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- Author or Editor: M. Thakur x
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Abstract
A method has been developed for chemical control of a short-lived radiopharmaceutical,52Fe. The optimum conditions have been investigated for the simultaneous determination of microgram amounts of nickel, iron and chromium in an admixture. The method developed is applicable for the determination of 0.01 to 20 μg·ml−1 of nickel, 0.01 to 50 μg·ml−1 of iron and 0.05 to 50 μg·ml−1 of chromium. A study has been made of the mutual interference of these elements at different concentrations, and also of the interference by various other cations and anions. A list has been given of the other elements that can be analysed using the supporting electrolyte. A method of routine analysis is described.
The potential of pyridoxal-5-phosphate dependent tyrosine decarboxylase (E.C. 4.1.1.25) of Lactococcus lactis was explored for the biotransformation of L-tyrosine to tyramine. Maximum bioconversion of L-tyrosine to tyramine was achieved in tyramine production medium (pH −5.5) at 30 °C after 16 h of incubation with 0.2% L-tyrosine. The yield of tyramine was found to be 11.8 μg/mL by the growing cells of L. lactis at shake flask level. Growth medium and different physico-chemical parameters to maximize the biotransformation of L-tyrosine to tyramine were optimized and yielded 1.9-fold increased synthesis of tyramine.
Abstract
A rapid method for simultaneous determination of fluorine and chlorine in radioactive liquid wastes with ion chromatography after pyrohydrolysis separation was proposed for routine analysis. The elements were separated from radioactive liquid wastes by pyrohydrolysis and were subsequently determined with ion chromatograpy. Total time taken to determine these elements is about 45 min including 30 min for the pyrohydrolysis and 15 min for ion chromatography. The results of recovery tests ranged 95% or above. The limits of detection for F and Cl are 0.5 and 0.8 mg kg−1, respectively.
Summary
A rapid, simple and validated reversed-phase high-performance liquid chromatographic method has been developed for analysis of oxaprozin in pharmaceutical dosage forms. Oxaprozin was separated on an ODS analytical column with a 45:55 (v/v) mixture of acetonitrile and triethanolamine solution (5 mm, pH 3.5 ± 0.05, adjusted by addition of 85% phosphoric acid) as mobile phase at a flow rate of 2.0 mL min–1. The effluent was monitored by UV detection at 254 nm. Calibration plots were linear in the range 160 to 240 μg mL–1 and the LOD and LOQ were 14.26 and 41.21 μg mL–1, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine QC determination of oxaprozin in tablets.
