught to the Teachable Machine. In this study, it was observed that the machine was able to detect the drying level of apples with 85%–100% accuracy. When 900 apple samples were used in the test phase, machine learning was able to predict the drying level of apples with 100% accuracy based on the confusion matrix data. PCA analysis highlighted different patterns in the sensor data during drying; PLS analysis showed that the sensor data can accurately predict the colour (L*, a*, b*), diameter, and thickness of apple slices with high correlation coefficients (rCV and rPre > 0.9).
Abstract
This study evaluated the impact of storage temperature on the antimicrobial activity of essential oil emulsions of Thymus vulgaris L. (TEOE) and Laurus nobilis (LEOE) at 0.6% against Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), and Listeria innocua (ATCC 33090) inoculated onto cooked chicken breast to simulate post-cooking contamination. Samples stored under: T3 (regular storage at 3 ± 0.5 °C), T8 (inadequate storage 8 ± 1 °C), and T3A (refrigerated storage with simulated temperature abuse at 25 ± 1 °C). Microbial analysis and sensory evaluations were carried out at 0, 24, 36, 72, and 120 h of storage. The GC-MS analysis showed that carvacrol 31.97% and eucalyptol 41.16% were the main components in TEO and LEO, respectively. Storage temperature influenced the antibacterial effect of EOE. TEOE exhibited greater effectiveness at T8 compared to T3, reducing microbial counts to 1 log CFU g−1. Conversely, LEOE demonstrated superior efficacy under T3 in most instances and received a higher acceptability score. Additionally, at T3A, both emulsions demonstrated comparable effectiveness against all bacterial strains, although a slight reduction in antimicrobial proprieties were noted after simulated temperature abuse at 24 h. Therefore, the optimal combination for preserving cooked chicken meat is TEOE under T8 or LEOE under T3; assuming post-cooking contamination.
Abstract
Prescottella (Rhodococcus) soli is a soil-dwelling organism not previously thought to be pathogenic in humans. We discuss the case of a 78-year-old male renal transplant recipient presenting with respiratory symptoms and multiple pulmonary nodules, found to be pulmonary malakoplakia secondary to infection with Prescottella (Rhodococcus) soli. Treatment was commenced with vancomycin, meropenem and azithromycin for an induction period of two weeks and continued with indefinite oral moxifloxacin and azithromycin with significant clinical improvement. Although rare, Prescottella species, including Prescottella soli, should be considered in the differential diagnosis of pulmonary nodules, particularly in immunocompromised patients. More data is required to inform optimal treatment.
Abstract
Lotus root is a popular vegetable known for its nutritional value and unique taste. However, it can be susceptible to heavy metal contamination, which has raised concerns about its safety for consumption. This study aimed to analyse the extent of heavy metal contamination in lotus roots and assess the potential health risks associated with their consumption. The results showed that all measured heavy metal concentrations were below the maximum allowable limits, with contamination indices for all metals across nine provinces remaining below 1.0, classifying the lotus roots as “safe” for consumption. Non-carcinogenic risk assessment using the THQ showed values below 1.0 for all heavy metals in both adults and children, indicating negligible health risks. The HI was calculated as 0.107 for adults and 0.126 for children, with arsenic contributing significantly to these values at 47.76% for adults and 39.55% for children. The carcinogenic risk assessment revealed Carcinogenic Risk (CR) values of 8.37 × 10−8 for Pb, 2.04 × 10−5 for Cr, and 2.30 × 10−5 for As in adults, and 9.86 × 10−8 for Pb, 2.40 × 10−5 for Cr, and 2.71 × 10−5 for As in children. All CR values were within the acceptable range, indicating no apparent carcinogenic effects from lotus root consumption at current intake levels. However, it was noted that increased consumption could elevate risks associated with As, with limits of 174 and 93 g for adults and children, respectively, to maintain safety.
