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Bacillus subtilis natto is a potential source of fructooligosaccharides (FOS), which can be obtained by fermentation and may stimulate the growth of beneficial microorganisms in the colon representing a strategy to manipulate the intestinal microbiota acting as a prebiotic compound. The present study focuses on the ability of Lactobacillus ssp. strains to utilize FOS as a sole energy source. The results showed that FOS was equally good as glucose to provide energy source. The highest prebiotic activity score was obtained with Lactobacillus plantarum ATCC 14917 grown on FOS (0.526), followed by Lactobacillus casei (LC-1) (0.222). The lowest score was for Lactobacillus paracasei ATCC 27092 (−0.051). The results suggests that specific combinations of probiotic (L. plantarum ATCC 14917 and L. casei (LC-1)) and prebiotic (FOS) could be used as synbiotics in dairy and other foods.

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Acta Alimentaria
J.M. Garcia
S. M. Castro
R. Casquete
J. Silva
R. Queirós
J.A. Saraiva
, and
P. Teixeira

The effect of sequential treatments of pressure (50–150 MPa, 10 ºC, 5 min) and temperature (57 ºC, 15 min) on the survival and bacteriocin production of Pediococcus acidilactici HA-6111-2 cells in the exponential growth phase was assessed. The growth curves were fitted with the modified Gompertz model, and the estimated maximum specific growth rate was considered to be pressure dependent. A delay in the maximum value of bacteriocin production was registered for more severe pressure conditions, but it was found more notorious for pressure followed by temperature treatments. At lower pressure intensity treatment, regardless of the application order, there was an enhancement of bacteriocin production per cell when compared to the control while maintaining the maximum production value. Bacteriocin production after the treatments can be described by an exponential model.

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