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incubation at 37 °C for 24 h, the turbidity (quantified) of the suspensions were read at 450 nm using an ELISA reader. The lowest concentration of antibiotic that inhibited the growth of the tested bacterial strain, i.e. the MIC was determined by comparing
basis of seasonality (August–November), acute neurologic clinical signs (less than 5 days), positive serum IgM ELISA test (IDEXX IgM WNV Ab Test, Hoofddorp, The Netherlands), and the absence of any WNV vaccination in the history. Only clinically healthy
destaining solution (ethanol/demineralised water/glacial acetic acid, 7.5/7.4/0.15 v/v/v) was applied for 10 min. The viability of the IPEC-J2 cells was measured at 540 nm using an ELISA Plate Reader (EZ Read Biochrom 400, Cambridge, UK). Experimental layout
anaplasmosis and Lyme borreliosis in Canada as determined by a point-of care enzyme-linked immunosorbent assay (ELISA) . Can. Vet. J. 56 , 575 – 580 . Sellon , D. C. and Long , M. T. ( 2014 ): Anaplasma phagocytophilum infection . In: Sellon , D
). ELISA For detecting the TNF-α, IL-6, IL-1β protein levels, ELISA kits (TNFα kit, ab100785; Abcam; IL-6 kit, ab100772; Abcam; IL-1β kit, ab100768; Abcam) were used. The production of TNF-α, IL-6, and IL-1β in the hippocampus tissue of rats
reader (Epoch Microplate Spectrophotometer, Bio Tek, USA). NO production was then calculated by comparing the OD values of the samples with the standard curve. ELISA assay Soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell
% ethanol per well to remove the adherent stain. TSB containing 1% glucose was used as a placebo. The ELISA reader was used to spectrophotometrically measure the stained adherent biofilm's optical density (OD) at a wavelength of 490 nm. According to the
nm was measured using an ELISA plate reader (Plate reader, model–A4, serial no.-1910, Das, Italy). The cut-off value (ODc) was calculated for each microtiter plate and results were interpreted according to the formula presented in previous studies. A
: Az eddigiekben összesen 48 MM beteg és 10 kontroll személy vérmintáját vizsgáltuk. Az MM-ben rutinszerűen vizsgált laboratóriumi paraméterek mellett ELISA-val PACAP-38 szintet, míg Luminex módszerrel számos gyulladásos mikrokönyezeti faktor (IFN-γ, IL
time of measurements. After collection of all samples, serum copeptin levels were measured using “Human Copeptin ELISA Kits” (Phoneix Pharmaceuticals, USA; catalog no.: EK-065-32; lot no.: 603.858)” in microplate readers (Bio-Tek Instruments ELx800, USA
