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Acta Alimentaria
Authors:
G. Gulyás
,
L. Czeglédi
,
B. Béri
,
S. Harangi
,
E. Csősz
,
Z. Szabó
,
T. Janáky
, and
A. Jávor

The aim of this study was to investigate the differences in the proteome patterns of musculus longissimus dorsi between Charolais bulls slaughtered at 500 kg and 700 kg live weight using two-dimensional difference in gel electrophoresis (2D-DIGE) and liquid chromatography-mass spectrometry (LC-MS). Three hundred fifty protein spots were visualised on gels of which 10 showed different expression levels (P<0.05) between groups. After mass spectrometric analysis of spots, beta-enolase (ENO3) in five different spots, alpha-enolase (ENO1), triosephosphate isomerase (TPI1) in two different spots, alpha-actin (ACTA1), and heat shock protein beta-1 (HSPB1) were identified. ENO3, ENO1, TPI1, and ACTA1 had higher expression levels in bulls of 700 kg live weight group. ENO3, ENO1, and TPI1 are involved in energy metabolism, while ACTA1 is a structure protein in skeletal muscle. Up-regulation of heat shock protein beta-1 (HSPB1), which protein is reported to have correlation with tenderness, was observed in 500 kg weight group. Our result demonstrates that proteomic tools are useful in identifying markers associated with muscle development.

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Abstract

In view of the poor aqueous solubility of nifluminic acid (NIF), the aim of this article was to improve its solubility and dissolution rate through the preparation of formulations based on hydroxypropyl β-cyclodextrin (HPβCD) and polyvinylpyrrolidone K25 (PVP K25), a combination of carriers which has been advantageously used for a similar purpose with various hydrophobic drugs. Ternary systems of NIF, HPβCD, and PVP K25 were prepared in different drug to CD to PVP ratios by physical mixing, kneading, microwave irradiation, and co-evaporation. Differential scanning calorimetry, thermogravimetric analysis, hot stage microscopy, Fourier transform infrared spectroscopy, and X-ray powder diffractometry were used to investigate the resulting solid-state interactions. The results showed that the solid state of the drug in the amorphous or crystalline ternary combinations influenced both the solubility and the dissolution rate of NIF.

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Abstract  

The regional distribution of copper and other trace elements was determined in the brain of a patient deceased in Wilson's disease against a control brain. The heterogeneous distribution of copper was checked by scanning electron microscope equipped with an X-ray microanalyzer.

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Abstract  

Recommended k0-factors and related nuclear data for use in (n, ) activation analysis are given for 72 isotopes. In addition the basic nuclear constants and experimental parameters needed in the k0 standardization method are reviewed. For convenient data reduction, computer programs were developed.

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Acta Alimentaria
Authors:
P. Penksza
,
R. sárosi
,
R. Juhász
,
K. Manninger-kóczán
,
B. Szabó-Nótin
,
L. Szakács
, and
J. Barta

The aim of the present study was to investigate whether the Jerusalem artichoke powder produced by a simple drying method is suitable for replacing inulin in dairy products and in fat replacer mixtures. Rheological properties of milk drinks were tested by rotational method measuring the flow curve and fitting the Herschel-Bulkley model. The Jerusalem artichoke powder showed similar rheological behaviour as the commercially available inulin and proved to be a more effective thickener in milk drinks as indicated by the higher consistency values at the same concentration. Panelists found milk drinks prepared with Jerusalem artichoke powder to have similar sensorial quality as prepared with inulin. Fat replacer mixtures were tested by oscillatory tests using amplitude sweep method. The samples containing Jerusalem artichoke powder had lower complex viscosity and initial G’ and G” values indicating weaker gel forming properties compared to inulin. However, lower slope of G’ and G” indicated their better spreadability. The organoleptic texture properties of fat replacer prepared with Jerusalem artichoke powder proved to be slightly better than that of the inulin containing mixture. Based on our results, the Jerusalem artichoke powder seems to be suitable to replace inulin as a natural additive in certain food products.

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Acta Alimentaria
Authors:
R. Sárosi
,
K. Manninger-Kóczán
,
P. Penksza
,
R. Juhász
,
B. Szabó-Nótin
,
L. Szakács
, and
J. Barta

The aim of the present study was to investigate whether Jerusalem artichoke powder produced by a simple drying method is suitable for replacing inulin as a dietary fibre in bread. Baking probe was performed using 0, 5, and 10% Jerusalem artichoke (JA) powder and 2.5 and 5% inulin (IN) on wheat flour weight basis. Functional properties of bread loafs were tested by Stable Micro System TA TX2i Texture Analyser (SMS) after 0, 24, 48, and 72 h storage. It was concluded that using inulin as a dietary fibre did not deteriorate the baking quality of bread, however, shelflife slightly decreased. Jerusalem artichoke powder compared to inulin had similar effect on the baking properties of bread. Based on our results, JA powder proved to be a promising alternative for fibre enhancement in bread.

