Authors:A. Bagdi, G. Balázs, J. Schmidt, M. Szatmári, R. Schoenlechner, E. Berghofer, and S. Tömösközia
Six varieties of proso millet (Panicum miliaceum L.) and two commercially available millets were investigated in the present study. In order to explore the nutritional potential, major nutrient composition, mineral composition, antioxidant capacity, total phenols content (related to the antioxidant capacity) and dietary fibre content were determined. The effects of decortication on these components were examined. In addition, protein profile of the varieties and amylose/amylopectin ratio of the starch were examined. The range of the values measured for major nutrient composition corresponds with data of other millet species published in earlier studies. Remarkable differences were found among the protein contents of the varieties (11.58–14.80%). Although the concentration of minerals was low in the varieties examined, in comparison with other cereals wholegrain millet seems to be nutritionally valuable because of their high dietary fibre content. Decortication had no effect on the protein and fat content of millets, however, it significantly decreased the content of crude fibre, dietary fibre, minerals, total phenols content and antioxidant capacity. Consequently the applicability of millets as functional food decreases. Decortication had no effect on the amylose/amylopectin ratio of millet. No varietal differences were found in terms of protein characteristics.
Authors:A. A. Czelleng, Z. Bozsó, P. G. Ott, E. Besenyei, G. J. Varga, Á. Szatmári, Y. M. Hafez, and Z. Klement
Pseudomonas viridiflava is an opportunistic, post-harvest pathogenic bacterium that causes soft rot of fruits and vegetables. In vivo expression technology was used to identify genes that participate in the pathogenicity of P. viridiflava. Genetic loci that are induced in planta were identified. Ten such loci were partially sequenced and annotated. Here we describe five of them, which influence the pathogen's stress tolerance in planta. Three of the identified ORFs that show sequence identity to known genes encode membrane proteins, the remaining two encode enzymes in catabolic pathways.
Authors:R. Kiss, G. Szita, M. Herpay, Gy. Csikó, J. Pászti, T. Mag, P. Kovács, G. Kovács, J. Szita, P. Tóth, I. Szatmári, and S. Bernáth
The authors investigated the possibility of the presence of VTEC strains in improperly pasteurized milk samples. A total of 64 Escherichia coli strains were isolated from 135 pasteurized milk samples originating from the same producer. The examined isolates contained 29 haemolysin-, 9 colicin- and 5 aerobactin-producing strains, but the investigations concerning heat-resistant and heat-sensitive toxins gave negative results.Six O128-type E. coli strains exerted a cytotoxic effect on the VERO cell line; 5 of them contained H12 antigen, while one could not be typed. Four of the 6 verocytotoxin-producing strains belonged in phage group 20, one in phage group (2)3(7), and one in phage group 4; four strains were of B3, one of A1, and one of A1(A2) phage type.Because of a technical failure the milk was pasteurized at 69 °C for 15 s, which is 2 °C less than required. The results underline the importance of the appropriate pasteurization temperature, as otherwise the milk may contain verocytotoxin-producing E. coli, which is a potentially great hazard for public health.