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A szerzők egy 46 éves fogva tartott férfi beteg esetét mutatják be, akinél Coxiella burnetii által okozott atípusos pneumoniát állapítottak meg. A mellkasröntgen-felvétel interstitialis pneumoniát mutatott ki. Western-blot és ELISA-tesztek Coxiella burnetii-antitestre pozitívak voltak. A beteg klinikai állapota 10 napos doxycyclin- és amoxicillin-, valamint B6-vitamin-kezelést követően javult. A szerzők kiemelik, hogy a Q-láz diagnózisának megállapítása specifikus antitestvizsgálaton alapul. Atípusos pneumonia esetén a hasonló tüneteket okozó egyéb baktérium, illetve vírusantitest-vizsgálatnak is fontos szerepe van. Orv. Hetil., 2015, 156(18), 741–743.
The expression levels of two marker proteins (phosphinotrichin acetyltransferase, PAT and wheat germ agglutinin, WGA) in the transgenic wheat lines and their resistance to digestion in small intestine of rats were studied in comparison with their non-transgenic counterpart obtained from green house and field experiments of two subsequent years. The marker proteins were quantified by ELISA. It was found that the expression of PAT and WGA markedly increased when the wheat was grown in the field compared to that in the greenhouse. There were no significant differences between the WGA contents of the parent and transgenic wheat lines, but a broad range of expression of PAT and WGA was observed in the transgenic lines. PAT did not survive in the small intestine of the rats, while WGA was resistant to digestion in different ratios and was bound to the intestinal epithelium.
Proteins of pea variety Kwestor, grown in Poland, were modified with acetic or succinic anhydride (0.01–1.0 g/g anhydride/protein). Degree of acylation, electrophoretic properties (SDS-PAGE) and immunoreactivity (by ELISA) were studied. The study indicates that not only the degree of acylation but also the type of anhydrides affected the extent of changes in the immunoreactivity of individual pea proteins. The greatest reductions in the immunoreactivity of albumins and legumin were observed during acylation with 0.2 g anhydrides (by 91–99% and 79–97% during succinylation and acetylation, respectively). The lowest immunoreactivity of vicilin fraction was found when 1.0 g of anhydride was used (about 6% during succinylation and 14.7% during acetylation as compared to the immunoreactivity of vicilin in native pea proteins).
The authors have developed transgenic wheat lines with broad range of herbicide resistance. The transgenic wheat, containing bacterial derived alien gene (bar) regulated under the maize ubiquitin promoter, is resistant to glyphosinate (phosphinotrichin) agent family. The presence of bar gene expression product (phosphinotrichin acetyl transpherase enzyme, PAT) was confirmed by PAT-specific ELISA (Enzyme Linked Immuno Sorbent Assay). The qualitative and quantitative chemical composition of the transgenic wheat lines in comparison with their non-transgenic counterpart (year 2000-2002) and protein utilization of the wheat wholemeal flours (year 2002) were summarized. Nutritional evaluation of the protein was based on a rat model by using N-balance experiments. Among the protein sources, heat-treated samples were also introduced into the experimental diets. It was found that heat denaturation of the proteins led to results with somewhat increased biological value indices. The introduction of GM technology did not affect food intake or nutritional performance of the rats.
Commercial sodium caseinate isolate (SCI) was hydrolysed with either protease Subtilisina carlsberg - Alcalase 2.4 FG (purchased from Novo Nordisk), pronase from Streptomyces griseus, and papain EC 3.4.22.2 (both from Sigma) in a two-step process to determine the changes in the immunoreactivity of a-, ß- and ?-casein. Enzymatic hydrolysis of SCI was performed by pH-stat method. Hydrolysates were analysed using IEF, SDS-PAGE, 2D electrophoresis, FPLC-gel permeation chromatography. Immunoreactive properties of peptide fractions separated from the hydrolysates by FPLC were determined using dot-immunobinding and ELISA methods. The two-step process was observed to be effective in reduction of casein fractions immunoreactivity, however, allergenic epitopes were still present in all peptide fractions.