Abstract
Gluten-free bread (GFB) consisting of amaranth flour, tigernut (chufa tuber) flour, apple powder, carrot powder, and soy protein isolate can have osteoprotective effects. Glucocorticoid-induced osteoporosis (GIO) was chosen as a model of experimental osteoporosis. Experimental studies were carried out on white male Wistar rats (n = 150): Group 1 – healthy animals receiving standard diet (StD); Group 2 – GIO rats receiving StD; Group 3 – GIO animals receiving zoledronic acid (ZA) and StD; Group 4 – GIO rats receiving ZA and GFB; Group 5 – GIO animals receiving GFB. We evaluated the general condition of animals and carried out morphological and biochemical studies. Destructive changes in the spatial structure of the bone tissue in GIO, observed in Group 2, led to the inability of the bone to withstand the functional load. At the same time, isolated treatment with ZA had virtually no effect on the final morphological picture. Including GFB in the diet of experimental animals both alone and with ZA had a noticeable protective effect on conditions triggering GIO.
Abstract
Similar to other regions, in East Central Europe, agriculture is the most vulnerable sector regarding the consequences of climate change through increasing variability in weather conditions, even on the short-term and local scales. Agricultural engineers make decisions; higher education has a crucial role in taking proper actions. Our survey aimed to get up-to-date information on the knowledge and attitude of graduating students completing agriculture-related studies at 6 universities located in Hungary, the Czech Republic, Poland, and Slovakia, with particular attention to weather projections, crop production, and soil protection issues. Analyses revealed adequate lexical knowledge of young engineers in each country. They are familiar with the agro-environmental actualities and future trends, issues to address at the right time, and the repertoire of tools and practices for taking efficient measures. A positive attitude toward proactive actions was proved. We report proof of a high level of preparedness resulting from the proper efficacy of agricultural higher education. The content of the curricula and the teaching methodologies meet well the receptiveness to learning in the V4 countries. Our findings confirm that frequent updating of the content of subjects as well as incorporating practical applications that promote active learning and engagement, are more critical today than ever, and it is possible to achieve a high level of preparedness for the challenges in the agricultural sector.
Abstract
This study analyzed the impact of storing Golden Delicious apples at 3 °C with a relative humidity of 85 ± 5% in two environments, namely a darkroom (D03) and a lightroom (L03), on the fruit quality. The research evaluated weight loss (Δm) and color metrics (a*, b*, L*, ΔE, C*, hue angle). The number of apples used for each storage was 12. Weight loss was calculated by measuring the changes in mass during storage. Color differences (ΔE), chroma (C*), and hue angle were determined based on the L*a*b* scale values. Apples stored at 3 °C in D03 experienced a minimal percentage of weight loss of 7.02%. The results indicated that compared to the D03 storage, the L03 storage had a more significant increase in Δm and color variation. The non-destructive method of measuring color characteristics facilitates the assessment of the storage conditions for Golden Delicious apples.
Abstract
Nutritional information on packaging is becoming increasingly important in the food industry. Currently, labels are seen not only on the back of the packaging but also on the front. As there are many versions of front-of-pack labels (FoPLs), the research aims to determine which helps consumers the most in making decisions about which food to include in a healthier lifestyle. Nutri-Score, Guideline Daily Amount (GDA) and Multiple Traffic Lights (MTL) FoPLs on cereals were compared using eye-tracking (ET) and choice-based conjoint analysis (CBCA). CBCA was used to assess consumer preferences and the labels and products were also ranked. Based on the results, GDA type FoPL proved to be the most useful based on conjoint analysis, ranking and the analysis of ET parameters. This label helped participants the most in choosing the product that best fits into a healthier lifestyle. The Nutri-Score label, which offers little information on a product's nutritional content, was not favourably received by the Hungarian sample, who preferred more detailed FoP labels.
