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extraction kit (AmpliSens, Moscow, Russia), in accordance with the manufacturer's instructions. All DNA extractions were stored at −70 °С until testing. All phenotypically identified S. aureus strains were PCR confirmed using species-specific primers
Concentrations (MICs) of different antimicrobials and the expression of efrAB genes in ciprofloxacin resistant E.faecalis strains by quantitative Real-Time PCR (qRT-PCR). Finally, we chose more resistant isolates for typing by Multilocus Sequence Typing (MLST
described [ 18 ] using a BioRobot®EZ1 Advanced XL instrument (QIAGEN, Hilden, Germany) and DNeasy® Blood & Tissue according to the manufacturer's instructions. The DNA extraction quality was assessed by RT-PCR targeting internal control TISS phage that was
:20,000 Chlamydia sp. monoclonal, mouse Progen, Heidelberg, Germany 1:100 Toxoplasma gondii polyclonal, rabbit NeoMarkers, Fremont, CA, USA 1:1,000 Polymerase chain reaction (PCR) In the case of Mycoplasma , previous investigations ( Wöhrer et al., 2016
CMV-infekció autológ őssejt-transzplantált betegekben. Surveillance-vizsgálat vagy tünetek vezérelte diagnosztika szükséges?
Cytomegalovirus infection in patients after autologous stem cell transplantation. Should surveillance or a diagnostic-driven approach be preferred?
stem cell transplantation detected by multiplex PCR assay. J Med Virol. 2017; 89: 358–362. 9 Ljungman P, de la Camara R, Cordonnier C, et al. Management of CMV, HHV-6, HHV-7 and
was confirmed by PCR amplification using 16srRNA specific primers (16srDNA-F, 5′ -GGGGGATCTTCGGACCTCA-3′; and 16srDNA-R, 5′ - TCCTTAGAGTGCCCACCCG-3′) [ 13 ]. Genomic DNA was extracted by boiling method and subjected to PCR amplification under the
biochemical tests, and amplification 16S rRNA and bla OXA-51-like carbapenemase genes by the polymerase chain reaction (PCR) using primers, as listed in Table 1 [ 14 ]. Table 1
aseptic conditions using the commercial kit Quick-DNA Miniprep Plus Kit (Zymo Research, USA), following the manufacturer's instructions. The parasite DNA was detected with a PCR test to amplify an approximately 450 bp fragment of the 529 bp repetitive
concentration by flame atomic absorption spectroscopy. The hepatic copper concentration of the fetus was 68.8 mg kg −1 . For molecular analysis, refrigerated brain and cerebrospinal fluid were submitted to a polymerase chain reaction (PCR) for
reservoirs of this pathogen. Until now, only little information has been available regarding the prevalence of A. phagocytophilum in horses. For example, Slivinska et al. (2016) tested 39 horses from Slovakia by PCR and observed only one positive case
