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. The first gel to show a protein pattern was destained with 50% methanol for in-gel extraction of immunoreactive spots numbered 1 to 4, for proteomic analysis by nanoscale LC coupled to tandem mass spectrometry (nano LC-MS/MS) (Thermo Electron, San Jose

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., Neubauer, P. & Hohtola, A. (2004): LC/MS/MS identification of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggregates. Biomed. Chromat. , 18 , 550–558. Hohtola A

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.4–6.3 [ 15 ] Ligustrazine Methanol solution (80%) 1 g 10 mL Methanol – Electrochemical Sensor 0.1 μM – 99.2–101.3 1.8–3.6 [ 13 ] Ligustrazine Rat plasma 100 μL 200 μL Acetonitrile: methanol (1:1, v/v ) Automated on-line SPE LC-MS/MS 0.20 – 90.5–90.8 7

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Acta Chromatographica
Authors:
Haiya Wu
,
Mengrou Lu
,
Jiamin He
,
Miaoling Huang
,
Aote Zheng
,
Meiling Zhang
,
Congcong Wen
, and
Jufen Ye

chromatography–tandem mass spectrometer (LC–MS/MS), ultra-performance LC–MS/MS (UPLC–MS/MS) has higher detection sensitivity with more powerful separation and analytical capability, which are essential for studying Chinese medicine with complex contents and the

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. Lc–Ms–Ms simultaneous determination of paracetamol, pseudoephedrine and chlorpheniramine in human plasma: application to a pharmacokinetic study . Chromatographia 2008 , 67 , 687 – 694 . 10

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(DLLME) method for the rapid determination of drugs of abuse, benzodiazepines and other psychotropic medications in blood samples by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and application to forensic cases

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been considered as a method of “gold standard” for quantitative food testing. When compared to traditional liquid chromatography, LC-MS/MS methods give greater mass measurement accuracy, better peak separation, and high accuracy and resolution. It also

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Acta Chromatographica
Authors:
Filip Šibul
,
Dejan Orčić
,
Sanja Berežni
,
Goran Anačkov
, and
Neda Mimica-Dukić

investigated compounds, SSE was close to 1 (with a deviation less than ±0.25) in a wide concentration range. Identification and Quantification of Phenolic Compounds (LC–MS/MS Profiling) Extraction, separation, identification

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supernatant was then filtered through an SPE column. This filtrate was collected and passed through a 0.22 μm membrane in preparation for mass spectrometry analysis. LC-MS/MS analysis Sample analysis was performed using a 2040C HPLC (Shimadzu, Japan) coupled

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analytical methods described for the determination of simvastatin in aqueous samples and human plasma including liquid chromatography (LC) [ 10 – 12 ], liquid chromatography–tandem mass spectrometry (LC–MS/MS) [ 13 – 16 ], gas chromatography–mass spectrometry

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