Abstract
This study aimed to survey the current distribution of Toxoplasma gondii (T. gondii) seropositivity within the cat population in Budapest area. Therefore, blood samples of 123 cats aged 0.5–18 years were collected. The measurements were performed by the commercial ID Screen® Toxoplasmosis Indirect Multi-species ELISA kit. The results indicate an overall 31.7% of seropositivity, which was significantly increasing with age. A correlation was also detected between the outdoor lifestyle and T. gondii infection. A significantly higher proportion of cats living outdoors were seropositive (38.8%) compared to those living indoor (18.6%) (P = 0.022). Finally, our study indicates a lower T. gondii seropositivity rate in cats compared to previous studies from Hungary, as well as from other European regions.
A vízpótlás talajvízszintre gyakorolt hatásának értékelése egy délnyugat-magyarországi példán keresztül
Evaluation of the water supply’s impact on groundwater levels, with an example from South-West Hungary
Az Európát is érintő jelenlegi, napjainkban zajló éghajlatváltozás statisztikailag szignifikáns felmelegedési tendenciával jellemezhető. Az éghajlatváltozás hatása a csapadékviszonyok és az evapotranszspirációs folyamatok megváltoztatásán keresztül érinti a vízkörforgalmat, így a csapadék eloszlásának és mennyiségének várható változásai a folyamatosan emelkedő hőmérséklettel együtt a növények nagyobb vízfelhasználását eredményezhetik, ami változásokat indukálhat a talajnedvességben, a talajvízben és ennek következtében a vízmérlegben. Az emelkedő hőmérséklet hatására a jövőben a növekvő transzspirációs igény valószínűleg a növényi közösségek fokozott talajvízfelvételét eredményezi, ami a talajvízszint további csökkenését is magával vonhatja. Ha ez bekövetkezik, a talajvízfüggő erdei közösségek regenerálódása ezeken a területeken kérdésessé válik, mivel a fiatal fák gyökérzete nem fogja tudni elérni a vízforrást. Következésképpen a Kárpát-medence sík vidékein és vizes élőhelyein a nagy vízigényű erdők fennmaradása kérdésessé válhat. A fás vegetáció számára kiemelten fontos a talajvíz szerepe a vegetációs időszakban, éppen ezért a talajvízszint változását régóta, rendszeresen monitorozzák.
Konkrét megoldás lehet az árhullámok visszaduzzasztásából származó vízpótlás. A pozitív vízpótlási beavatkozásokra példa a Kaszó LIFE projekt. A projekt célja a leromlott élőhelyek rehabilitációja volt, ami a kedvező ökológiai állapot stabilizálásával teszi lehetővé a hosszú távú megőrzést. A megvalósítás eszközeként tórehabilitációt és folyó-mederbordákat alkalmaztak, biztosítva a Szentai-erdő (Nyugat-belső-somogyi kistérség) erdeinek, kis lápjainak és gyepterületeinek vízellátás javítását.
Jelen kutatás célja pedig a Kaszó LIFE projekt vízpótlási beavatkozásai után, azok felszín alatti vízszintre gyakorolt hidrológiai hatásainak elemzése volt. A meteorológiai adatok és a monitoring adatok alapján elmondható, hogy a Szentai-erdőben 30 éves szárazodási tendencia volt tapasztalható, amelynek elsődleges mutatója a talajvízszint csökkenése. Kiemelendőek a 2016–2017-es vizsgálati időszak évei, amikor az alapvetően lefolyástalan vizsgálati területen nem volt elegendő csapadék a talajvíz szintjének fenntartásához. Kutatási eredményeink azonban azt mutatták, hogy a vízpótlást célzó beavatkozások (mederbordák és tározótavak) összességében a talajvízszint emelkedését biztosítják.
A két beavatkozási típus talajvízszint emelkedésére gyakorolt hatását vizsgálva arra a következtetésre jutottunk, hogy míg a tavak építése jelentősen befolyásolja a környező kutak vízszintjét, addig a mederbordák esetében kisebb mértékű a hatás.
