Authors:A. He, M. Ye, Z. Tang, S. Lu, Y. Gu, X. Fan, L. Zhao, and J. Gao
The recombination of hydrogen and oxygen in technical gaseous waste of nuclear power plants in enlarged scale experiment has
been studied on the basis of our previous work.1 The catalyst and its best operating conditions for recombination of hydrogen and oxygen determined in a small scale experiment
were demonstrated and tested. The results show that the data obtained in an enlarged scale experiment agreed well with that
of in a small scale test. The recombination rate of H2 and O2 was higher than 98.3% and 99.98% respectively. After recombination, the residual concentrations of H2 and O2 in waste gas were O2<3 ppm, H2<400 ppm. The Pd-Al2O3 catalyst and operating conditions determined for gaseous waste processing of nuclear power plants were satisfactory.
Authors:M. Lu, B. Wang, Zh. Li, Y. Fei, L. Wei, and Sh. Gao
Antifreeze protein (AFP) can lower the freezing point by inhibiting the growth of ice crystals. In this article, the thermal
hysteresis activity (THA) of a plant AFP was measured with differential scanning calorimetry (DSC). As is shown, when the
amount of ice in the sample was less than 5% THA of this AFP reached as high as 0.35°C. The secondary structure of this AFP
was studied with circular dichroism (CD). The CD spectrum from 195to 240 nm indicated a well-defined secondary structure consisting
11% α-helix, 34%antiparallel β-sheet and 55% random coil.
Authors:X.L. Qi, X.F. Li, F. He, L.Q. Hu, Y.G. Bao, J.R. Gao, and H.G. Wang
Thinopyrum intermedium, which has many useful traits, is valuable for wheat breeding. A new wheat-Thinopyrum addition line, SN100109, was developed from the progeny of common wheat cultivar Yannong 15 and Th. intermedium. It was resistant to most races of Blumeria graminis f. sp tritici (Bgt), which caused powdery mildew in wheat, and its reactions were different from the reactions of gene Pm40 and Pm43. Genomic in situ hybridization (GISH) and molecular marker analysis were used to identify the genomic composition of SN100109. GISH results showed that SN100109 was a wheat-Th. intermedium disomic addition line containing one pair of J chromosomes, and the resistance gene was located on the alien additional chromosomes of SN100109. And four molecular markers BE425942, BF482714, Xgdm93 and BV679214 which were assigned to homologous group 2, were specific molecular markers of the additional chromosomes. All the results indicated that SN100109 contained one pair of 2J chromosomes. SN100109 can be used as a novel germplasm source for introducing powdery mildew resistance genes to wheat in breeding programs.
Authors:S. Hu, H. Shu, J.L. Yuan, J.Y. Gao, P.Y. Mu, C.Z. Ren, W. Sang, L.C. Guo, and H.B. Chen
The objective of this study was to evaluate the eﬀect of wheat–oat ﬂour ratio on the physical properties and β-glucan characteristics of extrudates. Results showed that increasing the wheat–oat ﬂour ratio resulted in a decrease in the water solubility index (r2=0.8567) and hardness (r2=0.9316), whereas the expansion ratio (r2=0.9307) and water absorption index (r2=0.9061) increased. Wheat ﬂour generally caused an increase in L values from 57.81 to 62.94 providing bright samples. Few cells were observed at high wheat–oat ﬂour ratios under a scanning electron microscope, and a smooth surface was noted. Meanwhile, the total (r2=0.9867) and soluble (r2=0.9848) β-glucan contents were inversely proportional to the wheat–oat ﬂour ratio. Extrudates with added wheat ﬂour had a high molecular weight, but wheat ﬂour had no signiﬁcant (P<0.05) eﬀect on the viscosity of β-glucan extracts. Conclusively, incorporation of wheat ﬂour at a wheat–oat ﬂour ratio of 2.33 provides ready-to-eat food based on whole oat ﬂour, on account of improving the texture and providing suﬃcient β-glucan contents (0.806 g/100 g) without signiﬁcantly aﬀecting β-glucan viscosity.
Authors:A. He, M. Ye, Z. Tang, S. Lu, H. Cao, Y. Gu, X. Fan, L. Zhao, and J. Gao
The recombination of hydrogen and oxygen in technical gaseous waste of nuclear power plants has been studied. A highly efficient catalyst for reacting H2 with O2 to form water was prepared. Various operating conditions and factors affecting the recombination of H2 and O2 were tested and the best conditions were determined. Results show that the Pd–Al2O3 catalyst prepared had very good characteristics. The recombination rate of H2 and O2 was higher than 98.3% and 99.9%, respectively. After recombination, residual concentrations of H2 and O2 in waste gas were O2<3 ppm, H2<400 ppm. The Pd–Al2O3 catalyst and operating conditions determined for gaseous waste processing of nuclear power plants were satisfactory.
Authors:Z. Fang, D. Sun, J. Gao, M. Guo, L. Sun, Y. Wang, Y. Lıu, R. Wang, Q. Deng, D. Xu, and R. Gooneratne
Shewanella putrefaciens supernatant was found to increase the virulence factors of Vibrio parahaemolyticus by eﬃciently degrading its acylhomoserine lactone (AHL). To further reveal the regulation mechanism and its key degrading enzyme, a potential AHL-degrading enzyme acylase (Aac) from S. putrefaciens was cloned, and the inﬂuences of temperature, pH, protein modiﬁers, and metals on Aac were tested. Aac was signiﬁcantly inﬂuenced by temperature and pH, and exhibited the highest AHL-degrading activity at temperatures of 37 °C and pH of 8. Mg2+ and Fe2+ can further increase the AHL-degrading activity. 10 mM EDTA inhibited its activity possibly by chelating the co-factors (metals) required for Aac activity. Tryptophan and arginine were identiﬁed as key components for Aac activity that are critical to its AHL-degrading activity. This study provides useful information on Aac and for V. parahaemolyticus control.
Authors:X.R. Yu, L. Zhou, J. Zhang, H. Yu, D.R. Gao, B.Q. Zhang, F. Xiong, Y.J. Gu, and Z. Wang
This study was conducted to compare structural development and biochemical accumulation of waxy and non-waxy wheat (NW) caryopses. The caryopses’ microstructure of the waxy wheat (WW) and NW cultivars at different developmental stages were observed under light, fluorescence, and scanning electron microscope. The results were as follows: Compared with NW,WWhad a shorter maturation duration, which was reflected in several following characteristics. Programmed cell death of the pericarp began earlier, and the chlorophyll-containing layer in the pericarp was smaller. Vacuoles in chalazal cells accumulated more tannins at different developmental stages. Starch granules and protein bodies in the endosperm showed a higher accumulation level in developing caryopses, and aleurone cells were larger in size with larger numbers of aleurone grains. An analysis of the element content indicated that the mineral elements Mg, P, K, and Ca exhibited a higher content, while the heavy elements Cr, Cd, and Pb exhibited a lower content in the aleurone layer.