Search Results

You are looking at 1 - 4 of 4 items for

  • Author or Editor: Judit Szepesi x
  • Refine by Access: All Content x
Clear All Modify Search

The hypersensitive response (HR), a type of programmed cell death (PCD) during biotic stress is mediated by salicylic acid (SA). The aim of this work was to reveal the role of proteolysis and cysteine proteases in the execution of PCD in response of SA. Tomato plants were treated with sublethal (0.1 mM) and lethal (1 mM) SA concentrations through the root system. Treatment with 1 mM SA increased the electrolyte leakage and proteolytic activity and reduced the total protein content of roots after 6 h, while the proteolytic activity did not change in the leaves and in plants exposed to 0.1 mM SA. The expression of the papain-type cysteine protease SlCYP1, the vacuolar processing enzyme SlVPE1 and the tomato metacaspase SlMCA1 was induced within the first three hours in the leaves and after 0.5 h in the roots in the presence of 1 mM SA but the transcript levels did not increase significantly at sublethal SA. The Bax inhibitor-1 (SlBI-1), an antiapoptotic gene was over-expressed in the roots after SA treatments and it proved to be transient in the presence of sublethal SA. Protease inhibitors, SlPI2 and SlLTC were upregulated in the roots by sublethal SA but their expression remained low at 1 mM SA concentration. It is concluded that in contrast to leaves the SA-induced PCD is associated with increased proteolytic activity in the root tissues resulting from a fast up-regulation of specific cysteine proteases and down-regulation of protease inhibitors.

Restricted access

In this study PTEs, [potentially toxic elements (Cr, Cu, Mn, Ni, Pb, and Zn)] were investigated in the upper layer of floodplain soils that occurred as a result of accident in the area of two mine tailings in Northwestern Romania. A large amount of sediment was deposited on the soil of floodplains along the Hungarian section of River Tisza, which could represent a threat to the environment. Floodplain soil samples were collected from four locations in Hungary from an area of the river stretching to about 250 km. BCR (Bureau Communautaire de Référence) sequential extraction method was used to analyze both post-flood and present samples. Most of the analyzed elements (Cd, Cr, Cu, Ni, Pb, Zn) were found in the residual fraction, but there is a notable soluble amount in hydroxylammonium chloride extractable fraction. The results allow a comparison of the changes that have taken place over time, in addition to serving as a basis for further studies.

Open access

The plant hormone ethylene or the gaseous signalling molecule nitric oxide (NO) may enhance salt stress tolerance by maintaining ion homeostasis, first of all K+/Na+ ratio of tissues. Ethylene and NO accumulation increased in the root apices and suspension culture cells of tomato at sublethal salt stress caused by 100 mM NaCl, however, the induction phase of programmed cell death (PCD) was different at lethal salt concentration. The production of ethylene by root apices and the accumulation of NO in the cells of suspension culture did not increase during the initiation of PCD after 250 mM NaCl treatment. Moreover, cells in suspension culture accumulated higher amount of reactive oxygen species which, along with NO deficiency contributed to cell death induction. The absence of ethylene in the apical root segments and the absence of NO accumulation in the cell suspension resulted in similar ion disequilibrium, namely K+/Na+ ratio of 1.41 ± 0.1 and 1.68 ± 0.3 in intact plant tissues and suspension culture cells, respectively that was not tolerated by tomato.

Restricted access

Myelodysplasiás syndroma ritka társulása chronicus myeloid leukaemiával és transzformációja acut myeloid leukaemiába

Rare association of myelodisplastic syndrome and chronic myeloid leukemia and transformation into acute myeloid leukemia

Hematológia–Transzfuziológia
Authors:
J. Péter Dombi
,
Judit Csomor
,
Zorka Barborják
,
Botond Tímár
,
Péter Szakál
,
Ágota Szepesi
, and
Zsófia Simon

Bevezetés

Bár a chronicus myeloid leukaemia ritkán társulhat más daganatos betegségekhez, myelodysplasiás syndroma utáni kialakulásáról irodalmi közlést nem találtunk. 71 éves férfi anamnézisében ischaemiás szívbetegség, ulcus ventriculi szerepelt. 2019-ben pancytopenia miatt indult kivizsgálása 5q delécióval járó myelodysplasiás syndromát igazolt, mely miatt lenalidomid kezelést indítottunk, ennek hatására vérképe rendeződött. Nyolc kezelési ciklus után jelentkező leukocytosis felvetette acut myeloid leukaemiába történő transzformáció lehetőségét, azonban a csontvelő vizsgálata chronicus myeloid leukaemiát igazolt, típusos t(9;22) transzlokációval. A korábban igazolt 5q– klón nem volt kimutatható. Nilotinib kezelés indult 2 × 300 mg/nap dózisban és a lenalidomid terápia leállt. Kilenc hónap elteltével ismételten pancytopenia jelentkezett, mely miatt a nilotinib dózisát 2 × 150 mg-ra redukáltuk, és ellenőrző vizsgálatokat végeztünk, ami a CML major molekuláris remissziója mellett az 5q– MDS klón ismételt megjelenését igazolta. Kezelését a redukált dózisú nilotinib mellett a korábban effektív lenalidomid terápiával egészítettük ki, azonban alig két hónap után ismételten jelentős leukocytosist észleltünk, melynek hátterében az MDS acut leukaemiás transzformációja igazolódott. Azacitidine+venetoclax kezelést indítottunk a redukált nilotinib mellett, az első ciklus után végzett ellenőrző vizsgálat jó terápiás választ igazolt, azonban a beteg a második kezelési ciklus során kialakult lázas neutropeniás szövődményben meghalt. Betegünk esetét ritkasága és a diagnosztikus kihívások miatt tartjuk érdemesnek bemutatásra.

Open access