This study explored the effects of salicylic acid on the growth and biochemical responses of peppermint (Mentha piperita L.) under different soil salinity levels, a major challenge in agricultural productivity. The experiment was conducted using a factorial design with salicylic acid applied at concentrations of 0, 5, 10, and 50 ppm, combined with sodium chloride-induced salinity at 0, 1,000, 2,000, and 3,000 mg kg–1 soil. Results demonstrated that increasing soil salinity negatively impacted plant growth, reducing plant height, leaf number, and the dry weight of aerial parts. Salinity stress also led to reduced nutrient uptake, particularly for essential elements like calcium and potassium, while increasing sodium levels in plant tissues. However, the foliar application of salicylic acid, especially at 50 ppm, significantly improved growth parameters and mitigated the detrimental effects of salinity. Notably, at the highest salinity level (3,000 mg kg–1), salicylic acid enhanced plant height by 11.1% and leaf number by 30.9% compared to untreated plants. Salicylic acid also boosted biochemical responses, such as increasing total phenolic and flavonoid content, which are critical for stress tolerance. This study underscores the potential of salicylic acid as a stress mitigator, promoting better growth and physiological resilience in peppermint under saline conditions, offering valuable insights for improving crop performance in salinity-affected regions.
Abstract
Background
The study assessed replicative human immunodeficiency virus-(HIV-) infection and replicative co-infections as well as molecular determinants of reduced susceptibility towards anti-retroviral therapy in a Ghanaian population of known HIV patients and a control group.
Methods
Real-time PCRs for HIV-1, HIV-2, hepatitis B virus (HBV) and hepatitis C virus (HCV) were run with serum samples from known Ghanaian HIV-patients (n = 975) and control individuals (n = 105). For 108 individuals, HIV-sequence analysis was performed.
Results
Prevalence of replicative HIV-1 infection was 59.8% (583/975) in the known HIV-positive population and 2.9% (3/105) in the controls. Prevalences of replicative HBV-infection were comparable with 3.4% (33/975) in the HIV-positive individuals and 3.8% (4/105) in the controls. HIV-2 and HCV sequences were not recorded. Almost perfect concordance between two compared HIV-1-PCR assays was indicated by Fleiss' Kappa >0.8. Sanger sequencing indicated CRF_02AG, G and A3 as the quantitatively dominating HIV-1 subtypes, a minority of 3.4% CXCR4 tropism and high detection rates of mutations mediating reduced susceptibility towards nucleoside reverse transcriptase inhibitors (71.9%, 64/89), non-nucleoside reverse transcriptase inhibitors (95.5%, 85/89), protease inhibitors (95.9%, 93/97) and integrase inhibitors (22.4%, 22/98).
Conclusions
The assessment did not suggest HIV-triggered increased replication of HBV and HCV in the investigated Ghanaian population.
Abstract
The number of human infections with multidrug-resistant (MDR) bacteria is increasing worldwide and constitutes a serious threat to human health. Given the lack of novel antibiotic compounds worsening this dilemma, alternative antibiotic-independent treatment and prevention strategies of infectious diseases applying natural compounds appear highly appreciable. Given the long-known health-beneficial and disease-alleviating properties of Cannabis, we performed a literature search summarizing current knowledge regarding the antibacterial effects of extracts from different parts of the Cannabis sativa plant and of defined Cannabis-derived molecules and their potential mode of action. The included studies revealed that various extracts and essential oils of C. sativa as well as major cannabinoids exerted potent activities against a broad spectrum of Gram-positive bacteria and against some Gram-negative bacterial species including MDR strains. Particularly the disruption of the bacterial cytoplasmic membrane by some cannabinoids resulted in potent antibacterial effects against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus. Furthermore, defined cannabinoids inhibited the formation of and eradicated existing bacterial biofilms. In conclusion, given their antibacterial properties distinct Cannabis-derived molecules expand the repertoire of antibiotics-independent treatment options in the combat of bacterial infectious diseases which should be further addressed in future studies including clinical trials.
