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To screen fecal samples for adenovirus antigens a genus-specific monoclonal antibody based enzyme immunoassay was developed. In a comparative analysis with commercial latex agglutination test, high sensitivity was demonstrated. The assay did not detect other viruses usually found in faeces suggesting its specificity. One hundred and eighty stool samples collected in Baranya County were tested and 13 (7.2%) of them showed reactivity. The application of our immunoassay combined with other, more sophisticated methods may help us to determine the serotype specificity of these adenovirus isolates and assess the importance of adenoviruses in viral gastroenteritis.

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Agrokémia és Talajtan
Authors:
Katalin Sárdi
,
P. Csathó
,
I. Sisák
,
E. Osztoics
,
P. Szűcs
, and
Á. Balázsy
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Summary  

Vanadium-48 was produced with an activity of 222 MBq (6 mCi) by the natTi(d,xn)48V nuclear reactions in the U-120 cyclotron. The energy of the irradiating beam was 13 MeV, its intensity 5 μA, and the metallic Ti target dimension 11’11 mm2 (0.1 mm thickness). For target cooling, circulated water in target backside was used. After 3 cooling days, only 48V and some 46Sc (T 1/2 = 83.8 d), produced by the side nuclear reaction 48Ti(d,α)46Sc were found in the target. For production of the radiotracer of 48V and for the preparation of source for standardization of 48V by 4πβ-γ coincidence, the Ti target was dissolved either in HF or in H2SO4. For both dissolving methods an ion-exchange separation procedure was developed.

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Abstract  

Understanding the characteristics of diffusion is essential in the assessment of radionuclide release through the backfill of waste repository. The diffusion behavior of 3H, 99Tc, 125I, 36Cl and 85Sr in granite, concrete and bentonite was studied in a specially designed diffusion cell. Diffusion coefficients (D, D a) and time-lag (t a) were measured, break-trough curves were plotted and experimental data were interpreted to predict diffusion rates. The results showed that tritium, iodine and chlorine could be considered as non-sorbing tracers, while technetium was weakly and strontium was strongly sorbed (no break-through was observed during the period of the experiments).

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Important differences have been reported in several countries about the level of concern in connection with food additives. In order to better understand the consumer preferences related to foodstuffs containing food additives, five hypotheses were tested with the help of a choice-based conjoint analysis. The study was performed in three European countries (Hungary, Romania, and Spain) having different risk perceptions concerning food additives. Two “model foodstuffs” were involved in the analysis: pre-packed sliced cheese (natural image) and chips (artificial image). For the creation of the conjoint cards, three attributes were selected: “preservatives” (natural/artificial), “packaging gases” (contains/does not contain) and “price” (average based on market data/+10%/+20%). Results were collected via Internet simultaneously and a total of 500 valid questionnaires were received in case of pre-packed cheese and 468 regarding chips. In the analysed countries “preservatives” had an outstanding importance — mainly in Hungary and Romania —, while “packaging gases” got lower importance during the shopping intention. “Natural preservatives” very positively contributed to the choosing of both pre-packed sliced cheese and chips. High level of willingness to pay was not observed, as well as the “price” was less important factor than the additives. Comparison of the data from three countries showed notable differences, which can determine the direction of the product planning and development.

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Hypertension is an increasing public health problem all over the world. Essential hypertension accounts for more than 90% of cases of hypertension. It is a complex genetic, environmental and demographic trait. New method in molecular biology has been proposed a number of candidate genes, but the linkage or association with hypertension has been problematic (lack of gene-gene and gene-environment interaction). It is well known that genetic influences are more important in younger hypertensives, because children are relatively free from the common environmental factors contributing to essential hypertension. The association studies compare genotype ferquencies of the candidate gene between patient groups and the controls, in pathways known to be involved in blood pressure regulation.This study examined three polymorphisms of these factors encoding genes (ET-1 G+5665T (Lys198Asn), endothelial nitric oxide synthase (eNOS) T-786C promoter polymorphism and 27-bp repeat polymorphism in intron 4) in adolescents with juvenile essential and obesity-associated hypertension.Significant differences were found in the G/T genotype of the ET-1 polymorphism in the hypertensive and obese+hypertensive patients (body mass index (BMI)>30). A strong association was detected between the BMI and the polymorphism of the ET-1 gene. It seems that ET-1 gene polymorphism plays a role in the development of juvenile hypertension associated with obesity. Although no significant differences were seen in the case of the eNOS promoter polymorphism and the eNOS 4th intron 27-bp repeat polymorphism. It seems that eNOS may play a role, but this is not the main factor in the control of blood pressure; it is rather a fine regulator in this process.This study with adolescents facilitates an understanding of the genetic factors promoting juvenile hypertension and obesity.

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Journal of Radioanalytical and Nuclear Chemistry
Authors:
P. Ormai
,
A. Fritz
,
J. Solymosi
,
I. Gresits
,
E. Hertelendi
,
Z. Szúcs
,
N. Vajda
,
Zs. Molnár
, and
P. Zagyvai

Abstract  

In the execution of disposal of low and intermediate level radioactive wastes, it is important to evaluate accurately the kind and quantity of each radionuclide in the wastes. For such an evaluation, correlation of non-gamma-emitting nuclides based on gamma-emitting nuclides is recommended and regarded as a practical method. This method necessitates a completion of a highly accurate and reliable nondestructive assay system of gamma-emitting nuclides for practical use. In 1992, in support of the new waste disposal program in Hungary, Paks NPP initiated a waste characterization program to determine the radiological properties of its radwastes. A segmented gamma scanning system has been set up to measure the gamma-emitting nuclides in 200 litre low level drums following in-drum compaction. In the framework of the program a radiochemical analysis sub-program was started to determine the long-lived non-gamma emitting radionuclides, mainly those listed in the US regulatory document (10CFR61). The radionuclides of interest have been3H,14C,90Sr,55Fe,59Ni,99Tc,129I and TRUs. Sample preparation techniques and measurement methods have been selected and used. Newly developed or adopted methods have been tested on real liquid radwaste streams such as concentrates, ion-exchange resin and sludge. The measurements taken so far have revealed brand new information and data on radiological composition of waste of WWER-type reactors. In the next stage of the characterisation program attempt will be made for providing correlation factors between the gamma and non-gamma-emitting radionuclides in different waste streams. Short description of the methods and results on waste inventory are given by highlighting the problem areas.

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Cereal Research Communications
Authors:
T. Tóth
,
T. Németh
,
A. Bidló
,
F. Dér
,
M. Fekete
,
T. Fábián
,
Z. Gaál
,
B. Heil
,
T. Hermann
,
E. Horváth
,
G. Kovács
,
A. Makó
,
F. Máté
,
K. Mészáros
,
Z. Patocskai
,
F. Speiser
,
I. Szűcs
,
G. Tóth
,
Gy. Várallyay
,
J. Vass
, and
Sz. Vinogradov
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