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  • Author or Editor: Kumar Raj x
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Molecular detection of Cucumber mosaic virus in various chrysanthemum cultivars was attempted by RT-PCR and Southern hybridization tests using CMV specific primers and cloned probes, respectively. A pair of primers was designed from a conserved region of the Cucumber mosaic virus coat protein (CMV-CP) gene, capable of amplifying a product of ~650 bp from various CMV strains. RT-PCR using the total nucleic acid from infected leaf samples and the specific primers resulted in positive amplification of an expected size band of ~650 bp in most of the samples. The identity of the PCR amplicons was checked by Southern hybridization using the a32P-labelled DNA probes prepared from the cloned coat protein gene of a well-identified strain of CMV isolated from Amaranthus. Positive signal of hybridization of PCR products and CMV probes confirmed the identity of fected chrysanthemum samples.

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Abstract

Daffodil (Narcissus tazetta L.) is a bulbous ornamental plant which belongs to the family Amaryllidaceae. It is popular for sweet-smelling beautiful flowers and is used for the fragrant oil and perfumes. It is also a source of alkaloids used in traditional medicines. Daffodil is commercially important ornamental plant, therefore, it is important to maintain its quality and production. Various type of viruses affect daffodil cultivation and among them, potyviruses are of great concern as they cause more economic losses to its commercial cultivation in term of reduction in bulb size, number of bulbs and the quality of daffodil flowers. In this review, a general overview of daffodils, genus Potyvirus, disease symptoms in daffodils linked to potyvirus infection, potyviruses described in daffodils, Potyvirus identification methods and possible management strategies of potyviruses in daffodil cultivation are described in detail. The study will be helpful to daffodil growers for improvement of the production/yield and quality of daffodil crop.

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Gerbera (Gerbera jamesonii) is a popular ornamental plant cultivated all over the world. It is grown in beds, pots and used as cut-flower in making bouquets and for decoration in ceremonial functions. Gerbera has been commercially cultivated by a large number of growers in India as a primary source of income, therefore, has high socioeconomic impact in floriculture industry. The gerbera cultivation areas in India are increasing day by day due to its uses and the market demands. Its cultivation has been hampered by a variety of diseases that affect its flower quality and quantity. Of them, the viral and phytoplasma diseases cause considerable losses in gerbera cultivation. In this review, we have described about the disease symptoms, detection methods and identification of causal virus and phytoplasma pathogens affecting gerbera production worldwide and their disease management strategies opted by the researchers for production of pathogen-free plants.

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