Search Results

You are looking at 21 - 30 of 40 items for

  • Author or Editor: Lin Zhang x
  • Refine by Access: All Content x
Clear All Modify Search

Abstract

Li-ion batteries with LiFePO4/C composites are difficult to be charged at low temperatures. In order to improve the low temperature performance of LiFePO4/C power batteries, the charge–discharge characteristics were studied at different temperatures, and a new charging mode under low temperature was proposed. In the new charging mode, the batteries were excited by current pulses with the charge rates between 0.75 C and 2 C, while the discharge rates between 3 and 4 C before the conventional charging (CC–CV). Results showed that the surface temperature of Li-ion battery ascended to 3 °C at the end of pulse cycling when the environment temperature was −10 °C. Comparing with the conventional charging, the whole charge time was cut by 36 min (23.4%) and the capacity was 7.1% more at the same discharge rate, respectively.

Restricted access

Abstract  

The biological behavior of Technetium-99m-L,L-Ethylenedicysteine (Tc-99m-EC)was compared with that of Technetium-99m-Mercaftoacetyltriglycine (Tc-99m-MAG3) and Iodine-131-hippuran (1131-OIH) in mice and rabbits. Tc-99m-EC showed a more rapid urinary excretion and less retention in kidney, liver, intestines and blood in comparison to Tc-99m-MAG3 and I-131-OIH. Urinary excretion decreased after probenecid treatment, indicating active tubular transport. In human bodies, the renal scintigraphy with Tc-99m-EC, Tc-99m-MAG3 and Tc-99m-DTPA were comparable. The promising results of the animal and human studies suggest that Tc-99m-EC may be a useful alternative to Tc-99m-MAG3 and I-131-OIH for renal functional studies.

Restricted access

Abstract

Lactate, a metabolite of exercise, plays a crucial role in the body. In these studies, we aimed to investigate the role of G protein-coupled receptor 81 (GPR81), a specific receptor for lactate, in regulating lipid storage in the gastrocnemius muscle of rats. To achieve this, we measured the impact of sodium 3-hydroxybutyrate (3-OBA) concentration and time on the cAMP-PKA signaling pathway in the gastrocnemius muscles of rats. Our investigation involved determining the effects of administering 3-OBA at a concentration of 3 mmol L−1 just 15 min before exercise. As expected, exercise led to a notable increase in intramuscular lactate concentration in rats. However, injecting 3-OBA prior to exercise yielded intriguing results. It not only further augmented the cAMP-PKA signaling pathway but also boosted the expression of lipolysis-related proteins such as hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL). Simultaneously, it decreased the expression of fat-synthesizing proteins, including acetyl CoA carboxylase (ACC) and fatty acid synthase (FAS), while increasing the protein expression of cytochrome c oxidase subunit Ⅳ(COX Ⅳ) and the activity of citrate synthetase (CS). Unfortunately, there was no significant change observed in intramuscular triglyceride (IMTG) content. In summary, our findings shed light on the role of lactate in partially regulating intramuscular triglycerides during exercise.

Restricted access

Abstract  

In the present study, two novel 99mTc nitrido dithiocarbamate complexes containing heterocyclic linkage, the bis(N-2-(1-piperidyl)ethyl dithiocarbamato) nitrido technetium-99m complex 99mTcN(PPEDTC)2 and bis(N-2-(1-pyrrolidino)ethyl dithiocarbamato) nitrido technetium-99m complex 99mTcN(PREDTC)2 have been synthesized by the reduction of 99mTcO4 into [99mTcN]2+ with stannous chloride in the presence of succinic dihydrazide and propylenediamine tetraacetic acid, followed by the addition of the corresponding dithiocarbamate ligands. The radiochemical purity of the complexes were over 90% as measured by thin layer chromatography (TLC). In vitro studies showed that the complexes possessed good stability. Their partition coefficients indicated that they were lipophilic complexes. Biodistribution in mice showed that the 99mTcN(PPEDTC)2 complex accumulated in brain with high uptake and good retention. As compared with the 99mTcN(PREDTC)2 complex, the former showed much higher brain uptake than the latter.

Restricted access

The common wheat line, YW243, developed in our research group, was tested for the resistances of barley yellow dwarf virus (BYDV), powdery mildew (Pm) and stripe rust in field, and was analyzed by molecular markers for convenient trace of the resistant genes in breeding. Genomic in situ hybridization (GISH) analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay further demonstrated that YW243 was a homozygous multiple translocation line of Triticum aestivum, Thinopyrum intermedium and Secale cereale (T7DS·7DL-7XL & 1BL·1RS). The disease resistance test and marker analysis showed that YW243 carried seven resistance genes to the three diseases, including Bdv2 to BYDV on 7DL-7XL, Pm4 to powdery mildew on 2AL, Yr2, Yr9, Sr 31 and Lr26 and a new Yr to stripe rust on 7B, 1BL, 1RS and 2BL. Restriction fragment length polymorphism (RFLP) markers Xpsr687 and Xwg380 , sequence tagged site (STS) marker STS 1700 , simple sequence repeat (SSR) markers Xgwmc364 and Xgwm582 , SSR markers Xgwm388 and Xgwm501 can be used as diagnostic tools to track Bdv2, Pm4, Yr2, Yr9 and Yr in YW243 , respectively; and two amplified fragment length polymorphism (AFLP) markers M54E63 - 700 and M54E64 - 699 can also be used to select Yr in YW243 .

