Search Results
. pneumoniae carbapenemase) and OXA (Oxacillinase carbapenemase)-48 Confirm kit (Rosco diagnostica A/S, Denmark), according to the manufacturer's instructions. Molecular identification of ESBL and carbapenemase genes All isolates were PCR screened for the
biochemically identified with glucose fermentation, indole production, methyl red test, Voges-Proskauer, and citrate utilization standard tests. Confirmation was performed by Polymerase Chain Reaction (PCR) analysis targeting the malic acid dehydrogenase ( mdh
hospital. Bacterial identification A. baumannii was identified by conventional methods (colony observation, Gram staining and oxidase test), Api20 NE system (Biomerieux, Marcy l’Etoile, France) and PCR amplification of the intrinsic bla OXA-51 gene [ 11
. Polymerase chain reaction (PCR) PCR assays were used to amplify the pmrA , pmrB, lpxA, lpxC, lpxD , and lpsB genes [ 8 , 9 ] ( Table 1 ). This protocol has been optimized using a HotStar DNA Polymerase from Qiagen. Additional primers were designed in
bla OXA genes The bacterial whole genome was extracted by boiling method as previously described [ 17 ]. Multiplex polymerase chain reaction (M-PCR) was used for detecting of bla OXA-23-like , bla OXA-24-like and bla OXA-58-like genes [ 13
serology was positive, with IgG, IgM and IgA titers to phase I C. burnetii of 1:12,800, 0 and 1:6400, respectively, and to phase II of 1:200, 0 and 1:1600, respectively. 16S rRNA PCR from serum was negative. Serological analysis for HCV was positive but
factor, DNase, thermo-stable nuclease, as well as Gram staining. Then, all isolates were also evaluated for the presence of the sa442 gene by polymerase chain reaction (PCR) using the forward: 5′AATCTTTGTCGGTACACGATATTCTTCACG-3 and reverse: 5
), mucoviscosity-associated gene A ( magA ), putative virulence factors ( rmpA ) and Aerobactin gene were amplified by PCR using primers listed in Table 1 . The K. pneumoniae Pf/K . pneumoniae Pr1 primer pair were included in the multiplex PCR for the
-negative, estrogen receptor-positive breast cancer patients using an RT-PCR based prognostic expression signature BMC Cancer 8 339 . 83 Ueda , T , Aozasa , K , Tsujimoto , M et al. 1989
, Germany) and blood agar (Merck, Germany) for 48 h at 37 °C. A. baumannii isolates were identified by biochemical tests and confirmed using polymerase chain reaction (PCR) of the rpoB and bla OXA-51 genes and sequencing [ 1 ]. Antimicrobial
