Authors:H. Enginar, P. Unak, F. Biber Müftüler, F. Lambrecht, E. Medine, S. Yolcular, A. Yurt Kilcar, B. Seyitoğlu, and I. Bulduk
The aim of this study was to synthesize a glucuronide conjugated morphine derivative which could be labeled with 131I, as a radiopharmaceutical, and to investigate its radiopharmaceutical potential using biodistribution studies in male Albino
Wistar rats. Morphine was extracted from dry capsules of the opium poppy (Papaver somniferum L.). It was conjugated with UDP-glucuronic acid by using UDP-glucuronyl transferase (UDPGT) enzyme rich microsomes, purified
by high performance liquid chromatography (HPLC) and characterized by nuclear magnetic resonance (NMR), infrared (IR) spectroscopy
and liquid chromatography mass spectroscopy (LC-MS/MS). Normal and receptor blockage biodistribution studies were performed
in male Albino Wistar rats. The results of the tissue distribution studies showed that 131I labeled morphine glucuronide (131I-mor-glu) uptake in the small intestine, large intestine and urinary bladder was higher than in the other tissues of the
rats in the blocked receptor and unblocked receptor. A greater uptake of the radio labeled substance was observed in the hypothalamus
and mid brain than in the other branches of the rats’ brains.
Authors:H. Enginar, P. Ünak, F. Lambrecht, F. Biber Müftüler, E. Medine, S. Yolcular, A. Yurt, B. Seyitoğlu, and I. Bulduk
Codeine which was extracted from dry capsules of the opium poppy (Papaver somniferum) was purified by HPLC (High Performance Liquid Chromatography) and characterized by NMR (Nuclear Magnetic Resonance) and
IR (Infrared) spectroscopy techniques. The purified compound was labeled with 131I and biodistribution studies were performed in rats. Radioiodinated codeine distributed uniformly in the cerebellum, m.pons,
striatum and hypothalamus while the other branch of brain and Stomach, urinary bladder, and small intestine uptakes were significantly
higher than other tissues.