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The mediodorsal prefrontal cortex (mdPFC) is a key structure of the central glucose-monitoring (GM) neural network. Previous studies indicate that intracerebral streptozotocin (STZ) microinjection-induced destruction of local chemosensory neurons results in feeding and metabolic alterations. The present experiments aimed to examine whether STZ microinjection into the mdPFC causes metabolic deficits. To do so, glucose tolerance test (GTT) and measurements of plasma metabolites were performed in STZ-treated or control rats. Intraperitoneal D-glucose load was delivered 20 min or 4 weeks following the intracerebral microinjection of STZ or saline (acute or subacute GTT, respectively). The STZ-treated rats displayed acute glucose intolerance: at the 120th min of the test, blood glucose level of these rats was significantly higher than that of the ones in the control group. When determining the plasma level of various metabolites, 30 min following the intracerebral STZ or saline microinjection, the triglyceride concentration of the STZ-treated rats was found to be reduced compared with that of the control rats. The GM neurons of the mdPFC are suggested to be involved in the organization of complex metabolic processes by which these chemosensory cells contribute to adaptive control mechanisms of the maintenance of homeostasis.

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Acta Alimentaria
Authors:
E. Horvath-Szanics
,
J. Perjéssy
,
A. Klupács
,
K. Takács
,
A. Nagy
,
E. Koppány-Szabó
,
F. Hegyi
,
E. Németh-Szerdahelyi
,
M.Y. Du
,
Z.R. Wang
,
J.Q. Kan
, and
Zs. Zalán

The increasing consumer demand for less processed and more natural food products – while improving those products’ quality, safety, and shelf-life – has raised the necessity of chemical preservative replacement. Biopreservation refers to extended storage life and enhanced safety of foods using the natural microflora and (or) their antibacterial products. Chitinolytic enzymes are of biotechnological interest, since their substrate, chitin, is a major structural component of the cell wall of fungi, which are the main cause of the spoilage of food and raw plant material. Among the several organisms, many bacteria produce chitinolytic enzymes, however, this behaviour is not general. The chitinase activity of the lactic acid bacteria is scarcely known and studied.

The aim of the present study was to select Lactobacillus strains that have genes encoding chitinase, furthermore, to detect expressed enzymes and to characterise their chitinase activity. Taking into consideration the importance of chitin-bindig proteins (CBPs) in the chitinase activity, CBPs were also examined. Five Lactobacillus strains out of 43 strains from 12 different species were selected by their chitinase coding gene. The presence of the chitinase and chitin-biding protein production were confirmed, however, no chitinolytic activity has been identified.

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Interventional Medicine and Applied Science
Authors:
Szaniszló Z. Jávor
,
K. Shanava
,
E. Hocsák
,
M. Kürthy
,
J. Lantos
,
B. Borsiczky
,
I. Takács
,
Sz. Horváth
,
B. Balatonyi
,
S. Ferencz
,
A. Ferencz
,
E. Rőth
, and
Gy. Wéber

Abstract

Increased intra-abdominal pressure during laparoscopy leads to hypoxia due to reduced blood flow. Aim of our study was to investigate whether preconditioning can reduce this negative effect of the pneumoperitoneum. Fifty female Wistar rats were used, divided into 5 groups. I: Sham operation (Sham), II: conventional pneumoperitoneum (PP), III: transvaginal pneumoperitoneum (TV), IV: preconditioning for 2.5 minutes in two cycles (Pre 2.5), V: preconditioning for 5 minutes (Pre 5). Malondialdehyde (MDA), reduced glutathione (GSH), sulfhydrylgroup (SH-) concentrations, superoxide-dismutase (SOD) and mieloperoxidase (MPO) activity, and anti-apoptotic pathway marker p-AKT level and inflammatory cytokine TNF-α were measured. SOD activity and GSH concentration were decreased in PP and TV groups comparing to Sham and preconditioning groups. MPO activity was decreased also in PP and TV groups comparing to the Sham group but in the preconditioning groups it has remained high. MDA concentration in plasma was increased in PP and TV groups comparing to Sham and preconditioning groups. There was no difference in the case of blood MDA and SH-concentrations between groups. Anti-apoptotic pathway marker p-AKT level was decreased in the TV group comparing to the sham and preconditioning groups. TNF-α level was increased in TV and preconditioning groups compared to the sham group. According to the results preconditioning can reduce negative effects of pneumoperitoneum.

