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Old-fields (44, aged 1–15 years, from Czech Republic and Hungary) were sorted according to their soil moisture and nitrogen content into wet, mesic or dry, and nutrient poor, moderate or nutrient rich categories, resulting in 8 combinations (dry and nutrient rich fields were not present). The vegetation of old fields was sampled using phytosociological relevès. The changes in species cover data and importance of species trait categories were analysed in relation to three environmental factors, i.e., time since abandonment, soil moisture and total soil nitrogen using ordination, generalized linear models (GLM) and regression tree methods. Successional seres in the first 15 years after field abandonment were divergent. Species diversity significantly decreased with increasing site moisture and was highest in sites with moderate nitrogen content; while the relationship with time was not significant. Raunkiaer life forms and life strategies (sensu Grime) were generally the most predictive species traits considering species occurrence during the course of succession, the type of dispersal considering the different moisture status, and the ability to lateral spread considering the nutrient status of the old-fields. Most trends appeared in both parametric GLM and non-parametric regression tree analyses, several only in GLM. We consider regression trees to be a more convenient tool than GLM in cases such as ours with a rather small number of samples and robust character of data. Another advantage is that a hierarchy of species traits is taken into account. Thus, the occurrence of a species along an environmental gradient can be predicted if the species possesses a certain combination of traits.

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Biome interfaces are expected to exhibit chorological symmetry, i.e., decreasing trends in the number of species associated with each of the two neighbouring biomes as we progress from one into the other. Our aim was to test for such a pattern within the forest steppe biome, which is a transition zone in itself between the temperate deciduous forests and the steppe biome. Presence of chorological symmetry would provide indirect evidence for the prehuman presence of zonal steppes in the Carpathian basin. We also whished to provide an example with this analysis for drawing biogeographical conclusions based on quantitative species occurrence data, an information source hitherto neglected in Central Europe. Occurrence patterns of forest and steppe species were analysed at the Duna-Tisza köze (Danube-Tisza Interfluve) by the traditional qualitative biogeographic method and by hierarchical classification of predicted spatial pattern based on Generalized Linear Models with logistic link function. Species presences were explained by variables describing spatial orientation. In this approach, an outgroup of sand grassland species was also added to characetrise the discrimination ability of the approach. The quantitative method discriminated the out-group of sand grassland species, providing evidence of its suitability for our purpose. The results of the quantitative investigations were also in accordance with the qualitative evaluation. Surprisingly, forest and steppe species showed similar distributional patterns, i.e., no chorological symmetry was discernable. The quantitative biogeographic approach unveiled important evidence for deciding about the potential presence of zonal steppes in the Carpathian basin. Although the observed similarity of the distribution of forest and steppe species may have multiple reasons, the major cause of the lack of chorological symmetry is most probably the lack of zonal steppe South of the forest steppe biome in the Carpathian basin. Additional explanations include land use pattern and the mountain belt around the basin acting as a refugium in the ice ages.

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Little is known of how changes in plant function may influence adaptive traits amongst animals further up the food chain. We addressed the hypothesis that shifts in plant functional traits are associated with the adaptive function of animal species which have an indirect trophic link. We compared community characteristics and functional traits of two trophically detached biotic groups (vascular plants and carabid beetles) along a primary succession on terrain at the Cedec glacier in the Alps, where deglaciation events following post-Little Ice Age climate warmings are marked by moraine ridges. Morphofunctional traits were recorded: canopy height (CH), leaf dry matter content (LDMC), leaf dry weight (LDW) and specific leaf area (SLA) (for plants) and the number of brachypterous, autumn-breeding and predator species, and average body length (for carabid beetles). We found that vegetation cover and plant species richness gradually increased throughout early succession, with an abrupt increase between 40 and 150 years after deglaciation. At the early stages of the succession plant traits were typical of ruderal species (high SLA, low CH, LDW) whilst a shift in traits towards stress-tolerance (low SLA) occurred >150 years. Carabid communities and traits changed alongside changes in plant species richness and cover, with late successional vegetation hosting larger, more diverse, less mobile carabid species with longer larval development. Thus, ruderal plant strategies are the main contributors during vegetation development, determining vegetation quantity, and probably have the greatest impact on changes in carabid assemblages by regulating resource availability. Plants then require greater stress-tolerance to survive in stable vegetation, which supports high carabid diversity. This suggests that different plant strategies may affect ground beetle communities via contrasting mechanisms: both quantities (biomass, species richness) and qualities (functional traits, adaptive strategies) should be taken into account during studies of plant-animal interactions within ecosystems.

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Different types of forest use significantly changed the structure and species composition of European temperate forests. Herbaceous species and seedlings are important parts of the forest ecosystem, thus it is necessary to understand the effects of stand characteristics on the species composition of the understory. In our study we assessed the main factors that affect the species composition of herb and tree seedling assemblages in Quercus petraea and Q. cerris dominated stands (age 50–150 years) in the Bükk Mountains, Hungary. The relationship between the studied assemblages and explanatory variables (tree species composition, stand structure, canopy closure and topography) were explored by Redundancy Analysis (RDA). The occurrence of herbaceous species was affected by canopy closure, stand structure (mean DBH and DBHcv of trees), topography and the density and diversity of shrub layers. Oak forest species were associated with more open stands with sparsely distributed large trees, while mesic forest species were positively associated with heterogeneous stand structure, low shrub density, and western exposure. Seedlings of trees and shrubs showed a dispersal limited phenomenon. The composition of seedlings was significantly influenced by the mean DBH of trees, the structural heterogeneity of the overstory, the tree species diversity and the density of shrub layers. However the seedlings of both dominant oak species required the same stand structure, sessile oak was able to regenerate almost exclusively in those stands where it was dominant in the overstory, which is significant for the management of the species. Generally, forest management affects species composition and structure of the overstory, accordingly it had direct and indirect effects on the understory community as well.

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429 439 Kaur-Sawhney, R., Shih, L. M., Flores, H. E., Galston, A. W. (1982) Relation of polyamine synthesis and titer to ageing and senescence in oat leaves. Plant Physiol. 69

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hippocampal neuronal loss in normal ageing and Alzheimer’s disease. Lancet 344, 769–772. Troncoso J. C. Differences in the pattern of hippocampal neuronal loss in normal ageing and

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Pentreath, V. W., Radoycic, T., Seal, L. H., Winstanley, E. K. (1985) The glial cells and glia-neuron relations in the buccal ganglia of Planorbis corneus (L.): cytological, qualitative and quantitative changes during growth and ageing. Phil. Trans. R

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, M. A. (2002) Modulation of age-related biochemical changes and oxidative stress by vitamin C and glutathione supplementation in old rats. Ann. Nutr. Metab. 46 , 165-168. Modulation of age-related biochemical changes and

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Baguszewski, P., Zagrodzka, J. (2002) emotional changes related to age in rats — a behavioral analysis. Behav. Brain Res. 133 , 323–332. Zagrodzka J

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101 215 220 Kaplan, D. T., Furman, M. I., Pincus, S. M., Ryan, S. M., Lipsitiz, L. A., Goldberger, A. L. (1991) Aging and complexity of

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