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Lycopene, found primarily in tomatoes, is a member of the carotenoid family and has potent antioxidant capability. The aims of the present study were: 1) to investigate the effect of grafting on lycopene content of tomatoes and 2) to evaluate the effects of different varieties on lycopene content (open-field with supporting-system, open-field with processing varieties) under the same ecological conditions. The effect of grafting on lycopene content was analysed in the case of two varieties, between the years 2001 and 2003. Lycopene content of tomato fruits decreased significantly by grafting. Ten commercial varieties of tomato produced in Hungary were examined for their lycopene content. Lycopene content of tomato turned out to be extremely diverse, and variable (63.0–155.0 mg/kg fresh weight). It was found that the variety of tomato is one of the most important determinants of lycopene content.

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Tomato is one of the most important sources of lycopene. The effect of water supply was investigated on lycopene content of Daniela F1

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A three years (2008, 2009, and 2011) open field experiment was conducted to study the effect of growing methods (organic and conventional) on the tomato yield components (Brix°, carbohydrate, organic acid, lycopene, and total polyphenols). Significantly higher Brix°, carbohydrate-, and lycopene content was found in conventional production. In spite of this, total phenolic content of tomato fruits was significantly higher in organic production. Our experiences show that it is probable, that the techniques used in the cultivation of organic crops — no artificial nutrients — could activate natural defence mechanisms in tomato plants, by increasing content of total polyphenol in the fruits.

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Pituitary adenylate cyclase activating polypeptide (PACAP) is involved in development and reproduction. We previously described elevated PACAP levels in the milk compared to the plasma, and the presence of its specific PAC1 receptor in the mammary gland. This study aimed to determine PACAP and vasoactive intestinal peptide (VIP) levels in female suckling lambs compared to ewe plasma and mammary gland, as well as their age-dependent alterations. mRNA expressions of PACAP, VIP, PAC1 receptor and brain-derived neurotrophic factor (BDNF) were quantified in the milk whey and mammary gland. PACAP38-like immunoreactivity (PACAP38-LI) was measured in plasma, milk whey and mammary gland by radioimmunoassay, VIP-LI by enzyme-linked immunoassay. PACAP38-LI was 5, 6 times higher in the milk compared to the plasma of lactating sheep. It significantly increased in the lamb plasma 1 h, but returned to basal level 2 h after suckling. However, VIP mRNA was not present in the mammary gland, we detected the VIP protein in the milk whey. BDNF mRNA significantly decreased with age to approximately 60% and 25% in the 3- and 10-year-old sheep respectively, compared to the 3-month-old lambs. No differences were found between mammary and jugular vein plasma PACAP and VIP concentrations, or during the daily cycle. We propose a rapid absorption of PACAP38 from the milk and/or its release in suckling lambs. PACAP accumulated in the milk might be synthesized in the mammary gland or secreted from the plasma of the mothers. PACAP is suggested to have differentiation/proliferation promoting and immunomodulatory effects in the newborns and/or a local function in the mammary gland.

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Acta Physiologica Hungarica
Authors: K. Csanaky, D. Reglődi, E. Bánki, I. Tarcai, L. Márk, Zs Helyes, T. Ertl, J. Gyarmati, K. Horváth, L. Sántik, and Andrea Tamás

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with special importance in reproductive and developmental processes. PACAP is found in two bioactive forms: PACAP27 and PACAP38. Recently, we have described that PACAP38 is present in high levels in the milk of human and ruminant animals. Breastfeeding is of utmost importance in proper nutrition of the newborn, but artificial nursing with infant formulas is necessary when breastfeeding is not available. Composition of the breast milk varies during the whole period of nursing and it shows differences at the beginning (foremilk) and the end of an actual suckling (hindmilk). The aim of this study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in different milk and infant formula samples by radioimmunoassay and to prove the presence of PACAP38 in the infant formula by mass spectrometry. We found similar PACAP38-LI in human mature foremilk and hindmilk samples, in the fresh and pasteurized cow milk and also in formulas. However, we found significantly higher PACAP38-LI in the hypoantigenic formula undergoing extensive hydrolysis compared to the non-hypoantigenic ones. Our results suggest that PACAP38 is relatively stable in the milk and it can withstand the manufacturing processes.

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