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Resection and subsequent end-to-end anastomosis of the windpipe is a tried-and-tested acceptable method for the surgical treatment of segmental defects. There are a variety of different techniques for tracheal end-to-end anastomosis, but controversial reports highlight the fact that the suturing technique of the anastomosis is still subject of debate. We aimed to show the postoperative effects of the continuous and simple interrupted suturing technique respectively on the tracheal cartilage using differential scanning calorimetry. Transsection and subsequent reanastomosis of the cervical trachea was performed in 14 adult beagle dogs. The trachea was anastomized with continuous or simple interrupted sutures respectively depict no change in microcirculation after the resection of the trachea, but significant decrease following the completion the anastomosis with continuous sutures. Conventional histological analysis did not show any marked postoperative change in the tracheal cartilage but our DSC scans clearly demonstrated the differences between the intact cartilages and the ones involved in the anastomosis.

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Journal of Thermal Analysis and Calorimetry
Authors: L. Benkő, J. Danis, M. Czompo, R. Hubmann, A. Ferencz, G. Jancsó, Z. Szántó, A. Zólyomi, F. Könczöl, Á. Bellyei, E. Rőth, and D. Lőrinczy


Massive bleeding from oesophagus varices presents a life threatening complication of liver cirrhosis. No effective method of treatment is available until now, that would guarantee high grade of patient wellness during the conditioning and investigation phase until the definitive treatment could be introduced. The fact that we have not found any report in the literature about self-expandable metal stents (SEMS) application in acute variceal bleeding had encouraged us to use stents usually used for oesophageal malignancy and furthermore develop a special stent for this individual indication. The aim of this study was to evaluate the tissue response to oesophagus stent designed for stop acute variceal bleeding in animal experiment in compare with another stent used for iatrogenic treatment of different strictures of the oesophagus. Tissue oxygen saturation (StO2) measurement was performed before and after the implantation of the stents. Macroscopic and histological investigations of the stented oesophagus segments were observed after 10 days. Differential scanning calorimeter (DSC) is a well-established method for the demonstration of thermal consequences of local and global conformational changes in biological systems, but it has never been used for the investigation of the oesophagus. According to our results the thermal denaturation of intact oesophagus, its mucosa and muscle fragments revealed significant differences compared to healthy sample in favour of the new stent.

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