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The aim of this study was to adapt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) (Wang et al., 2010) for determination of lactic acid bacteria cell count. Our study is helpful in developing protocols for measuring the viability of other lactic acid bacteria. We determined the cell concentration range which gives linear regression of CFU (colony forming units) and formazan production. In our experiment three authentic lactic acid bacteria strains were investigated: Lactobacillus rhamnosus VT1, L. plantarum 2142, and L. sakei DSM 2001. As data show beside the strain also the growth medium has influence on the MTT reduction activity, this means that for each special case separate calibration curve has to be prepared. Based on MTT reduction we also developed an improved microtiter plate assay which proved to be reliable for a rapid cell count determination. So our methods are only applicable for estimate cell number with fixed parameters, but under given circumstances they are fast and sensitive methods. With the modified methods we can rapidly measure the dehydrogenase enzyme activity of the lactic acid bacteria cells. With the microplate assay we can measure many conditions and strains at the same time.

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25 115 121 Marazza, J. A., Garro, M. S., de Giori, G. S. (2009) Aglycone production by Lactobacillus rhamnosus CRL981 during soymilk

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spray-dried Lactobacillus rhamnosus GG . Int. Dairy J . 15 : 399 – 409 . Anh , S.W. , de La Pena , R. , Candole , B.L. , Mew , T.W. 1986 . A new scale for rice

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Champagne, C.P., Gardner, N.J. & Lacroix, C. (2007): Fermentation technologies for the production of exopolysaccharide synthesizing Lactobacillus rhamnosus concentrated cultures. Electron J. Biotechn. , 10 , 211

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the secretion of IL-8 [ 21 ]. Zhang et al . [ 22 ] reported a decreased secretion of IL-8 by TNF-α-stimulated Caco-2 cells incubated with live and heat-inactivated Lactobacillus rhamnosus GG (LGG). However, high doses of live LGG increased the

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. Cheow , W.S. & Hadinoto , K. ( 2013 ): Biofilm-like Lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance . Biomacromolecules

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amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Appl. environ. Microbiol. , 65 , 3908–3914. Saxelin M

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Acta Alimentaria
Authors: K. Szekér, J. Beczner, A. Halász, Á. Mayer, J.M. Rezessy-Szabó, and P. Gálfi

4949 4956 Gopal, P.K., Prasad, J., Smart, J. & Gill, H.S. (2001): In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and

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, Y. & Neuhaus , F.C. (2000): Biosynthesis of lipoteichoic acid in Lactobacillus rhamnosus: role of DltD in D-alanylation. J. Bacteriol ., 182 , 2855–2864. Neuhaus F

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Acta Alimentaria
Authors: I. Němečková, H. Rohacká, K. Kučerová, Š. Tůma, P. Roubal, M. Pechačová, J. Cicvárek, and M. Plocková

Ward, L.J.H. & Timmins, M.J. (1999): Differentiation of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus by polymerase chain reaction. Lett. appl. Microbiol. , 29 , 90

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