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A talajok tulajdonságainak javítása céljából végzett bioszénnel történő kezelések hatása a különböző fizikai, kémiai és biológiai tulajdonságú talajok esetében még nem teljesen ismert. Kísérleteinket homoktalajon végeztük az MTA ATK TAKI Őrbottyánban lévő kísérleti telepén, ahol kukoricát vetettek. Hét kezelést vizsgáltunk, négy ismétlésben. Három esetben a talaj különböző dózisban bioszenet és konstans dózisú műtrágyát tartalmazott (0,1 m/m%; 0,5 m/m%; 1 m/m%; jelölésük BC0,1M; BC0,5M; BC1,0M), három esetben pedig a fent említett bioszén dózisokat egységesen 10 t/ha komposzttal egészítettük ki (BC0,1K; BC0,5K; BC1,0K). Ezek mellett pedig kialakítottunk egy bioszén és komposzt mentes abszolút kontroll (K) kezelést is. Kutatásunk során talajszondákkal monitoroztuk a talajnedvességtartalmának alakulását, valamint statikus kamrás mintavételi eljárással a talajlégzést is mértük a kezelésekben.

A talajnedvesség éves átlagát nézve 1% bioszénnel és komposzttal kezelt parcella esetében a talaj nedvességtartalma nem szignifikáns mértékben növekedett a bioszén és komposzt mentes abszolút kontroll környezethez képest. Csapadékesemények alkalmával az 1% bioszenet és komposztot tartalmazó parcellában nőtt meg legjobban a talajnedvesség, illetve hasonlóan alakult a nedvességtartalom a 0,5% bioszénnel kezelt műtrágyás parcellában is. Csapadékesemények után az összes bioszenet és műtrágyát, illetve bioszenet és komposztot tartalmazó parcellában gyorsabban száradt ki a talaj a kontrollhoz képest. A csapadékban szegényebb, szárazabb időszak alkalmával egyedül az 1% bioszenet és komposztot tartalmazó kezelés talajnedvessége volt magasabb a kontrollhoz képest, a 0,5% bioszénnel és műtrágyával kezelt, komposzt mentes esetben a nedvesség hasonlóan alakult a kontrollhoz viszonyítva, az összes többi esetben jóval az alatt maradtak az értékek.

Összességében megállapítható, hogy a komposztot tartalmazó talajok érzékenyebben reagáltak a csapadékra, a legjobb vízgazdálkodást az 1% bioszén és komposzt kezelés esetében értük el. Önmagában a bioszén nagy mennyiségű (1,0 m/m%) adagolása nem volt egyértelműen talajnedvesség-növelő hatású.

A bioszén szén-dioxid forgalomra történő hatását a talajlégzés mérésével vizsgáltuk. A bioszénnel, valamint műtrágyával kezelt és a kontroll kezelések között csak néhány esetben volt különbség. A komposzttal kevert bioszén kezelések alkalmával hasonló eredményre jutottunk, mint a műtrágyával kevert bioszén esetében. Eredményeink alapján arra következtethetünk, hogy a talajlégzés nem függött a bioszén dózisától. A bioszén talajlégzésre gyakorolt hatása közvetett módon, a talajnedvesség befolyásolásán keresztül valósul meg, mivel bioszenet alkalmazva bizonyos esetekben a talajnedvesség emelkedett a kontrollhoz képest, ekkor a talajlégzés ugyancsak magasabb lett, amely jelenség a komposzttal kezelt esetekben jól megfigyelhető volt.

