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participate in the study. We determined serum fetuin-A concentration by radial immunodiffusion, as described earlier [ 21 ]. Anti- Helicobacter IgG was determined by ELISA using the NovaLisa kit (NovaTec, Dietzenbach, Germany). Values ≤1.0 were
a freezer (−20 °C) for storage. The concentration of melatonin was determined by a universal Melatonin ELISA Kit (ab285251, Abcam plc, Cambridge, UK; analytical sensitivity 4.7 pg mL −1 ; range 7.8–500 pg mL −1 ; intra-assay precision <5%; inter
> G) and (Tru9I-rs757343 A > G), respectively Measurement of 25-(OH) D 3 The concentration of 25-(OH) D 3 was measured in the plasma of patients with MTC (n = 40) and those in the control group (n = 40) using an Elisa kit according to instructions by
antigen ELISA for Entamoeba did not reveal any parasites, a full blood count and serum C-reactive protein (CRP) were unremarkable. Colonoscopy demonstrated multiple ulcerations, predominantly in the coecum and ascending colon on a red and
Hepatitis E virus (HEV) strains are classified into 4 genotypes by nucleotide sequencing. Genotypes 3 and 4 infect humans and animals via HEV-contaminated food or water. HEV RNA was detected by PCR and antibodies were detected by ELISA. Since human studies showed that HEV IgG antibodies in sera can persist for extended periods, diagnosis of HEV infection in swine or humans is mainly based on serological detection using commercial ELISA kits. However, there is no supplemental method to verify ELISA results. Hence, we developed a novel method used for mutual correction of these common processes. Here, a modified stable HepG2 cell line was transfected with pcDNA3.1-ORF3 to express the swine HEV ORF3 protein. Based on this cell line, a novel immunoperoxidase monolayer assay (IPMA) was developed to detect antibodies against HEV. The results show that this method has good specificity, sensitivity and repeatability. When used to investigate 141 porcine serum samples, the IPMA had a coincidence rate of 92.2% with a commercial ELISA kit. The established IPMA described herein is valuable as a supplemental method to ELISA and can differentiate infections by HEV and other viruses.
Over the last few years several porcine epidemic diarrhoea (PED) outbreaks have been discovered in Europe including the first PED case in Slovenia in January 2015. The aim of this study was to determine when PED virus (PEDV) infection started in Slovenia. Serum samples collected between 2012 and 2016 were tested. Three hundred and seventy-five serum samples were collected from 132 Slovenian small, one-site pig farms. Samples were tested for PEDV antibodies utilising three different serological methods: commercially-available indirect ELISA, in-house blocking ELISA test and Immunoperoxidase Monolayer Assay (IPMA) test. One hundred and seventy (45.33%) tested samples were found positive by the commercially-available ELISA test kit, and 10 (5.68%) of these 170 samples found positive were positive by the in-house blocking ELISA. Only these 10 samples were collected from a farm where clinical signs of PED infection had been observed and PEDV was confirmed by RT-PCR methodology; the other 160 samples were collected randomly. Thirty-two samples with the highest S/P value obtained with the commercial ELISA were all negative with IPMA. Reasons for the high variance in the results obtained remain unclear; more research is required to ensure higher sensitivity and specificity in terms of PEDV antibody tests and other PED diagnostic methods.
FABP-9 was assessed by the Mybiosource ELISA test kit (cat no: MBS918033, sensitivity 0.047 ng/mL, reference range 0.188–12 ng/mL) obtained from MyBioSource, Inc., USA. The results were indicated as ng/mL and ng/mg protein. Total protein analysis was
measurement of IL-6 was performed in duplicate in the blood samples by using an enzyme immunoassay with Human Il-6 ELISA kit (Elabscience Biotechnology Inc. USA). The detection range was 7.81–500 pg/mL, with intra and interassay coefficients of variation below
enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions (Human Kisspeptin-10 Elisa Kit, standard curve range: 0.05 ng/mL-10 ng/mL, sensitivity: 0.02 ng/mL, intra-assay CV: 8%; interassay CV: 10%; catalog no. E3919Hu
Several grapevine viruses were reported in Algeria and especially in grapevine germplasm collection, therefore it is a great challenge to free these varieties from virus infection before any breeding programs. Our study focused on the development of chemotherapy on autochthonous varieties collected in the grapevine germplasm collection of ITAFV. All these varieties were tested by DAS-ELISA and the presence of GLRaV-3 and GFLV was confirmed in all used samples for the sanitation. After 8 weeks of shoot tips in vitro culture in a modified M S medium containing ribavirin, DAS-ELISA test revealed that GLRaV-3 was completely eliminated and GFLV to a significant rate.