Abstract
Background
The exploratory study assessed trends in the abundance of CTX-M-type extended spectrum beta-lactamase (ESBL) and vancomycin-resistance genes vanA and vanB in the stool samples of German soldiers and police officers returning from predominantly tropical deployments next to the common diarrheagenic Escherichia (E.) coli pathovars enteropathogenic Escherichia coli (EPEC), enterotoxigenic E. coli (ETEC) and enteroaggregative E. coli (EAEC)) as well as rarely imported Vibrio spp. between 2006 and 2024.
Methods
Surveillance was performed applying real-time polymerase chain reaction and results were stratified by World Health Organization region of deployment as well as by deployment period. For the latter, the study interval was divided into three pre-COVID-19-pandemic periods, the COVID-19-pandemic period and the post-COVID-19-pandemic period. Averaged prevalences were used as references.
Results
In stool samples of 1817 deployed German soldiers and 117 police officers, averaged prevalences were 47.9% and 24.8% for the ESBL-type beta-lactamase bla CTX-M, 30.2% and 14.5% for vanB, 9.0% and 17.9% for EPEC, 3.4% and 12.8% for ETEC, 4.0% and 3.4% for EAEC as well as 2.0% and 3.4% for Vibrio spp., respectively. While resistance genes peaked during early deployments, maximum prevalences for enteropathogens were seen later.
Conclusions
The assessment suggested time- and region-dependence of the assessed parameters.
Abstract
Milk kefir is gaining popularity due to its high probiotic content. This study focuses on fortifying milk kefir with date syrup to enhance its sensory attributes, with the goal of encouraging consumption among the younger generation. Date syrup was added to milk kefir in specific proportions (2, 4, 6, 8, and 10% per 100 mL of milk). The selection of the most suitable percentage (10%, 74 °Bx) was determined based on sensory preferences indicated by the panellists. The newly developed fermented beverage underwent physicochemical and biochemical analyses over a 14-day fermentation period. Results revealed that the addition of date syrup led to a significant (P ≤ 0.001) decrease in pH and total soluble solids (TSS) content, accompanied by a noteworthy increase in total phenolic, flavonoids, condensed tannins contents and antioxidant activity (almost 2-fold). Liquid chromatography - heated electrospray ionisation - mass spectrometry (LC-HESI-MS/MS) results identified the presence of a newly formed and important antifungal compound, p-hydroxyphenyllactic acid (HPLA), showing a progressive increase in quantity during the fermentation process (13.8-fold on the 14th day of fermentation). Hence, the outcomes of this study offer compelling evidence that a novel category of functional beverage can be developed by employing milk kefir as an appropriate starter with the incorporation of date syrup.
Abstract
The demand for gluten-free food products is expected to grow in the coming years. Quinoa's versatility allows it to replace traditional grains and adds high nutritional value to grain blends and processed food items. The present study was designed to prepare and evaluate gluten-free cookies by substituting rice with quinoa and keeping pearl and foxtail millet constant. A significant increase in protein (8.0–13.2 g/100 g), crude fibre (2.9–6.2%), ash (0.5–1.7%), and hardness (928.9–1736.9 g) was observed with increasing quinoa supplementation. A positive correlation of crude fibre with protein, hardness, and fracturability was observed at P ≤ 5%. Organoleptic evaluation indicated that cookies with 30% quinoa were overall accepted by the consumer. Also, these cookies had lower acid and peroxide values, thus, had longer shelf-lives. This is the first report for the use of quinoa with the mixture of rice and millet, which could be a decent alternative ingredient for gluten-free cookies formulations.
