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). Nattokinase (NK), a serine protease secreted by Bacillus subtilis var. natto found in the Japanese traditional food natto ( Suzuki et al., 2022 ), possesses a variety of key beneficial effects on cardiovascular system due to its strong anti-thrombus and
they are more likely to have the enzymes activated, which facilitate the decomposition of dyes. Pure Bacterial Cultures Starting from the 1970s, Bacillus cereus, Bacillus subtilis, and Aeromonas hydrophila were
% purity). Then, the xanthohumol extracts as well as Xantho-Flav® were tested at different concentrations (i.e., 100 and 1,000 μM) against Bacillus subtilis under varying pH conditions applying the disc diffusion assay. Whereas B. subtilis growth was
, Bacillus subtilis; Gram (−) bacteria as Escherichia coli , Pseudomonas aeuroginosa . They were incubated at 35–37 °C for 24–48 h and yeast as Candida albicans incubated at 30 °C for 24–48 h and then the diameters of the inhibition zones were measured
activities of the complexes were determined against ATCC reference and clinical microbial strains, i.e. Gram-positive ( Staphylococcus aureus 0364 , Bacillus subtilis , Enterococcus faecalis ATCC 2921), Gram-negative ( Klebsiella pneumoniae 2968
the papaya plant with enzyme activity of 100,000 U g −1 . Neutral proteases (Neu) was derived from Bacillus subtilis with enzyme activity of 200,000 U g −1 (Novozymes Biotechnology Limited). Other reagents used were chemicals of analytical grade. 2
. 10826795 10.1099/00207713-50-1-127 [37] Wang LT , Lee FL , Tai CJ , Kasai H . Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group . Int J Syst Evol Microbiol 2007 ; 57
the scientific literature as biocontrol agents for these bacteria. Földes and co-workers (2000) isolated a Bacillus subtilis strain, which was able to inhibit the growth of L. monocytogenes and Staph. aureus to a high degree. In a publication of
dissolved in 0.2, 0.4, and 0.8 mg mL −1 in dimethyl sulfoxide (DMSO), and the antimicrobial activity investigations were performed by using the Hollow Agar Method ( Alan and Yılmaz, 2019 ). Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923
. , Cogliati , S. , Leñini , C. , Bartolini , M. , Grau , R. : Microbial flora, probiotics, Bacillus
