Authors:Yang Yan-Zhao, Sun Si-Xiu, Yang Yong-Hui, Zhang Lei, Yu Shao-Ning, and Bao Bo-Rong
The synergic extraction of uranium(VI) from nitric acid solution with petroleum sulfoxides (PSO) and tri-n-butyl phosphate (TBP) mixture has been studied. It has been found that maximum synergic extraction effect occurs if the molar
ratio of PSO to TBP is two to three. The composition of the complex of synergic extraction is UO2(NO3)2·TBP·PSO. The formation constant of the complex isKPT=8.19. The effect of extractant concentration, nitric acid concentration, salting-out agent concentration and temperature
on the extraction equilibrium of uranium(VI) was also studied.
Authors:Guo Jin-xin, Sun Xuan, Yin Zhi-lei, Li Xue-mei, Yu Hai-yun, Yang Yan-zhao, and Sun Si-xiu
The mechanism of solvent extraction of gold(III) in hydrochloric acid media with quaternary ammonium salt octadecyl dimethyl benzyl ammonium chloride dissolved in chloroform was studied. The influences of temperature, NaCl and HCl concentraction on the extraction equilibrium were also investigated. The extraction reaction is exothermic (
H° = -28.53±0.15 kJ/mol) and log Kex = 5.47±0.04.
Authors:Q. Chen, M. Cao, W. Xiang, Q. Sun, J. Zhang, R. Hou, Z. Yan, Z. Yang, J. Liu, and J. Zhao
The forward and reverse cDNA subtractive libraries before and after the toxic effect of α-amanitin were constructed by suppression subtractive hybridization and randomly selected clones from each subtractive library were screened by PCR and dot blot hybridization. A total of 85 genes with altered expression were finally identified, with 41 genes from the forward library and 44 from the reverse library. Subsequently, the antagonistic effects of candidate traditional Chinese medicines were evaluated based on the genetic transcription levels of the genes with significant altered expression, including Catnβ, Flt3-L, IL-7r and Rpo2-4. The results indicated that
(L.) Gaert and
had significant down-regulated effects on the transcription level of Catnβ that was up-regulated by α-amanitin, and the two herbs also up-regulated the transcription levels of Flt3-L and Rpo2-4.
(L.) had significant up-regulated effects on the IL-7r that was down-regulated by α-amanitin. These preliminary studies suggested that
were effective antagonists against the toxicity of α-amanitin.
Authors:Chang-Liang Yan, Qi-En Yang, Guang-Bin Zhou, Yun-Peng Hou, Xue-Ming Zhao, Zhi-Qiang Fan, Man-Qing Liu, Lin Liu, and Shi-En Zhu
The present study was designed to investigate the cryotolerance of
fertilised (IVF) mouse embryos at various preimplantation developmental stages. IVF mouse embryos were vitrified by the open-pulled straw (OPS) method. After warming, embryos were morphologically evaluated and assessed by their development to blastocysts, hatched blastocysts or term. The results showed that a high proportion (93.3–100.0%) of vitrified embryos at all developmental stages were morphologically normal after recovery. The developmental rate of vitrified 1-cell embryos to blastocyst (40.0%) or hatched blastocyst (32.7%) or term (9.3%) was significantly lower than that from other stages (P < 0.05). Vitrified embryos from 2-cell to early blastocyst stage showed similar blastocyst (71.8–89.5%) and hatched blastocyst rates (61.1–69.6%) and could develop to term without a significant loss of survival compared with those of fresh embryos (P > 0.05). Vitrified 2-cell embryos showed the highest survival rate
(50.6%, 88/174), compared with that from other stages (9.3–30.5%, P < 0.05). The data demonstrate that the OPS method is suitable for the cryopreservation of IVF mouse embryos from 2-cell stage to early blastocyst stage without a significant loss of survival. Embryos at the 2-cell stage had the best tolerance for cryopreservation in the present study.