Abstract
Kudzu root (Pueraria lobata), known for its dual role as a medicinal herb and food ingredient, has proven anti-inflammatory and antioxidant properties. This study explored the effects of P. lobata extract (PLE) on D-galactose/lipopolysaccharide (D-GalN/LPS)-induced liver inflammation and oxidative stress in mice, along with its impact on gut microbiota. The in vivo studies indicated that PLE significantly reduced hepatic levels of pro-inflammatory cytokines (tumour necrosis factor-α, interleukin-1β, and interleukin-6) and increased the anti-inflammatory cytokine interleukin-10. It also lowered serum aspartate aminotransferase and alanine aminotransferase activities, indicating reduced liver damage, and mitigated oxidative stress by decreasing malondialdehyde levels while restoring superoxide dismutase activity. Western blot analysis revealed that PLE inhibited the phosphorylation of NF-κB pathway proteins (p65 and IκB) and suppressed TLR4 expression, highlighting its role in this inflammatory pathway. Additionally, PLE ameliorated D-GalN/LPS-induced gut microbiota dysbiosis, reducing Proteobacteria abundance and promoting beneficial genera such as Lactobacillus, Bifidobacterium, and Akkermansia. These findings suggest that PLE protects against liver inflammation and oxidative stress, while improving gut microbiota composition, offering potential therapeutic strategies for liver-related diseases.
Abstract
This study aimed to perform lactose hydrolysis using free and immobilised β-galactosidase in cow and sheep milk with different fat levels, followed by lactulose synthesis through the isomerisation of glucose to fructose using immobilised glucose isomerase. There was no significant difference (P > 0.05) in lactose hydrolysis (>95%) using the free enzyme in the different types of milk. In contrast, significant differences were observed for the hydrolysis of whole cow milk (WCM) and skimmed cow milk (SCM) with the immobilised enzyme (IE). WCM required a longer hydrolysis time (43 h). Thus, the fat content may have affected the lactose hydrolysis of bovine milk with immobilised β-galactosidase. No difference was observed for whole sheep milk and skimmed sheep milk hydrolysed by IE, possibly due to the presence of smaller fat globules, which did not affect the lactose hydrolysis. No significant differences were observed for lactulose synthesis in whole milk from the different species evaluated. The lactulose synthesis in cow and sheep milk by the enzymatic route may be a promising alternative, though further studies are required to optimise the production of this prebiotic in situ.
Abstract
The relevance of the subject under study is conditioned by numerous technological problems of providing livestock enterprises of the Republic of Kazakhstan with quality equipment for animal feeding and the associated need to develop and implement dosers for the preparation of compound feed mixture. This study introduces an innovative approach through the development and analysis of a novel dosing auger with an active return channel, which distinguishes itself from existing models by enhancing the precision and efficiency of feed preparation processes. The purpose of this study was to investigate the key parameters of the dosing auger with an active return channel for its further use in agricultural enterprises for preparation of compound feed. The findings of this study emphasise the significance of compliance of current trends in the improvement of prepared feed with the established zootechnical requirements from the standpoint of optimising the technological equipment used in this process. The key aspects of feed dosing sequence when using volumetric and mass dosing methods were considered. The main advantages of auger-in-auger dosers that distinguish them from all other types of dosers were described.
Abstract
Paste formulations of Volvariella volvacea (VV) were developed for its shelf-life enhancement. Egg stage fruiting bodies of mushrooms were used for preparation of salted, spiced, and their fermented pastes. Fermented paste formulations were developed using inoculation with Lactobacillus rhamnosus GG at 0.5% (w/w) and incubation for 2 days at 35 ± 2 °C. The unfermented VV paste was preserved with sodium benzoate of 0.065%, while fermented formulations were kept devoid of any chemical preservative. All formulations were packed in glass jars and retort bags, and thereafter treated in boiling water bath for one hour. The spiced, fermented VV paste scored highest in sensory evaluation and maximum crude protein and moisture contents, while lowest in contents of ash, fat, crude fibre, and carbohydrates. The fermented (salted and spiced) VV pastes showed variation in pH at 4 °C. Shelf life of 56 days was observed for fermented VV paste stored in glass jars under refrigerated conditions. The antioxidant analysis revealed that DPPH radical scavenging activity of fermented paste formulations increased significantly up to 21 days followed by gradual decrease until the 56th day, while total phenolic content of all paste formulations decreased with storage time. Hence, fermented spiced paste showed enhanced nutritive and antioxidative potential and shelf life up to 56 days.
