Authors:L. Benkő, J. Danis, R. Hubmann, G. Kasza, Éva Gömöri, Erzsébet Rőth, and D. Lőrinczy
Massive bleeding from esophagus varices presents a life threatening complication of portal hypertension. No effective method
of treatment is available until now, that would guarantee high grade of patient wellness during the conditioning and investigation
phase until the definitive treatment could be introduced. The aim of this study was to evaluate the tissue response to esophagus
stents - designed for manage acute variceal bleeding - in animal experiment.
Self-expandable nitinol stents were introduced into the esophagus of six porcines. Another twelve porcines were undergone
the same procedure, using the new biodegradable stents made of PDO (polydioxanon). Histological investigations of the stented
esophagus segments were observed after 2 and 4 weeks at the first 12 animals. To monitor the time of stent degradation, histology
were performed 7 and 12 weeks after the implantation in the biodegradable group (3–3 animals). Differential scanning calorimetric
examination was performed in all esophagus samples.
Focal erosion of the esophagus segments was more explicit in the nitinol group at the histology. On the 7th week all of the biodegradable stent were in the stomach and on the 12th week these were completely solved. DSC examination showed significant alterations in the structure of the esophagus in both
stented group compared to the healthy control.
This experiment showed that the new self-expandable stents are safety and suitable procedure without deterioration of the
esophageal wall. According to our DSC results the thermal denaturation of intact esophagus, its mucosa and muscle fragments
revealed significant differences compared to healthy sample in favour the new biodegradable stent. Safety and efficiency in
the experimental model had encouraged us to apply this method successfully patients with bleeding esophagus varices. The long
term goal is to show that stent placement could be an effective way of decreasing or stabilising the acute bleeding from ruptured
esophagus varices in cirrhotic patients.
Authors:L. Benkő, J. Danis, M. Czompo, R. Hubmann, A. Ferencz, G. Jancsó, Z. Szántó, A. Zólyomi, F. Könczöl, Á. Bellyei, E. Rőth, and D. Lőrinczy
Massive bleeding from oesophagus varices presents
a life threatening complication of liver cirrhosis. No effective method of
treatment is available until now, that would guarantee high grade of patient
wellness during the conditioning and investigation phase until the definitive
treatment could be introduced.
The fact that we have not found any report in the
literature about self-expandable metal stents (SEMS) application in acute
variceal bleeding had encouraged us to use stents usually used for oesophageal
malignancy and furthermore develop a special stent for this individual indication.
The aim of this study
was to evaluate the tissue response to oesophagus stent designed for stop
acute variceal bleeding in animal experiment in compare with another stent
used for iatrogenic treatment of different strictures of the oesophagus. Tissue
oxygen saturation (StO2) measurement was performed
before and after the implantation of the stents. Macroscopic and histological
investigations of the stented oesophagus segments were observed after 10 days.
calorimeter (DSC) is a well-established method for the demonstration of thermal
consequences of local and global conformational changes in biological systems,
but it has never been used for the investigation of the oesophagus. According
to our results the thermal denaturation of intact oesophagus, its mucosa and
muscle fragments revealed significant differences compared to healthy sample
in favour of the new stent.