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as described previously ( Korczak et al., 2014 ). Sequencing of PCR products was performed by Macrogen Europe (Amsterdam, The Netherlands). Nucleotide sequences were aligned and compared using Geneious Prime software (version 2019.2.1; http

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Epstein–Barr-vírus által indukált hemolízis diffúz nagy B-sejtes limfómában

Epstein-Barr-Virus Induced Hemolysis in Diffuse Large B Cell Lymphoma

Hematológia–Transzfuziológia
Authors:
Réka Németh
,
Réka Ráhel Bicskó
,
Árpád Illés
, and
Lajos Gergely

etiológiájaként PCR-vizsgálattal megerősítve Epstein–Barr-vírus fertőzés igazolódott (gyengén pozitív, <249 kópia/ml), amelyet – tekintettel a beteg korábbi EBV IgG pozitivitására – reaktivációnak tekintettünk, újabb EBV szerológiai vizsgálatot nem végeztünk

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Acta Microbiologica et Immunologica Hungarica
Authors:
Raina Gergova
,
Virna-Maria Tsitou
,
Svetoslav G. Dimov
,
Lyudmila Boyanova
,
Kalina Mihova
,
Tanya Strateva
,
Ivanka Gergova
, and
Rumyana Markovska

extraction kit (AmpliSens, Moscow, Russia), in accordance with the manufacturer's instructions. All DNA extractions were stored at −70 °С until testing. All phenotypically identified S. aureus strains were PCR confirmed using species-specific primers

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Acta Microbiologica et Immunologica Hungarica
Authors:
Tran Duc Anh Ly
,
Linda Hadjadj
,
Van Thuan Hoang
,
Ndiaw Goumbala
,
Thi Loi Dao
,
Sekene Badiaga
,
Herve Tissot-Dupont
,
Philippe Brouqui
,
Didier Raoult
,
Jean-Marc Rolain
, and
Philippe Gautret

described [ 18 ] using a BioRobot®EZ1 Advanced XL instrument (QIAGEN, Hilden, Germany) and DNeasy® Blood & Tissue according to the manufacturer's instructions. The DNA extraction quality was assessed by RT-PCR targeting internal control TISS phage that was

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Acta Microbiologica et Immunologica Hungarica
Authors:
Seyedeh Marzieh Jabbari Shiadeh
,
Leila Azimi
,
Taher Azimi
,
Ali Pourmohammad
,
Mehdi Goudarzi
,
Bahare Gholami Chaboki
, and
Ali Hashemi

Concentrations (MICs) of different antimicrobials and the expression of efrAB genes in ciprofloxacin resistant E.faecalis strains by quantitative Real-Time PCR (qRT-PCR). Finally, we chose more resistant isolates for typing by Multilocus Sequence Typing (MLST

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:20,000 Chlamydia sp. monoclonal, mouse Progen, Heidelberg, Germany 1:100 Toxoplasma gondii polyclonal, rabbit NeoMarkers, Fremont, CA, USA 1:1,000 Polymerase chain reaction (PCR) In the case of Mycoplasma , previous investigations ( Wöhrer et al., 2016

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CMV-infekció autológ őssejt-transzplantált betegekben. Surveillance-vizsgálat vagy tünetek vezérelte diagnosztika szükséges?

Cytomegalovirus infection in patients after autologous stem cell transplantation. Should surveillance or a diagnostic-driven approach be preferred?

Hematológia–Transzfuziológia
Authors:
Klára Piukovics
,
Flóra Tímár Pásztor
,
Zita Borbényi
,
Edit Urbán
, and
Gabriella Terhes

stem cell transplantation detected by multiplex PCR assay. J Med Virol. 2017; 89: 358–362. 9 Ljungman P, de la Camara R, Cordonnier C, et al. Management of CMV, HHV-6, HHV-7 and

Open access
Acta Microbiologica et Immunologica Hungarica
Authors:
Arash Abednezhad
,
Bita Bakhshi
,
Nastaran Asghari Moghadam
,
Nima Faraji
,
Elahe Derakhshan-Nezhad
, and
Hajar Mohammadi Barzelighi

was confirmed by PCR amplification using 16srRNA specific primers (16srDNA-F, 5′ -GGGGGATCTTCGGACCTCA-3′; and 16srDNA-R, 5′ - TCCTTAGAGTGCCCACCCG-3′) [ 13 ]. Genomic DNA was extracted by boiling method and subjected to PCR amplification under the

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biochemical tests, and amplification 16S rRNA and bla OXA-51-like carbapenemase genes by the polymerase chain reaction (PCR) using primers, as listed in Table 1 [ 14 ]. Table 1

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Acta Veterinaria Hungarica
Authors:
Juan Aguilar-Marín
,
Carlos Cruz-Vázquez
,
Irene Vitela-Mendoza
,
Leticia Medina-Esparza
,
Isabel De Velasco-Reyes
, and
Miguel Ramos-Parra

aseptic conditions using the commercial kit Quick-DNA Miniprep Plus Kit (Zymo Research, USA), following the manufacturer's instructions. The parasite DNA was detected with a PCR test to amplify an approximately 450 bp fragment of the 529 bp repetitive

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