Abstract
Gallnut honey, a typical Chinese medicinal commodity, is produced from the nectar of Rhus chinensis Mill. Gallnut honey is very popular with consumers because of its health effects and nutritional value, but its specific nutritional composition and medicinal components are not clear. Free amino acids are important nutritional components of honey, and can provide relevant information about the honey, such as geographical and botanical origin, as well as adulterations. The aim of this paper was to explore the free amino acid characteristics of gallnut honeys in different areas. 18 free amino acids from 17 places in China were determined by automatic amino acid analyser analysis, and the geographical characteristics were studied by principal component analysis and cluster analysis. Results showed that gallnut honey is rich in free amino acids. A total of 12 types of free amino acids were detected in all honey-producing areas, and the total amino acid content was 0.132–0.292 g/100 g. Due to its free amino acid content gallnut honey has high nutritional supplement potential, and exhibits good pharmacodynamic activity and special taste characteristics. Flavour-related free amino acids were primarily aromatic free amino acids and umami free amino acids. Pharmacodynamic free amino acids accounted for 61.22–83.85%. Results also showed that free amino acids contribute to the geographical tracing of honey, and samples of gallnut honey at different altitudes can be distinguished by cluster analysis.
Abstract
Penaeus vannamei is an important farm-raised shrimp species in China. However, the shrimp deteriorates quickly and has a short shelf life. The surface colour will change with the increase of spoilage, which can reflect its freshness. Therefore, a method for predicting Total Volatile Basic Nitrogen (TVB-N) content using a combination of a chromatic value and Near-Infrared (NIR) spectra is provided. This study explores which chromatic parameter is most efficient for freshness prediction from a data analysis perspective. The Levenberg–Marquardt Optimized Artificial Neural Network (LM-Optimised ANN), the Quadratic Support Vector Machine (Quadratic SVM), and Partial Least Squares Regression (PLSR) algorithms verified the idea. The combination of spectra and chromatic value b* can achieve a more accurate prediction of TVB-N content with RMSEP of 1.660 in Quadratic SVM, 1.337 in PLSR, and
Abstract
The study aimed to analyse the nutritional and antioxidant potential of corn silk based formulated tea. Four corn silk tea formulations were developed, one with corn silk only and three using different flavouring herbal/spicing agents, namely lemongrass, green cardamom, and cinnamon. Microwave dried corn silk powder and corn silk tea infusions were analysed for chemical composition. Organoleptic evaluation was also done for tea infusions. The results of chemical evaluation showed that corn silk powder is rich in protein (20.78%), dietary fibre (48.93%), calcium (139.5 mg/100 g), magnesium (163.9 mg/100 g), potassium (1,406 mg/100 g), total phenols (1,365 mg GAE/100 g), and flavonoids (370.8 mg QE/100 g). The antioxidant activity of corn silk powder was observed as 84.95% DPPH inhibition. Maysin content of corn silk powder was 3.65 mg/100 g. Chemical analysis of corn silk tea infusions showed that the addition of flavours significantly enhanced the minerals, total phenols, and flavonoids contents and the antioxidant activity as compared to the tea formulated with only corn silk powder. Results of organoleptic evaluation showed that all four corn silk tea formulations were highly acceptable. Overall improvement in nutritional and antioxidant contents was found in corn silk tea with added flavours.
