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Acta Microbiologica et Immunologica Hungarica
Authors:
M. Thakur
and
W. Azmi
The potential of pyridoxal-5-phosphate dependent tyrosine decarboxylase (E.C. 4.1.1.25) of Lactococcus lactis was explored for the biotransformation of L-tyrosine to tyramine. Maximum bioconversion of L-tyrosine to tyramine was achieved in tyramine production medium (pH −5.5) at 30 °C after 16 h of incubation with 0.2% L-tyrosine. The yield of tyramine was found to be 11.8 μg/mL by the growing cells of L. lactis at shake flask level. Growth medium and different physico-chemical parameters to maximize the biotransformation of L-tyrosine to tyramine were optimized and yielded 1.9-fold increased synthesis of tyramine.
