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Of the twenty table beet ( Beta vulgaris L. ssp. esculenta convar. crassa provar. conditiva ALEF.) cultivars investigated for several nutritionally important components (betaine, betanin, phenol, glucose, fructose, sucrose) formerly (Hájos et al., 2004), cultivar Ditroit having average quality parameters was chosen for in vitro animal experiments. The hyperlipidemic rat model is suitable to study the physiological effect of table beet on the metabolic alterations and the redox homeostasis in the liver. In fatty liver, as a consequence of hyperlipidemy, the redox homeostasis is strongly injured. General biologically active compounds of table beet have indirect lipid lowering effect and antioxidant properties. Therefore, the effect of lyophilised table beet powder was studied on altered lipid metabolism and redox parameters in hyperlipidemic rats. Hyperlipidemy was induced by a fat-rich diet, and both in the control group and in the hyperlipidemic group, animals were treated with the lyophilised table beet powder (2 g bwkg −1 ) added into rat chow for 10 days parallel with the feeding. Significant antioxidant activity of table beet was recorded in the hyperlipidemic liver. This phenomenon was expected because of the significant amount of betanin and the high total polyphenol content of the beet. Significant beneficial changes were also observed in the serum cholesterol level, alkaline phosphatase and alanine-aminotransferase activities, although a non-expected elevation was observed in the serum bilirubin level in hyperlipidemy. Non-specific H-donor activity was not changed, but protein related free SH-group concentration was decreased in the plasma. Serum triglyceride level was better after table beet treatment in normolipidemy, only. Change of redox-homeostasis was more favourable in the liver during the treatment. Diene conjugate content and the level of induced free radicals decreased during the table beet treatment in case of fatty liver. These changes were due to the bioactive components of the commercially available table beet. Consequently, table beet due to its specific qualities beneficially influences several metabolic pathways, therefore it can be considered as a functional food.
In our research we studied the occurrence of the main apple allergen coding gene-families (Mal d 1, Mal d 2, Mal d 3, Mal d 4) in 16 different and most preferably consumed apple varieties. After the DNA isolation by Wizard method the simple PCR reaction was used to examine the apple allergen-coding genes. To identify the presence of the four allergenic protein-coding genes two primer pairs were chosen. The presence of these allergens in most apple varieties could be confirmed. According to our results two varieties — Jonathan and Granny Smith — were found to contain the lowest amount of the coding genes of the allergenic apple proteins studied by us. Besides this, polymorph pattern was obtained by the use of Mal d 1 primer, which may lead to determine apple varieties with small amount of Mal d 1 allergens.The confirmation study of the presence of potential apple allergens by RNA and protein techniques is our plan in the near future.
The main objective of the DOSoReMI.hu (Digital, Optimized, Soil Related Maps and Information in Hungary) project is to significantly extend the potential, how demands on spatial soil related information could be satisfied in Hungary. Although a great amount of soil information is available due to former mappings and surveys, there are more and more frequently emerging discrepancies between the available and the expected data. The gaps are planned to be filled with optimized digital soil mapping (DSM) products heavily based on legacy soil data, which still represent a valuable treasure of soil information at the present time. The paper presents three approaches for the application of Hungarian legacy soil data in object oriented digital soil mapping.
The increasing consumer demand for less processed and more natural food products – while improving those products’ quality, safety, and shelf-life – has raised the necessity of chemical preservative replacement. Biopreservation refers to extended storage life and enhanced safety of foods using the natural microflora and (or) their antibacterial products. Chitinolytic enzymes are of biotechnological interest, since their substrate, chitin, is a major structural component of the cell wall of fungi, which are the main cause of the spoilage of food and raw plant material. Among the several organisms, many bacteria produce chitinolytic enzymes, however, this behaviour is not general. The chitinase activity of the lactic acid bacteria is scarcely known and studied.
The aim of the present study was to select Lactobacillus strains that have genes encoding chitinase, furthermore, to detect expressed enzymes and to characterise their chitinase activity. Taking into consideration the importance of chitin-bindig proteins (CBPs) in the chitinase activity, CBPs were also examined. Five Lactobacillus strains out of 43 strains from 12 different species were selected by their chitinase coding gene. The presence of the chitinase and chitin-biding protein production were confirmed, however, no chitinolytic activity has been identified.
