Authors:E. Polyakov, I. Volkov, V. Surikov, and L. Perelyaeva
Using physicochemical methods we showed that continuous (15 days) exposure of monazite powder in humic acid (HA) solutions
with different acidity gives rise to one to two orders of magnitude growth in the concentration of the monazite’ p-, d- and
f-elements of Periodic Table (Mg, Al, Si, P, Pb, Ti, Bi, Sc, Ti-Zn, REE, Th, U). The growth in the elements concentration
in the humic solutions contacting monazite is shown to depend on the initial concentration of HA in the solutions, pH. It
is concluded that these factors should be taken into consideration when inorganic phosphates and alike phases are used as
a matrix for the radionuclide wastes solidification.
Authors:E. Sheval, Yu. Kazhura, Nina Poleshuk, Elena Lazareva, Elena Smirnova, Natalia Maximova, and V. Polyakov
The extensive use of herbicides in agriculture becomes an important factor in environmental pollution, especially in case of slowly degradable compounds. Some agents act on plants during a long period of time, even if a very low concentration of the herbicide remains in the soil. Here, we investigated the toxicological effect of a low concentration of dinitroaniline herbicide, trifluralin, on growing seedlings of
L. Trifluralin in concentration of 1 μg/ml inhibited root growth. The mitotic activity of meristematic cells was suppressed due to the retardation of metaphase progression — alteration that can be caused by cytoskeleton disorder. Using antibodies to α-tubulin, we investigated the distribution of microtubules in root meristem cells. During all stages of mitosis, the highly regular system of microtubular cytoskeleton observed in control cells was slightly disorganized. An examination of root structure using light and electron microscopy demonstrated that the cell walls did not form normally during cell division that led to the appearance of large multinucleated cells. Also, the premature (pathological) cell differentiation was induced by trifluralin. A part of differentiating cells showed intracellular structural changes that are consistent with programmed cell death. It seems that the development of alterations in trifluralin-treated roots was due to the microtubular cytoskeleton disorganization.