This work deals with the leaf blade anatomical structures of the broad-leaved fescue taxa, natural populations of temperate forage grasses, collected from various habitats in Central Europe. The following anatomical features were examined and quantitatively characterised: the ribs of leaves, type of the mesophyll, number of the sclerenchyma ribs, quantity of sclerenchyma tissue, presence of the colourless cells, number of bulliform cells, stomatal complexes and presence of papilla number. It was concluded that the anatomical structures and the main leaf blade characteristics of broad-leaved fescues show several differences between the taxa of Schenodorus and Drymanthele subgenus. The populations of the Schenodorus subgenus present heterogeneous mesophyll, with radial parenchyma, and colourless cells. Strong correlations exist between the bulliform cells and the quantity of sclerenchyma and papillae. The populations of Drymanthele subgenus also present heterogeneous mesophyll, but this is not evident for every taxa and populations. The radial parenchyma and the colourless cells are not specific. There is a positive correlation between the bulliform cells and the quantity of sclerenchyma. The comparative leaf anatomical analysis, could contribute to the better understanding of taxonomic and anatomical diversity in this group.