A talajok tulajdonságainak javítása céljából végzett bioszénnel történő kezelések hatása a különböző fizikai, kémiai és biológiai tulajdonságú talajok esetében még nem teljesen ismert. Kísérleteinket homoktalajon végeztük az MTA ATK TAKI Őrbottyánban lévő kísérleti telepén, ahol kukoricát vetettek. Hét kezelést vizsgáltunk, négy ismétlésben. Három esetben a talaj különböző dózisban bioszenet és konstans dózisú műtrágyát tartalmazott (0,1 m/m%; 0,5 m/m%; 1 m/m%; jelölésük BC0,1M; BC0,5M; BC1,0M), három esetben pedig a fent említett bioszén dózisokat egységesen 10 t/ha komposzttal egészítettük ki (BC0,1K; BC0,5K; BC1,0K). Ezek mellett pedig kialakítottunk egy bioszén és komposzt mentes abszolút kontroll (K) kezelést is. Kutatásunk során talajszondákkal monitoroztuk a talajnedvességtartalmának alakulását, valamint statikus kamrás mintavételi eljárással a talajlégzést is mértük a kezelésekben.
A talajnedvesség éves átlagát nézve 1% bioszénnel és komposzttal kezelt parcella esetében a talaj nedvességtartalma nem szignifikáns mértékben növekedett a bioszén és komposzt mentes abszolút kontroll környezethez képest. Csapadékesemények alkalmával az 1% bioszenet és komposztot tartalmazó parcellában nőtt meg legjobban a talajnedvesség, illetve hasonlóan alakult a nedvességtartalom a 0,5% bioszénnel kezelt műtrágyás parcellában is. Csapadékesemények után az összes bioszenet és műtrágyát, illetve bioszenet és komposztot tartalmazó parcellában gyorsabban száradt ki a talaj a kontrollhoz képest. A csapadékban szegényebb, szárazabb időszak alkalmával egyedül az 1% bioszenet és komposztot tartalmazó kezelés talajnedvessége volt magasabb a kontrollhoz képest, a 0,5% bioszénnel és műtrágyával kezelt, komposzt mentes esetben a nedvesség hasonlóan alakult a kontrollhoz viszonyítva, az összes többi esetben jóval az alatt maradtak az értékek.
Összességében megállapítható, hogy a komposztot tartalmazó talajok érzékenyebben reagáltak a csapadékra, a legjobb vízgazdálkodást az 1% bioszén és komposzt kezelés esetében értük el. Önmagában a bioszén nagy mennyiségű (1,0 m/m%) adagolása nem volt egyértelműen talajnedvesség-növelő hatású.
A bioszén szén-dioxid forgalomra történő hatását a talajlégzés mérésével vizsgáltuk. A bioszénnel, valamint műtrágyával kezelt és a kontroll kezelések között csak néhány esetben volt különbség. A komposzttal kevert bioszén kezelések alkalmával hasonló eredményre jutottunk, mint a műtrágyával kevert bioszén esetében. Eredményeink alapján arra következtethetünk, hogy a talajlégzés nem függött a bioszén dózisától. A bioszén talajlégzésre gyakorolt hatása közvetett módon, a talajnedvesség befolyásolásán keresztül valósul meg, mivel bioszenet alkalmazva bizonyos esetekben a talajnedvesség emelkedett a kontrollhoz képest, ekkor a talajlégzés ugyancsak magasabb lett, amely jelenség a komposzttal kezelt esetekben jól megfigyelhető volt.
Depending on their origin, sedimentary phosphate rocks (PRs) may differ in their P solubility, and, as a consequence, in their agronomic effectiveness. The effect of six phosphate rocks (PR) - originating from Algeria (ALG), North Florida (FLO), North Carolina (NCA), Senegal (SEN) Morocco (MOR) and Hyperphosphate (HYP) with various P solubility (evaluated by 2% formic acid, 2% citric acid, and neutral ammonium citrate) - as well as single superphosphate (SSP) and superphosphate + lime (SSP + Ca) (each P source on 4 P levels, with doses of 0, 100, 400 and 1600 mg P 2 O 5 ·kg -1 soil) on the shoot yield of tillering stage spring barley, soil available P (i.e. H 2 O, Olsen, Bray1, Lakanen-Erviö (LE) and ammonium lactate (AL) extractable P contents) were studied in pot experiments set up with acidic sandy soil (Nyírlugos, Hungary) and acidic clay loam soil (Ragály, Hungary), both with low P supplies. The average spring barley shoot yield at the beginning of shooting was 95% higher on the colloid-rich acidic (pH KCl : 4.5) clay loam soil than on the colloid-poor acidic (pH KCl : 3.8) sandy soil. The differences in the solubility of phosphate rocks showed close correlation to the differences in P responses. On both soils, the correlation between total PR-P added and P responses in spring barley shoot yield was much weaker than that between neutral ammonium citrate soluble PR-P added and P responses in spring barley shoot yield. When phosphate rocks were applied as P sources, the comparison of soil test P methods showed a different picture on the two soils. In the case of the acidic sandy soil (Nyírlugos), the strongly acid LE-P (r² = 0.83) and AL-P (r² =0.74) tests gave the highest correlation coefficients with spring barley responses to P, while on the acidic clay loam soil (Ragály) these were achieved by the Olsen-P (r² = 0.88) and Bray1-P (r² =0.88) methods.