Herpes simplex virus type 2 infection is a quite common but frequently asymptomatic, therefore undiagnosed condition. Genital HSV-2 infection may cause neonatal herpes, enhances HIV transmission and may play a role in infertility. To evaluate the prevalence of HSV-2 in Hungary we tested 2500 serum samples for the presence of anti-HSV-2 IgG by ELISA method. According to our results Hungary belongs to the low-infected countries, the HSV-2 seroprevalence grows with age and is significantly higher among women than in men. We also examined the serostatus of 512 pregnant women and 539 women attending infertility clinics. Results show that the HSV-2 prevalence is significantly higher among women attending infertility clinics and the seropositivity of pregnant women is similar to that of the general Hungarian women population with the same age.
The effect of germination conditions on the lectin of Lens culinaris var. Magda 20 seeds was studied. The seeds were germinated at 20 °C under different conditions of watering and light and for different periods of time. The seed lectin was assayed by haemagglutination and quantified by competitive ELISA. Changes in lectin content during germination were also monitored by SDS-PAGE and immunoblotting. Haemagglutinating activity and lectin content in the seeds were not changed during the first three days regardless of the conditions of the germination. However, lectin concentration was significantly higher after six days of germination; relative lectin levels being particularly high when germination was carried out in the light and with daily watering. The results of SDS-PAGE and immunoblotting have also shown that the lectin was not degraded during the first six days of germination however, other storage-proteins were broken down by proteolysis.
The deoxynivalenol content, presence of kernels infected by Fusarium spp. pathogens and the frequency of visually scabby kernels in wheat intended for human consumption were monitored in 2003–2005. About 1000 samples provided by growers from various regions of the Czech Republic were analysed each year. Immunochemical assay ELISA-based kits for the analysis of deoxynivalenol content were employed. In 2003, 2004 and 2005, deoxynivalenol was detected at the maximum levels of 5090 μg.kg −1 , 18300 μg.kg −1 and 4437 μg.kg −1 , respectively. Significant correlations were found between the deoxynivalenol content and percentage of kernels infected by Fusarium spp. pathogens, and percentage of visually scabby kernels. In all years under study, the relationship between the deoxynivalenol content and percentage of visually scabby kernels was closer than that between the deoxynivalenol content and percentage of kernels infected by Fusarium spp.
Maize dwarf mosaic is the most widespread virus disease affecting corn production in Hungary and Bulgaria. Samples from virus infected maize were collected from different part of Bulgaria and employed test plants, ELISA serological method and RT-PCR in order to identify the viral pathogen. Maize dwarf mosaic virus (MDMV) was detected in all tested samples. For further investigation three MDMV isolates were selected and cloned. Cloned cDNAs representing the coat protein gene of the virus have been sequenced. The coat protein genes of Bulgarian and Hungarian isolates of MDMV were compared. The nucleotide sequence identity and amino acid sequence similarity of the coat protein region varied from 88% to 99.1% and from 95.1% to 99.6%, respectively. The N-terminal region of coat protein was compared with other members SCMV subgroup and phylogenetic tree was constructed.
Abstract
Background: Amyotrophic lateral sclerosis (ALS) is a degenerative disease characterized by progressive depletion of motor neurons in brain, brain stem, and spinal cord, whereas Hirayama disease (HD) results from anterior horn cell loss in the lower cervical cord. On the other hand, matrix metalloproteinases (MMPs) are known to digest components of the extracellular matrix. Of these, MMP-9 is considered as a marker of neuroinflammation. Materials and methods: We have measured MMP-9 levels in the serum of 30 patients with ALS, 10 patients of HD, and 25 healthy controls using ELISA method. Results: MMP-9 levels were significantly elevated in the patients suffering from ALS (P < 0.01) and HD (P < 0.05). Furthermore, MMP-9 levels in ALS have a significant positive correlation (R 2 = 0.9) with the duration of the disease. Conclusions: These results suggest that neuroinflammation is an underlying component in ALS pathology and further opens up the search for suitable biomarkers.