Abstract
In the confectionery industry large quantities of palm fat in the fillings of chocolate products are used. Based on today's nutritional science results, it is desirable to replace palm oil with healthier fats. Oleogels can provide a kind of solution for this replacement. In our work the rheological, textural and thermal properties of oleogels containing high oleic sunflower oil, beeswax and monoglycerides were determined. In the samples we examined, the gelator concentrations were: 20% beeswax, 15% beeswax and 5% monoglyceride, 10% beeswax and 10% monoglyceride, 5% beeswax and 15% monoglyceride, and 20% monoglyceride. Based on our results, the oleogel containing 15% beeswax and 5% monoglyceride seems an eutectic crystal of beeswaxes and monoglyceride. It has relative high hardness, high storage modulus and high viscosity therefore it can replace the Chocofill filling fat, which contains mainly palm fat, used in large quantities in sweets.
Abstract
The spray is the primary method to apply pesticides to the crops. To provide enough coverage and deposition on target surfaces, the drop size produced during spray application must be precisely calibrated; nevertheless, it must not be too tiny to cause the undesirable phenomena known as “spray drift”. Spray drift is the movement of droplets in the atmosphere during or after spraying. The negative effect of spray drift can harm human health, livestock, and adjacent crops or can cause environmental pollution. To address this problem, a lot of work has been done. Previous studies on spray drift reduction approaches including factors promoting drift, drift measuring technologies, drift prediction models, and drift reduction technologies, were reviewed in this paper. Based on the literature review, future research and developments are projected. This review may provide guidance and reference to researchers for further development and improvement in drift reduction technologies.
Abstract
Introduction
Allergic contact dermatitis of the feet is a significant problem that affects the quality of life and requires attention from the medical community due to the number of studied and still unidentified allergens. The purpose of this review article is to summarize the available scientific data regarding the most common allergens that cause ACD of the feet.
Methods
Nickel sulphate, neomycin sulphate, thiuram mix and colophony occupy a significant place in the prevalence of allergies. The prevalence of sensitization to rubber and leather products can vary depending on the ethno-demographic characteristics of the country, as well as the specifics of a person's professional activity, such as the use of protective waterproof shoes, increased humidity of the microclimate, and atopy in anamnesis.
Results
Patch testing has been shown to be an important method for identifying allergens, however, not all footwear components are tested during patch testing with standard allergen series, requiring the use of patches made from patient shoe samples.
Conclusions
Expanding the scope of patch testing to include other possible allergens is important for the accurate diagnosis of ACD of the feet and a more detailed study of those allergens that were previously considered rare.
Abstract
The aims of this study were to prepare butter and honey flavoured tiger nut oil oleogels with beeswax oleogelator and to compare them with the unflavoured control sample. The oleogels prepared with 10 wt% beeswax and 0.5 wt% of each flavour addition were analysed for main physicochemical, thermal, and structural properties. In addition, a trained panel described the samples with 11 sensory descriptive (hardness, spreadability, roasted, liquefaction, rancid, waxy, nutty, butter, honey, cooling, and mouth coating) terms. It was found that the oleogels melt around 52 °C and included β′ type polymorphs. The sensory data proved that the samples were quite well spreadable and had enough hardness to stay as solid-fat samples at room temperature. Further, addition of butter and honey flavours masked the waxy attribute. In conclusion, flavoured tiger nut oil-beeswax oleogels were new and successful products, and future studies with food applications are suggested.
Abstract
In the presented research, oleosomes were obtained from unroasted hazelnuts in an aqueous environment with a pH of 9.5, adjusted using sodium bicarbonate. Model emulsions were then prepared to contain oleosomes in proportions of 15, 25, and 35%. Rheological and viscosity analyses were conducted on these model emulsions, focusing on elastic and viscous modulus values. The results of the analyses, which revealed that an increase in the oleosome content in the model emulsions correlated with a concurrent increase in emulsion viscosity as well as in the elastic (G′) and viscous (G″) moduli, demonstrate the significant impact of oleosome concentration on the rheological properties of the emulsions. All samples exhibited a G′ > G″ relationship, indicating their semi-solid nature. Moreover, an increase in the oleosome content was found to result in a higher consistency index for the product, while the flow index remained largely unchanged.