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  • Author or Editor: Nancy Pandita x
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The γ-amino butyric acid (GABA) is the main inhibitory neurotransmitter system in the brain. Either imbalance in the extreme can result in several mental diseases like schizophrenia, cerebral stroke, temporal lobe epilepsy (TLE), Parkinson’s disease (PD), Huntington’s disease (HD), and anxiety disorders even death. Measurement of GABA in tissue will help to elucidate the metabolic role and diagnostic value. Various analytical techniques are employed to estimate GABA in biological samples but the experimental procedure and tedious techniques are required. The present study demonstrates a simple, feasible, and cost-effective high-performance thinlayer chromatography (HPTLC) method for estimation of GABA in mice brain tissue. The method was validated in terms of specificity, linearity, and detection limit. The precision was done for inter-day, intra-day, instrumental, and reproducibility. Accuracy was checked by recovery study. The results indicate that this method is fast, sensitive, and suitable for quantitative assessment.

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Enicostemma littorale (Chota-Chirayata or Mamejava) is a glabrous perennial herb belonging to the family Gentianaceae. This plant has been widely used in traditional system of medicines for the treatment of diabetes, malaria, and fever. Its anti-inflammatory, antioxidant, hypoglycemic, hepatoprotective, antihyperlipidaemic antiedematogenic, and anticancer activities have been reported. Ahigh-performance thin-layer chromatography (HPTLC) method has therefore been established for the simultaneous quantification of three biomarkers, namely, isoswertisin-5-O-β-d-glucoside, swertiamarin, and swertisin in the powder of E. littorale Blume. Chromatographic separation was performed on silica gel plates with ethyl acetatemethanol-water 8.0:1.0:0.5 (ν/ν) as mobile phase. Detection and quantitation were performed by densitometry with a deuterium lamp at 287 nm. The responses to reference standards were linear in the concentration range 25–75 μg mL−1 for isoswertisin-5-O-β-d-glucoside, 200–600 μg mL−1 for swertiamarin, and 100–300 μg mL−1 for swertisin. The relative standard deviation (RSD) for instrumental precision, intra-assay precision, and intermediate precision was <2%. Swertiamarin was present at high concentration and isoswertisin-5-O-β-d-glucoside at low concentration in the whole-plant powder. The accuracy of the method was determined by the measurement of recovery at three different concentrations. The average recovery was 99.88% for isoswertisin-5-O-β-d-glucoside, 99.64% for swertiamarin, and 99.10% for swertisin. The proposed HPTLC method was found to be simple, precise, selective, sensitive, and accurate for routine quality control of the whole-plant powder of E. littorale Blume.

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A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for quantification of isovitexin in dried whole-plant powder of Enicostemma littorale Blume. Chromatographic separation was performed on silica gel plates with acetonitrile-water, 6.0:4.0 (ν/ν), as mobile phase. Detection and quantitation were performed by densitometry, with a deuterium lamp, at 350 nm. The response to isovitexin reference standard was linear in the concentration range of 100–400 ng per band. The method was validated for precision and accuracy. The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was <2%. The percentage recovery was 99.76. This method can be used for routine quality control analysis of the whole-plant powder of E. littorale Blume.

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A simple, accurate and precise high-performance thin-layer chromatography (HPTLC) method was developed and validated for the simultaneous determination of quercetin and kaempferol in the methanolic extract of leaves of Centella asiatica.Leaves of two different chemotypes of Centella asiatica were obtained from two different regions viz. Maharashtra and Gujarat state, and a comparative study was performed for the presence of both the standards — quercetin and kaempferol.The HPTLC of flavonoids was performed on F254 TLC plates with toluene-ethyl acetate-chloroform-formic acid (6:4:4:1, ν/ν) as a mobile phase. Densitometric determination of flavonoids was performed at λ = 240 nm in reflectance/absorbance mode. The linear regression analysis data for the calibration plots showed a good linear relationship in the concentration range of 100–1000 ng/band with r 2 = 0.9764 and 0.9823 for quercetin and kaempferol, respectively. The accuracy of the method was confirmed by conducting recovery studies at three different levels using the standard addition method. The average recovery for quercetin was 98.60% and for kaempferol was 90.08%. The proposed HPTLC method provides good resolution of quercetin and kaempferol from other constituents present in methanolic extract of leaves of Centella asiatica and can be used for quantification of quercetin and kaempferol present in the extract. Statistical analysis of the data showed that the method is sensitive and selective for determination of flavonoids.

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Sida spinosa Linn. (synonyms: S. alba L., S. angustifolia Lam., S. angustifolia Mill.), Malvaceae, is an annual erect, branched small perennial herb, which grows abundantly on cultivated fields, waste areas, road sides, and hotter parts of India. S. spinosa has been used in traditional medicine for a variety of therapeutic purposes as astringent, cooling stomachic and in nervous, urinary, and cardiac diseases. A high-performance thin-layer chromatography (HPTLC) method has therefore been established for quantification of kaempferol and apigenin in plant powder of S. spinosa Linn. Chromatographic separation was performed on silica gel plates with dichloromethane-methanol-formic acid 8.0:1.0:0.5 (v/v), as mobile phase. Detection and quantitation were performed by densitometry, with a deuterium lamp, at 340 nm. The responses to reference standards were linear in the concentration range 150–450 μg mL−1 for kaempferol and 50–150 μg mL−1 for apigenin. The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was <2%. Kaempferol was present at high concentration, and apigenin was present at low concentration in the whole plant powder. The accuracy of the method was determined by measurement of recovery at three different concentrations. Average recovery was 99.47% for kaempferol and 99.26% for apigenin.The proposed HPTLC method was found to be simple, precise, selective, sensitive, and accurate for routine quality control of whole-plant powder of S. spinosa Linn.

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A simple high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantification of penta-O-galloyl-β-d-glucose (PGG), tetra-O-galloyl-β-d-glucose (TGG), chebulinic acid, chebulagic acid, and gallic acid from different samples of Triphala churna: TC1, TC2, TC3, TC4, Hirda, Behda, Amla. The method was validated for precision (expressed as coefficient of variation, CV [%]), accuracy, sensitivity, and selectivity. The accuracy of the method was determined by measurement of the recovery at three different concentrations. Average recovery was 98.46% for PGG, 100.02% for TGG, 100.05% for chebulinic acid, 99.79% for chebulagic acid, and 101.15% for gallic acid from Triphala churna. The proposed HPTLC method was found to be simple, precise, selective, sensitive, and accurate for routine quality control of the different samples of Triphala churna containing hydrolyzable tannins.

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