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Cereal Research Communications
Authors:
K. Végh
,
E. Osztoics
,
P. Csathó
,
J. Csillag
,
L. Radimszky
,
G. Baczó
,
M. Magyar
,
T. Takács
,
A. Lukács
, and
M. Karátsonyi
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Interventional Medicine and Applied Science
Authors:
K. Shanava
,
Sz. Horváth
,
F. Karl-Hermann
,
Sz. Jávor
,
I. Takács
,
B. Balatonyi
,
S. Ferencz
,
A. Ferencz
,
E. Rőth
, and
György Wéber

Abstract

Introduction:The Natural Orifice Transluminal Endoscopic Surgery (NOTES) is an abdominal operation carried out with flexible endoscopic instruments and their advanced versions via natural orifices. The NOTES causes theoretically less pain, operative stress, shorter hospitalization, quicker recovery and it is scarless. The aim of this investigation was to evaluate the feasibility and safety of transgastric small bowel resection. Materials and methods: Seven domestic pigs were investigated and entered the study; body weight 25 kg in average. General anesthesia was performed. One trocar was used for laparoscopic observations during gastrotomy and transgastric penetration with the gastroscope, later for assistance with a grasper for manipulation of the bowel loops and for the application of the linear stapling device. Then the needle knife was used to complete a 1.5 cm long incision in the gastric wall. The gastroscope was advanced into the peritoneal cavity and a small bowel loop was identified and elevated with a flexible grasper. Through the second channel of the scope a coag grasper was used to dissect the mesentery. Then a linear stapler was inserted through the assisting trocar and the resection was performed. Afterwards the bowel ends were opened with the needle knife and the stapler was reinserted for a side-to-side anastomosis. The specimen was removed via the stomach. Gastric closure was completed by laparoscopic mini-instruments through the stapler-port. All special events and all problems were prospectively documented. Follow-up was performed over 2 weeks and body weight was recorded. Then a laparoscopy was performed to document adhesions or abscess formation. Finally the animals were sacrificed to evaluate further evidence of infection or adhesions. Results: The operations were carried without complications, there was no case of letal outcome. On the 12th day the abdominal laparoscopic revision was carried out, after the revision in one case adhesion could be detected, no other kinds of complications were noticed. Conclusion: According to our results, the transgastric small bowel resection is a safe procedure, but further special instruments are needed. These experimental procedures should be evaluated carefully and critically in clinical practice.

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Agrokémia és Talajtan
Authors:
Péter Csathó
,
E. Osztoics
,
J. Csillag
,
T. Lengyel
,
L. Gonda
,
L. Radimszky
,
G. Baczó
,
M. Magyar
,
K. R. Végh
,
M. Karátsonyi
,
T. Takács
,
A. Lukács
, and
T. Németh

Depending on their origin, sedimentary phosphate rocks (PRs) may differ in their P solubility, and, as a consequence, in their agronomic effectiveness. The effect of six phosphate rocks (PR) - originating from Algeria (ALG), North Florida (FLO), North Carolina (NCA), Senegal (SEN) Morocco (MOR) and Hyperphosphate (HYP) with various P solubility (evaluated by 2% formic acid, 2% citric acid, and neutral ammonium citrate) - as well as single superphosphate (SSP) and superphosphate + lime (SSP + Ca) (each P source on 4 P levels, with doses of 0, 100, 400 and 1600 mg P 2 O 5 ·kg -1 soil) on the shoot yield of tillering stage spring barley, soil available P (i.e. H 2 O, Olsen, Bray1, Lakanen-Erviö (LE) and ammonium lactate (AL) extractable P contents) were studied in pot experiments set up with acidic sandy soil (Nyírlugos, Hungary) and acidic clay loam soil (Ragály, Hungary), both with low P supplies.  The average spring barley shoot yield at the beginning of shooting was 95% higher on the colloid-rich acidic (pH KCl : 4.5) clay loam soil than on the colloid-poor acidic (pH KCl : 3.8) sandy soil. The differences in the solubility of phosphate rocks showed close correlation to the differences in P responses. On both soils, the correlation between total PR-P added and P responses in spring barley shoot yield was much weaker than that between neutral ammonium citrate soluble PR-P added and P responses in spring barley shoot yield. When phosphate rocks were applied as P sources, the comparison of soil test P methods showed a different picture on the two soils. In the case of the acidic sandy soil (Nyírlugos), the strongly acid LE-P (r² = 0.83) and AL-P (r² =0.74) tests gave the highest correlation coefficients with spring barley responses to P, while on the acidic clay loam soil (Ragály) these were achieved by the Olsen-P (r² = 0.88) and Bray1-P (r² =0.88) methods. 

