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], liquid chromatography–mass spectrometry (LC–MS) or liquid chromatography–tandem mass spectrometry LC–MS/MS [ 16 , 17 , 18 , 19 , 20 , 21 ], by ultra-performance liquid chromatography (UPLC) coupled to MS (UPLC–MS) [ 22 , 23 ], and high

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Acta Chromatographica
Authors:
Bingying Hu
,
Yingying Sun
,
Min Wang
,
Zhisheng He
,
Shanshan Chen
,
Dake Qi
,
Zhen Ge
,
Lingling Fan
,
Jingfang Chen
, and
Yang Wei

%. UPLC-MS/MS instruments and parameters An API 5500 Triple Quad UPLC-MS/MS System (AB SCIEX, USA) was employed for UPLC-MS/MS analysis. Waters ACQUICY BEH C18 (2.1 × 100 mm, 1.7 μm) was selected as analytical column in this

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The experiment was manipulated on an UPLC Acquity C 18 column (2.1 mm × 100 mm, 1.7 µm) maintaining at 40 °C. The mobile phases were consisted of solvent A (5 mmol/L ammonium acetate and 0.1% formic acid solution) and solvent B (acetonitrile) with a

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pressure, which provides the ultra-performance liquid chromatography (UPLC) separation on a conventional HPLC system [ 17 , 18 , 26 ]. Hence, combining the advantages of HPLC-MS and poroshell column would be a good strategy to develop a rapid analytical

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calculated in term of (mass of product)/(initial mass of vegetable matrix). The extraction products analysis UPLC PDA analyses were performed on Ultra Pressure Liquid Chromatographic Acquity system (UPLC, Acquity I-Class, Milan, Italy) consisting of a Waters

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(BPR). The Waters ACQUITY UPLC H-Class system is equipped with a quaternary solvent manager, an autosampler, a column manager, and a PDA detector. Data acquisition and control of the SFC and ultrahigh-performance liquid chromatography (UHPLC) system

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hospital ethical approval. Analytical instrumentation The analysis was performed using a ACQUITY UPLC H-Class System coupled with a XEVO TQD triple quadruple mass spectrometer (Waters Corp., Milford, MA, USA). The mass spectrometer was equipped with an

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substances, either in products for the treatment of erectile dysfunction or in dietary supplements for weight loss by an ultra-performance liquid chromatography (UPLC) technique [ 14 , 15 ], liquid chromatography–mass spectrometry [ 16 ], and high

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(Kala SG, et al., 2021) [ 9 ] HPLC No 10–2,000 35 100 2022 (Yang X, et al., 2022) [ 7 ] UPLC-MS/MS No 25–8,000 4 100 2023 (Fukuda N, et al., 2023) [ 10 ] HPLC No 10–5,000 15 100 2023 (Yasu T, et al., 2023) [ 11 ] HPLC No 0.25–10 22.3 50 2023 (De Gregori

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. The previously developed methods [ 5–11 ] provide assasys in bulk and pharmaceutical forms, they are not suitable for plasma assays and their sensitivity is not enough for bioanalytical studies. Other MS methods; LC-MS/MS [ 12 , 13 , 15–17 ] and UPLC

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