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- Author or Editor: F.S. Mohammed x
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Abstract
The nutritional and therapeutic benefits of plants are outstanding. Wild mustard (Sinapis arvensis L.) collected from different regions was analysed for its phenolic, flavonoid, protein, and biological activity levels. In this case, the plant ethanol extract was obtained using a soxhlet apparatus. Rel assay kits and the DPPH test were used to assess the plant's antioxidant activity. The agar dilution test was used to determine antimicrobial efficacy. A549 lung cancer cells were used in an MTT assay to measure antiproliferative activity. The Folin−Ciocalteu reagent was used to quantify the total phenolic content of the sample. The amount of flavonoids was determined using an aluminium chloride test. The amount of protein was calculated using the AOAC's standard technique. Based on the research conducted, it was shown that the maximum total antioxidant status (TAS) value for the ethanol extract of wild mustard from various places was 5.232 ± 0.047 mmol L−1. At a concentration of 2 mg mL−1, DPPH activity was measured to be 82.06 ± 1.01. The maximum levels of total phenolic, flavonoid, and protein were 80.57 ± 2.19 mg g−1, 154.07 ± 2.79 mg g−1, and 7.75 ± 0.24%, respectively. Doses of 25–100 μg mL−1 of plant extracts were effective against fungal strains, whereas doses of 50–200 μg mL−1 were beneficial against bacterial strains. The plant extracts were shown to have potent antiproliferative properties. It was found that wild mustard's phenolic, flavonoid, protein, and biological activity levels varied according to the location from which it was gathered. It was also concluded that wild mustard had significant biological activity.
Abstract
In this study, antioxidant, oxidant, antimicrobial, and antiproliferative activities of Asparagus acutifolius L. and Asparagus officinalis L., known for their nutritional properties, were determined. In this context, methanol (MeOH) and dichloromethane (DCM) extracts of plants were obtained. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined using Rel Assay kits. Antimicrobial activities of plant extracts were determined against the test microorganisms using the agar dilution method. Antiproliferative activity was tested on the lung cancer cell line A549. As a result of the studies, it has been determined that the plant species have high antioxidant potential. In addition, it was observed that the antifungal potentials of plant extracts are high. Antiproliferative activity was determined to be at high level in both plant species. As a result, it has been determined that A. acutifolius and A. officinalis have medical potential and can be used as natural agents in pharmacological designs.