A New Leipothrix Species (Acari: Acariformes: Eriophyoidea) from Hungary on Zinnia elegans (Asteraceae)

A new vagrant species of phyllocoptine mites, Leipothrix nagyi n. sp. collected from Zinnia elegans (Asteraceae) is described and illustrated from Hungary. Further three eriophyoid species were recorded for the first time in Hungary, viz. Aceria hippophaena (Nalepa, 1898) found on Hippophaë rhamnoides , Epitrimerus cupressi (Keifer, 1939) collected from Cupressus sempervirens and Epitrimerus tanaceti Boczek et Davis, 1984 associated with Tanacetum vulgare . The female of E . tanaceti is re-described, while the male and nymph are described for the first time. Epicoxal the tip of Subcapitular (suboral) plate

The large family Asteraceae (Compositae) contains 1,911 plant genera with 32,913 accepted species names (The Plant List, 2013). Representatives of the family Asteraceae are a dominant feature of the Hungarian flora with 267 recognised species. According to Király (2009) it amounts to 9.8% of the current vascular plants of Hungary. An extraordinary range of eriophyoids occupy the plants of this family. In Hungary, 8% of 384 currently known eriophyoid species live on asteraceous hosts (Ripka, 2007). So far, no eriophyoid species has been recorded on the representatives of the genus Zinnia. Keifer (1966) erected the phyllocoptine genus Leipothrix (Acari: Acariformes: Eriophyidae) based on the following characters: basiventral femoral setae bv being absent on both leg pairs and having a central longitudinal ridge on the opisthosoma. Amrine et al. (2003) completed the characters with the presence of moderately long and bifurcate dorsal palp genual setae d. One Leipothrix species is known from Hungary: Leipothrix polygalae (Farkas, 1968) from Polygala amara subsp. brachyptera (Ripka, 2013). Recently several Epitrimerus species were reassigned to the genus Leipothrix based on branched pedipalp genual setae d and absence of femoral setae bv (Chetverikov, 2005;Petanović and Rector, *Corresponding author;e-mail: RipkaG@nebih.gov.hu The Author(s), 2020 Unauthenticated | Downloaded 04/10/21 05:56 PM UTC 2007). Leipothrix species (including the former Fletchmannia spp. which are synonyms according to Amrine et al., 2003) are known from the representatives of several plant families (e.g. Asteraceae, Chloranthaceae, Cyperaceae, Dipsacaceae, Pontederiaceae, Rubiaceae, Sinopteridaceae and Urticaceae) in North and South America, Asia, Africa and Europe (Davis et al., 1982;Amrine and Stasny, 1994;Amrine et al., 2003;Chetverikov, 2005;Petanović and Rector, 2007;Tan et al., 2016).

Materials and Methods
The eriophyoid mite fauna of different herbaceous and woody plant species was studied from plant samples collected in Budapest and different counties of Hungary, between 2013 and 2019. The plant material (including leaves, petioles, stems, buds, flowers and fruits) was collected and placed in plastic bags by E. Kiss, G. Ripka, J. Kontschán and Á. Szabó, then taken to the laboratory and examined under a stereo dissecting microscope (Zeiss Stemi 2000-C, Germany). Eriophyoid mites found were placed directly into 88% lactic acid with the aid of a bent insect pin. After clearing the specimens in lactic acid at room temperature, the mites were placed into Keifer's F-medium with sorbitol on microscope slides (Keifer, 1975). The slide preparations were dried and then sealed with commercial nail varnish (Upton, 1991). Specimens were examined with the aid of a research phase contrast compound microscope (Nikon Eclipse E600, Japan) equipped with a drawing tube (Nikon Y-IDT, Japan).
The generic classification follows Amrine et al. (2003), and comparisons were also made with new genera described since that publication. The terminology of external morphology and setal notation adopted for the morphological description follows mainly Lindquist (1996). The number of measured specimens (n) is given within parentheses in the description. All measurements of mites were made according to Amrine and Manson (1996) using an ocular micrometre eyepiece and are given in micrometres (μm). Measurements and means are rounded off to the nearest integer, when necessary. All measurements, unless specified otherwise, are lengths. In the description, each measurement of the holotype precedes the corresponding range for the paratypes. Range values are given in parentheses except in cases of constant value. For re-described females the mean and ranges, for males and immature stages, only the ranges are given.
The names of the plant taxa are used according to Király (2009  ridges ventrally bearing tiny round microtubercles; shield pattern consists of two faint, incomplete admedian lines beginning close to posterior margin of the shield, slightly diverging to rear margin, and a faint, short, incomplete submedian line along the tubercles of setae sc; median line absent. Prodorsal shield covered by dotting, punctate. Tubercles of setae sc 12 (12-14) ahead of rear shield margin, 15 (15-17) apart, setae sc 5 (4-5), directed up and projecting centrad. Epicoxal area with 5-6 thin ridges bearing tiny round microtubercles which reach the tip of frontal lobe. Subcapitular (suboral) plate cordate.
Host plant -Common zinnia, Zinnia elegans L. (Asteraceae); an annual ornamental plant from Mexico.
Relation to the host plant -The leaf vagrant mite was found on the upper side and underside of the leaves. No damage was caused.
Etymology -The species is named in honour of the late Dr. Barnabás Nagy (1921-2020), who was an eminent entomologist and ecologist. The gender is feminine.
Host plant -Tansy, Tanacetum vulgare L. (Asteraceae). Tansy is native to Hungary living in different plant associations, e.g. flood plain, wet fallow, and a segetal weed in field crops.
Relationship to the host -This mite caused no discernible symptoms on the host. The mite was found on the underside of the leaves. It is a new under surface leaf vagrant species for the fauna of Hungary.
Material examined -The re-described and illustrated female, the described male among 3 females (slide # 1490a), 29. ix. 2019, coll. Enikő Kiss. Other specimens were collected by E. Kiss in the same locality and time, one slide (# 1490b, with described male and nymph) was prepared from this material containing 1 male and 1 nymph from the leaves of the same plant. Slides are in the corresponding author's collection and deposited in the Directorate of Plant Protection, Soil Conservation and Agri-environment, National Food Chain Safety Office, Budapest, Hungary.

Remarks
There are some morphometric differences between the examined Hungarian Epitrimerus tanaceti specimens and the original description by Boczek and Davis (1984). We especially draw attention to the location of the microtubercles on the dorsal annuli: in the type specimens they are present on the first three dorsal annuli behind the prodorsal shield margin and in three bands on the middorsal and subdorsal ridges, whereas all dorsal annuli smooth in the specimens collected in Hungary. We also note that E. tanaceti has some design on the prodorsal shield, e.g. two incomplete admedian and two incomplete submedian lines (but they are indistinct on the majority of the examined specimens). The length of setae h2 (22 in holotype of E. tanaceti) significantly differs from that of the Hungarian specimens (70-75). Nevertheless the examined specimens belong to E. tanaceti because the vast majority of the morphometric data for female matches the original description by Boczek and Davis (1984).
The following two species are new for the fauna of Hungary as well: Eriophyes mali Nalepa, Malus domestica Borkh. (Rosaceae), Szár (Fejér county), coll. Á. Szabó, 23. vii. 2018. The mite was found under the epidermis on the lower side of the leaves, and caused brownish blisters and erineum on both sides of the leaves.