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RABV ( Badrane et al., 2001; Colombi et al., 2019 ). Many diagnostic techniques have been developed for the identification of rabies, including the fluorescent antibody test (FAT) for the detection of rabies virus antigen, RT-PCR for the detection of

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flasks of EPC monolayer at 80% confluency. The flasks were incubated at 17 °C and 20 °C and checked daily for the appearance of cytopathic effect (CPE). PCR assays For the molecular investigations, organ samples were homogenised using the TissueLyser high

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Acta Microbiologica et Immunologica Hungarica
Authors:
Zohreh Riahi Rad
,
Zahra Riahi Rad
,
Hossein Goudarzi
,
Mehdi Goudarzi
,
Hesam Alizade
,
Fariba Naeimi Mazraeh
,
Javad Yasbolaghi Sharahi
,
Abdollah Ardebili
, and
Ali Hashemi

procedure [ 18 ]. Polymerase Chain Reaction (PCR) amplification and DNA sequencing In compliance with the manufacturer's instruction, the DNA extraction kit (GeNet Bio Company, Daejeon, Korea; Cat. No, K-3000

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by centrifugation and washed with 750 µL 70% ethanol taken from −20 °C ( Liu et al., 2004 ). The quality of DNA extraction was measured by Epoch2 (BioTek, USA). Confirmation of E. coli isolates by PCR ECO-1, ECO-2 and gyrB primers specific to E

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Acta Veterinaria Hungarica
Authors:
Tábata Maués
,
Táya Figueiredo de Oliveira
,
Kênia Balbi El-Jaick
,
Agnes Marie Sá Figueiredo
,
Maria De Lourdes Gonçalves Ferreira
, and
Ana Maria Reis Ferreira

tissue for RTq-PCR calibrator was obtained from a 3-year-old female Yorkshire Terrier dog that had undergone elective spay surgery. Genomic DNA was extracted from 9 out of 21 CMC tissue samples (DNeasy Blood & Tissue Kit, Qiagen®, CA). Forward (5

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Acta Veterinaria Hungarica
Authors:
Attila Dobos
,
György Gábor
,
Enikő Wehmann
,
Béla Dénes
,
Bettina Póth-Szebenyi
,
Áron B. Kovács
, and
Miklós Gyuranecz

(9.3%) 37/96 (38.5%) Discussion The prevalence of C. burnetii infection has been reported to be 93.7% in Central and Eastern European dairy herds, and a higher prevalence of 97.2% was found in Hungary based on ELISA and PCR test findings of the bulk

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. phagocytophilum PCR-positive hosts were also examined in this study. DNA was extracted from host tissue samples and from whole ticks (individually) with the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instruction

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subsequent analysis and experimentation. Genotyping (PCR-RFLP) For the amplification of the 5′ flanking coding sequence of the IGF-1 gene, a set of primers previously described by Bakhtiar et al. (2017) was utilized in this experiment. The amplification

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-extraction of an overnight culture in Todd Hewitt and Yeast extract broth (Sigma-Aldrich Solutions, Germany). PCRs targeting genes for PI-1 and PI-2 were performed as described previously [ 8 ,  9 ]. A specific region of the rlrA gene was amplified to detect

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Acta Veterinaria Hungarica
Authors:
Federica Giorda
,
Giovanni Di Guardo
,
Katia Varello
,
Alessandra Pautasso
,
Eva Sierra
,
Maria Domenica Pintore
,
Carla Grattarola
,
Erika Molica Colella
,
Enrica Berio
,
Maria Goria
,
Elena Bozzetta
,
Cristina Casalone
, and
Barbara Iulini

chain reaction (RT-PCR) followed by sequencing the PCR product is a specific and sensitive diagnostic method; however, histology and immunohistochemistry (IHC) should be performed to confirm morbillivirus infection (MI) and to obtain information on the

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