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Acta Alimentaria
Authors:
A. Kisbenedek
,
Sz. Szabo
,
E. Polyak
,
Z. Breitenbach
,
A. Bona
,
L. Mark
, and
M. Figler

Oilseeds are very popular edibles that are often used to enhance the fibre content of baked goods, and specific types are used for preserving and seasoning. Polyphenol-related researches have been receiving growing attention in the last 20 years, especially the ones concentrating on stilbenoids. In previous studies, resveratrol concentrations have been determined from oilseeds such as peanut.The aim of our research was to define the composition of oilseeds with a focus on the bioactive compounds, more specifically the resveratrol.Research took place in 2010–2011 at the University of Pécs, Medical School, using non-random, convenience sampling. Oilseeds studied in the research were: sunflower seed, roasted peanut, un-roasted peanut, sesame seed, pumpkin seed, almond, linseed, bio white mustard seed, bio black mustard seed, mustard seed of foreign provenance, and wild black mustard seed. All of these oilseeds can be purchased from trade. Samples used in the research were obtained from the producers and collectors. High Performance Liquid Chromatography (HPLC) was used for the measurements.Summarising our results, it can be stated that each type of oilseed analysed in our research can be regarded as good sources of resveratrol. The highest level of resveratrol was detected in the sunflower seeds (0.00398±0.0001 mg g−1), almonds (0.00176±0.00021 mg g−1), roasted peanut (0.00206±0.00013 mg g−1), and wild black mustard seeds (0.0023±0.0007 mg g−1).

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Abstract  

A non-destructive activation analytical method developed for the determination of the axial concentration distributions of Al, Si and K residual additives in sintered tungsten rods is briefly described. A 14 MeV neutron generator is used to produce the reactions27Al(n, p)27Mg,28Si(n, p)28Al and39K(n, 2n)38K and the activities are compared with standards. A weighted least-squares fitting program is used for peak area determination of the scintillation spectra. A scheme of the irradiating and measuring arrangement with a pneumatic sample-transfer system is presented. The system is applicable to large-scale routine analysis and lends itself well to automation. The results of preliminary measurements with this arrangement are given.

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The sugar composition of floral nectar was studied in 17 sour cherry cultivars in Újfehértó in 1997-2000. All samples contained the three most frequent sugar components: glucose, fructose and sucrose, similarly to our previous data. The ranking of the floral nectar in sour cherry cultivars based on sucrose content was the following: 'Újfehértói fürtös' and 'Pándy 48' reached the threshold value of bee visitation in at least three seasons, and a significant amount of sucrose was detected in the flowers of 'Érdi jubileum AB' and 'Érdi bőtermő' as well. The above cultivars proved to be the most valuable in Újfehértó from the viewpoint of apiculture. According to the ranking based on fructose content, which considers human sensation of taste, the most favourable cultivars were 'Újfehértói fürtös', 'Meteor USA' and 'Korai pipacs'. Based on total sugar content the secretory products preferred by bees were those of 'Újfehértói fürtös' (in three seasons); 'Korai pipacs', 'Érdi nagygyümölcsű', 'Sárándi S/Gy', 'Debreceni bőtermő', 'Kántorjánosi 3', 'Montmorency' and 'Meteor USA' (in two seasons). The ratio of nectar sugars, based on the Baker-quotient, S/(G+F), was sucrose-dominant at least in one season in 'Érdi jubileum AB', 'Érdi nagygyümölcsű' and 'Pándy 48'; hexose-rich in 'Korai pipacs', 'Kántorjánosi 3' and 'Montmorency'; all other cultivars had a sucrose-rich nectar. The nectar of all studied sour cherry cultivars possessed a composition preferred by bees. The basis of bees' nectar preference is the ratio, quantity and concentration of nectar sugar components, which were influenced by the effects of season to a high degree, differing from data in literature. From the viewpoint of nectar composition and concentration the most favourable temperature was around 20 °C.

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There are extensive data on effects of antifungal agents on the plant pathogens, especially on Fusariums spp. species. However, investigations on the interaction of chemicals and the treated cultivars are rare. The aim of the study was to test two types of fungicide mixtures, azoxystrobin-propiconazole, and prothioconazole-tebuconazole, which are applied in wheat cultivars intensively, on six fodder maize hybrids that were infected with Fusarium proliferatum in the R1 growth stage in a field trial. The effect of the fungicide treatment was tested on the starch content and antifungal, antioxidant polyphenols of the kernels in the R3–R4 and R6 stage of the cultivars. The level of the fungal presence and the fumonisin concentration of the kernels were increased significantly under the artificial infection. The fumonisin concentration was variable at the R6 stage of the hybrid maize kernels. The treatment with prothioconazole and tebuconazole was found to be suitable when it was done before flowering, while the azoxystrobin-propiconazole treatments were equally successful before and after maize flowering considering the decreasing fumonisin concentration of the kernels. Both fungicide mixtures, when they were applied after maize flowering, affected the starch biosynthesis to the R3–R4 stage significantly. Meanwhile, azoxystrobin-propiconazole also significantly affected the antioxidant flavone/flavanol contents from the R3–R4 stage to the R6 stage.

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