Restricted access
Acta Chromatographica
Authors:
Lianguo Chen
,
Qingwei Zhang
,
Yijing Lin
,
Xiaojie Lu
,
Zuoquan Zhong
,
Jianshe Ma
,
Congcong Wen
, and
Cheng Ding

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine the hapepunine in mouse blood, and the pharmacokinetics of hapepunine after intravenous (1.0 mg/kg) and intragastric (2.5, 5, and 10 mg/kg) administrations was studied. Delavinone was used as an internal standard. The UPLC ethylene bridged hybrid (BEH) C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 0.1% formic acid with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring (MRM) mode was used for quantitative analysis of hapepunine in electrospray ionization (ESI) positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. The verification method was established in accordance with the US Food and Drug Administration (FDA) bioanalytical method validation guidelines. In the concentration range of 1–1000 ng/mL, the hapepunine in the mouse blood was linear (r 2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 12%, the inter-day precision CV was less than 14%. The accuracy ranged from 91.7% to 109.3%. The average recovery was higher than 76.7%, and the matrix effect was between 86.0% and 106.4%. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of hapepunine in mice. The absolute bioavailability of hapepunine was 22.0%.

Open access
Acta Chromatographica
Authors:
Shuanghu Wang
,
Zixia Lin
,
Ke Su
,
Jing Zhang
,
Lijing Zhang
,
Zhimou Gao
,
Zhiyi Wang
,
Jianshe Ma
, and
Xianqin Wang

The rats were randomly divided into paraquat group, curcumin treatment group, and pirfenidone treatment group. The concentration of paraquat in rat plasma was determined by an ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method over the range of 10–2000 ng mL−1. Chromatographic separation was achieved on a BEH HILIC (2.1 mm × 100 mm, 1.7 μm) column. The mobile phase was consisted of acetonitrile and 10 mm ammonium formate buffer (containing 0.1% formic acid) with gradient elution pumped at a flow rate of 0.4 mL min−1. Protein precipitation with acetonitrile was used as sample preparation. Compared with the paraquat group, there is statistical toxicokinetic difference for curcumin treatment group and pirfenidone treatment group, AUC(0 − t) decreased (P < 0.05), clearance (CL) increased (P < 0.05) for curcumin or pirfenidone treatment group, and C max decreased (P < 0.05) for curcumin treatment group. The results showed that treatment by curcumin and pirfenidone could relieve acute paraquat poisoning in rats.

Open access
Cereal Research Communications
Authors:
Zhengqiang Ma
,
Shulin Xue
,
Feng Lin
,
Shaohua Yang
,
Guoqiang Li
,
Mingzhi Tang
,
Zhongxin Kong
,
Yong Cao
,
Dongmei Zhao
,
Haiyan Jia
,
Zhengzhi Zhang
, and
Lixia Zhang

Wangshuibai is an indigenous scab resistance germplasm originated from Jiangsu, China. To characterize the genetic basis of scab resistance in this germplasm, QTLs for type I and type II resistances were detected using a recombinant inbred line (RIL) population created by single seed descent from Nanda2419 × Wangshuibai and a molecular marker map of more than 4000 cM constructed using RAPD, SSR and STS markers. The major QTLs for type I resistance in Wangshuibai were mapped to chromosomes 4BL and 5AS, and those for type II resistance were mapped to chromosomes 3BS. In addition, a QTL on chromosome 2B showed association with both types of resistance. These QTLs were verified with QTL nearisogenic lines. We found, by mapping QTLs for agronomical traits in the same population, that on chromosomes 4BL and 5AS the scab resistance QTLs co-located with QTLs for plant height, thousand grain weight or flag leaf width. However, these associations could be break down by recombinant selection. We concluded that Wangshuibai is a valuable scab resistance gene resources and marker assisted selection would be of great help for its better utilization.

Restricted access

A reliable isotope dilution method for the determination of chloramphenicol (CAP) in drinking water was developed by using an evaporation preparative step. Each sample was monitored by ultrahigh-pressure liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS) using an electrospray ionization interface (ESI) in negative ion modes. Recoveries of spiked samples were in the range from 93.2% to 95.7% with intra-day relative standard deviation lower than 6.7% and inter-day relative standard deviation lower than 8.2%. Limit of quantification (LOD) was 0.002 ng/mL. The developed method was successfully applied to the analysis of CAP in drinking water of Shannan region of Tibet.

Open access