Search Results

You are looking at 131 - 140 of 885 items for :

  • Medical and Health Sciences x
  • Refine by Access: All Content x
Clear All
Acta Veterinaria Hungarica
Authors:
Monika Drážovská
,
Boris Vojtek
,
Jana Mojžišová
,
Simona Koleničová
,
Filip Koľvek
,
Marián Prokeš
,
Ľuboš Korytár
,
Alexander Csanady
,
Anna Ondrejková
,
Tatiana Vataščinová
, and
Mangesh Ramesh Bhide

reservoirs of this pathogen. Until now, only little information has been available regarding the prevalence of A. phagocytophilum in horses. For example, Slivinska et al. (2016) tested 39 horses from Slovakia by PCR and observed only one positive case

Open access

genes have been used to detect S. pneumoniae by a real-time PCR assay including genes encoding the pneumococcal surface protein A ( pspA ), the virulence factors pneumolysin ( ply ), autolysin ( lytA ), the DNA fragment Spn9802 of unknown function and

Restricted access
Acta Microbiologica et Immunologica Hungarica
Authors:
Yussef Moghaddam
,
Hajar Ziaei Hezarjaribi
,
Abdol Sattar Pagheh
,
Mahdi Fakhar
,
Reza Saberi
,
Mitra Sharbatkhori
,
Mahbobeh Montazeri
,
Hossein Ghalehnoei
, and
Eisa Nazar

inspected the parasites under high dry magnification (1000×) (Zeiss; Germany). Considering the elevated likelihood of Leishmania parasite presence in auricle and snout specimens, these samples were cultured in NNN culture medium. DNA extraction and PCR

Restricted access
Acta Microbiologica et Immunologica Hungarica
Authors:
Sara Davoudabadi
,
Hossein Goudarzi
,
Mehdi Goudarzi
,
Abdollah Ardebili
,
Ebrahim Faghihloo
,
Javad Yasbolaghi Sharahi
, and
Ali Hashemi

used. PCR detection of the virulence-associated and capsular genes According to the manufacturer’s instructions, genomic DNA from single bacterial colonies grown on primary culture plates was extracted

Restricted access

reaction (PCR) assay for the detection of the nucA gene and final confirmation [ 14 ]. Determination of susceptibility of S. aureus isolates The Kirby Bauer disk diffusion assay was used to evaluate the antibiotic susceptibility of the S. aureus

Restricted access
Acta Microbiologica et Immunologica Hungarica
Authors:
Shaghayegh Shahkolahi
,
Pegah Shakibnia
,
Shahla Shahbazi
,
Samira Sabzi
,
Farzad Badmasti
,
Mohammad Reza Asadi Karam
, and
Mehri Habibi

-lactamase genes ( bla TEM , bla SHV , bla CTX-M , and bla OXA-1 ) and class I integron ( intI1 gene) genes using the Polymerase chain reaction (PCR). For the isolates carrying integron genes, PCR amplification was also performed on variable regions. Primers

Restricted access

: lower branch of the right oculomotor nerve Moderate lymphocytic pleiocytosis was detected by cerebrospinal fluid (CSF) examination, as an indirect result of VZV infection, but VZV was not detected in the CSF by PCR (the multiplex-CSF PCR did not confirm

Open access

allantoic fluid using QIAamp Viral RNA Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instruction manual. Real-time reverse transcription polymerase chain reaction (rRT-PCR) A rRT-PCR kit (Qiagen, Inc., Valencia CA, USA), with specific

Restricted access
Acta Microbiologica et Immunologica Hungarica
Authors:
Márió Gajdács
,
Marianna Ábrók
,
Andrea Lázár
,
Laura Jánvári
,
Ákos Tóth
,
Gabriella Terhes
, and
Katalin Burián

]. Molecular methods, such as polymerase chain reaction (PCR) and whole-genome sequencing (WGS) are considered as the gold standard for detection of carbapenemase genes, nevertheless, their high laboratory costs make them unsuitable for many smaller, low

Open access

. Molecular characterization of ColR isolates by Polymerase Chain Reaction (PCR) DNA was extracted by the boiling method as previously described [ 26 ]. To detect chromosomal mutations in ColR isolates, mgrB , phoP , phoQ , pmrA , and pmrB

Restricted access