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  • Author or Editor: Enikő Tóth x
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Most of the beneficial biological effects (e.g. neurosedative, antioxidant, and antimicrobial) of black horehound ( Ballota nigra L.) are because of its phenylpropanoid glycoside content. A rapid TLC-densitometric method with acceptable accuracy has been established for quantitative analysis of the most characteristic components of black horehound. Experiments were performed to establish the optimum conditions for densitometric measurement and for storage of extracts. Practical application of the elaborated method is reported for five plant extracts.

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Different parts of three Ballota species ( B. nigra, B. hirsuta , and B. rupestris ) have been analyzed for phenylpropanoid content by means of a simple extraction technique and a newly established TLC-densitometric method. The compounds studied were verbascoside (VE), forsythoside B (FB), and caffeoyl-malic acid (CM). The largest amounts were detected in B. rupestris - CM (0.30%) and VE (4.58%) in the leaves and FB (3.0%) in the roots, followed by B. nigra ; the smallest amounts were found in B. hirsuta - 0.08% CM, 1.64% VE, and 0.91% FB in the leaves. The absence of caffeoylmalic acid was characteristic of the roots only. B. nigra , the common representative of the genus in Hungary, was chosen for thorough study of variation of phenylpropanoids during the vegetative period. Amounts of the phenylpropanoids increased during the main and secondary flowering periods in June (FB, 6.00%; VE, 8.50%; CM, 0.45% in the leaves) and September (FB, 3.80%; VE, 7.00%;, CM, 0.35% in the leaves).

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Stachys palustris L. (Lamiaceae) contains the iridoids harpagide and acetylharpagide. The presence of other components, for example aucubin, can be revealed by TLC and RP HPLC. A readily applicable simple TLC-densitometric method for analysis of aqueous extracts of the plants has been established for comparative studies of aucubin-like iridoids in Stachys palustris .

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