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Suspensions of a bioluminescent (luxAB) transformant of Listeria monocytogenes in pH 7.0 phosphate buffer were pressurised and the effect of the pressure treatment was monitored by plate counting. When the bacteria were suspended in NaCl- and nisin-free buffer the number of colony forming units (CFU) decreased by 3 and 6 log cycles after 300 MPA for 10 and 30 min, respectively. Supplementing the plating medium with 5% NaCl did not influence the colony forming capacity of non-pressurised cells, however, CFU of residual populations after respective treatments of 300 MPa for 10 and 30 min were reduced by a further 2 and 3.5 log cycles in case of salt containing plates. Nisin-addition to the plating medium caused less than one log unit decrease in the CFU of the non-pressurised population. However, the CFU of 10 min-pressurised sample was 4 log cycles less in the nisin-containing plates than in the nisin-free ones, whereas no colonies were formed in the nisin-containing plates even when 1 ml was inoculated from the originally 1010 CFU/ml population after 300 MPa for 30 min. The luciferase activities (bioluminescence intensities) decreased concomitant with the reduction of the viable cell counts, however, they were approx. 0.6-0.8 log units less in the presence of 5% NaCl in the pressurised suspension than those expected from the previously established linear correlation between the logarithmic light outputs and the logarithmic viable cell counts.

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Acta Veterinaria Hungarica
Authors:
Klára Oppel
,
L. Bárdos
,
A. Ferencz
,
Hajnalka Lakner
,
Judit Simon
,
Kriszta Temesváry
,
Krisztina Karchesz
, and
Margit Kulcsár

Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 µL samples and 200 µL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year. SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated ‘micro’ method as well as by the manual test tube ‘macro’ method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p < 0.001).

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Acute, severe hypovolemia is a medical emergency. Traditional vital sign parameters allow no optimal triage. High predictive power of finger plethysmography-based stroke volume (SV) and pulse pressure (PP) was recently suggested. To assess the performance of the PP and SV parameters, lower body negative pressure of −40 mmHg, than −60 mmHg — corresponding to moderate and severe central hypovolemia — was applied in 22 healthy males (age 35 ± 7 years). Slow breathing induced fluctuations in the above indices, characterized by stroke volume variability (SVV), and pulse pressure variability (PPV), were assessed. Responses in heart rate (HR) and shock index (SI) were also studied. Discriminative capacity of these parameters was characterized by the area under the ROC (receiver operating characteristic) curves (AUC).

Results

In comparison of baseline to severe central hypovolemia SV, PP, HR and SI showed good discriminating capacity (AUC 99%, 88%, 87% and 93%, respectively). The discriminating capacity of SVV and PPV was poor (77% and 70%, respectively). In comparison of moderate and severe hypovolemia, the discriminating capacity of the studied parameters was uniformly limited.

Conclusions

Plethysmography-based SV and PP parameters can be used to detect acute severe volume loss. Sensitive parameters discriminating moderate and severe central hypovolemia are still lacking.

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Purpose

Reduced functional mobility is a risk factor for falls. The Timed Up and Go test is a complex measurement tool for functional mobility. Our aims were to assess the functional mobility of: (a) community-living elderly who were participating in an exercise programme (n = 40; mean age = 73.7 years), (b) community-living elderly who were physically inactive (n = 40; mean age = 74.1 years), and (c) institutionalized elderly (n = 40; mean age = 73.5 years) and to compare the results with cut-off values for risk of fall.

Materials and methods

After measuring functional mobility, one-way independent ANOVAs and sample t-tests were used for analysis.

Results

The functional mobility of the active participants was better than that of the inactive (p < .001) and institutionalized participants (p < .001). There was no significant difference between the inactive and institutionalized participants (p = .990). The functional mobility of the active participants was better, whereas the functional mobility of the inactive participants was worse than the cut-off value of 13.5 s for risk of fall for community-living elderly. The functional mobility of the institutionalized participants did not differ from the 15-s reference value for predicting risk of fall.

Conclusion

The results indicate that regular physical activity has a positive effect on maintaining functional mobility among both community-living and institutionalized elderly individuals.

Open access

Factors associated with postural control in nursing home residents

Oral presentation at the 13th Conference of the Hungarian Medical Association of America – Hungary Chapter (HMAA-HC) at 30–31 August 2019, in Balatonfüred, Hungary

Developments in Health Sciences
Authors:
R.L. ErdŐs
,
I. Jónásné Sztruhár
,
A. Simon
, and
É. Kovács

Abstract

Purpose

Decline of the sensory and motor systems in older people negatively affects postural control. This increases the risk of falls, which is dangerous for older people in long-term care. Being aware of the quality of postural control and the factors affecting it among elderly people, is crucial in implementing an effective fall-prevention program. This study aimed to measure postural control and the demographic, health-related, and functional factors presumed to be correlated with it among nursing home residents. Another aim was to find valid screening tools based on these factors.

Materials and methods

Seventy one nursing home residents were included. Postural control was measured using the Berg Balance Scale. Grip strength, the 30-s chair stand test, and the Timed Up and Go test were used to measure global muscle strength, and functional mobility, respectively. The results of these functional tests were dichotomized using age-specific reference values.

Results

Postural control was significantly worse in those who did not reach the age-specific reference values in any of the three functional tests. Effect sizes were large for functional mobility and medium for muscle strength. Multimorbidity and gender had no effect on postural control in our sample.

Conclusions

Among nursing home residents, postural control is related to functional mobility and muscle strength. Thus, routine testing of these skills among elderly people is an important task of the physiotherapist.

Open access
Acta Physiologica Hungarica
Authors:
Teodora Mocan
,
S. Clichici
,
L. Agoşton-Coldea
,
L. Mocan
,
Ş Şimon
,
I. Ilie
,
A. Biriş
, and
Adriana Mureşan

Nanosized particles (NPs) have recently been proposed for extensive use, including into the biomedical field. As a result, research on toxicity and oxidative stress concerning the interaction of nanoparticle and the living organism has attracted increasing interest among specialists. Two different targets have been the motor of this type of research: 1) the safety concern regarding such NPs large-scale use along with the need to generate antidote solutions to possible adverse effects, 2) the idea of influencing oxidative damage and of using them for elaborating anticancer/antimicrobial therapies. Present study reviews recent research achievements within the proposed theme taking into account the nature and particularities of each type of nanoparticle.

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