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Acta Botanica Hungarica
Authors:
P. Török
,
T. Miglécz
,
O. Valkó
,
K. Tóth
,
A. Kelemen
,
Á.-J. Albert
,
G. Matus
,
A. Molnár V
,
E. Ruprecht
,
L. Papp
,
B. Deák
,
O. Horváth
,
A. Takács
,
B. Hüse
, and
B. Tóthmérész

In the present paper we report original thousand-seed weight data for the flora of the Pannonian Basin. Our goal was to demonstrate the usefulness of seed weight databases by analysing seed weight data in relation to social behaviour types and life forms. We specifically asked the following questions: (i) how the seed weights are related to social behaviour type categories; (ii) how the life form of the species influences seed weight differences between respective social behaviour types? Own weight measurements are provided for 1,405 taxa; and for 187 taxa we published seed weight data for the first time: these were mostly endemics, orchids and/or species with Pontic, Caspian or continental distribution. Several taxonomic or functional groups are underrepresented in our database, like aquatic plants, rare arable weeds and sub-Mediterranean species. Problematic taxa, some difficult-to-harvest species or species with low seed production and cultivated adventives are also underrepresented. We found that the plant strategies expressed by social behaviour types were significantly different in terms of seed weights. The lowest seed weight scores were found for natural pioneers, whereas the highest ones were found for adventives and introduced cultivated plants. Short-lived herbaceous species had significantly higher seed weight scores than herbaceous perennials. No significant differences were found between specialists and generalists within the stress tolerant group. We found that short-lived graminoids possess heavier seeds than perennial graminoids, perennial and annual forbs. Naturalness scores were negatively correlated with seed weights. Our findings showed that seed collections and databases are not only for storing plant material and seed weight data, but can be effectively used for understanding ecological trends and testing plant trait-based hypotheses. Even the identified gaps underline the necessity of further seed collection and measurements.

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Acta Botanica Hungarica
Authors:
P. Török
,
E. Tóth
,
K. Tóth
,
O. Valkó
,
B. Deák
,
B. Kelbert
,
P. Bálint
,
Sz. Radócz
,
A. Kelemen
,
J. Sonkoly
,
T. Miglécz
,
G. Matus
,
A. Takács
,
V. A. Molnár
,
K. Süveges
,
L. Papp
,
L. Papp Jr.
,
Z. Tóth
,
B. Baktay
,
G. Málnási Csizmadia
,
I. Oláh
,
E. Peti
,
J. Schellenberger
,
O. Szalkovszki
,
R. Kiss
, and
B. TÓthmérész

For understanding local and regional seed dispersal and plant establishment processes and for considering the ecotypes and other forms of specific variability, hard data of locally or regionally measured traits are necessary. We provided newly measured seed weight data of 193 taxa, out of which 24 taxa had not been represented in the SID, LEDA or BiolFlor databases. Our new measurements and formerly published data of locally collected seed weight records together covers over 70% of the Pannonian flora. However, there is still a considerable lack in seed weight data of taxonomically problematic genera, even though they are represented in the Pannonian flora with a relatively high number of species and/or subspecies (e.g. Sorbus, Rosa, Rubus, Crataegus and Hieracium). Our regional database contains very sporadic data on aquatic plants (including also numerous invasive species reported from Hungary and neighbouring countries) and some rare weeds distributed in the southwestern part of the country. These facts indicate the necessity of further seed collection and measurements.

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