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as described previously ( Korczak et al., 2014 ). Sequencing of PCR products was performed by Macrogen Europe (Amsterdam, The Netherlands). Nucleotide sequences were aligned and compared using Geneious Prime software (version 2019.2.1; http
Epstein–Barr-vírus által indukált hemolízis diffúz nagy B-sejtes limfómában
Epstein-Barr-Virus Induced Hemolysis in Diffuse Large B Cell Lymphoma
etiológiájaként PCR-vizsgálattal megerősítve Epstein–Barr-vírus fertőzés igazolódott (gyengén pozitív, <249 kópia/ml), amelyet – tekintettel a beteg korábbi EBV IgG pozitivitására – reaktivációnak tekintettünk, újabb EBV szerológiai vizsgálatot nem végeztünk
extraction kit (AmpliSens, Moscow, Russia), in accordance with the manufacturer's instructions. All DNA extractions were stored at −70 °С until testing. All phenotypically identified S. aureus strains were PCR confirmed using species-specific primers
described [ 18 ] using a BioRobot®EZ1 Advanced XL instrument (QIAGEN, Hilden, Germany) and DNeasy® Blood & Tissue according to the manufacturer's instructions. The DNA extraction quality was assessed by RT-PCR targeting internal control TISS phage that was
Concentrations (MICs) of different antimicrobials and the expression of efrAB genes in ciprofloxacin resistant E.faecalis strains by quantitative Real-Time PCR (qRT-PCR). Finally, we chose more resistant isolates for typing by Multilocus Sequence Typing (MLST
:20,000 Chlamydia sp. monoclonal, mouse Progen, Heidelberg, Germany 1:100 Toxoplasma gondii polyclonal, rabbit NeoMarkers, Fremont, CA, USA 1:1,000 Polymerase chain reaction (PCR) In the case of Mycoplasma , previous investigations ( Wöhrer et al., 2016
CMV-infekció autológ őssejt-transzplantált betegekben. Surveillance-vizsgálat vagy tünetek vezérelte diagnosztika szükséges?
Cytomegalovirus infection in patients after autologous stem cell transplantation. Should surveillance or a diagnostic-driven approach be preferred?
stem cell transplantation detected by multiplex PCR assay. J Med Virol. 2017; 89: 358–362. 9 Ljungman P, de la Camara R, Cordonnier C, et al. Management of CMV, HHV-6, HHV-7 and
was confirmed by PCR amplification using 16srRNA specific primers (16srDNA-F, 5′ -GGGGGATCTTCGGACCTCA-3′; and 16srDNA-R, 5′ - TCCTTAGAGTGCCCACCCG-3′) [ 13 ]. Genomic DNA was extracted by boiling method and subjected to PCR amplification under the
biochemical tests, and amplification 16S rRNA and bla OXA-51-like carbapenemase genes by the polymerase chain reaction (PCR) using primers, as listed in Table 1 [ 14 ]. Table 1
aseptic conditions using the commercial kit Quick-DNA Miniprep Plus Kit (Zymo Research, USA), following the manufacturer's instructions. The parasite DNA was detected with a PCR test to amplify an approximately 450 bp fragment of the 529 bp repetitive