Authors:Muhammed Enes İnanç, Şükrü Güngör, Emir Gül, Barış Atalay Uslu, and Ayhan Ata
,1′3,3′-tetramethylbenzimidazolyl-carbocyanine iodide (JC-1) [0.153 mM T3198, molecular probes, Invitrogen] with dimethyl sulphoxide (DMSO) was prepared and divided into 50-µL portions and stored at –20 °C until use.
Authors:Milan Došenović, Milena Radaković, Miloš Vučićević, Branislav Vejnović, Maja Vasiljević, Darko Marinković, and Zoran Stanimirović
cold lysis solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris, 1% Triton X–100, 10% DMSO, at pH 10) overnight at 4 °C. The slides were then placed in a horizontal gel electrophoresis chamber and submerged in freshly made cold electrophoresis buffer (pH > 13
Authors:A. Moslehi, Fatemeh Nabavizadeh, A.R. Dehpou, S.M. Tavanga, G. Hassanzadeh, A. Zekri, H. Nahrevanian, and H. Sohanaki
Endoplasmic reticulum (ER) stress provides abnormalities in insulin action, inflammatory responses, lipoprotein B100 degradation and hepatic lipogenesis. Excess accumulation of triglyceride in hepatocytes may also lead to disorders such as non-alcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Opioid peptides are involved in triglyceride and cholesterol dysregulation, inflammation and cell death. In this study, we evaluated Naltrexone effects on ER stress induced liver injury. To do so, C57/BL6 mice received saline, DMSO and Naltrexone, as control groups. ER stress was induced by tunicamycin (TM) injection. Naltrexone was given before TM administration. Liver blood flow and biochemical serum analysis were measured. Histopathological evaluations, TNF-α measurement and Real-time RT-PCR were also performed. TM challenge provokes steatosis, cellular ballooning and lobular inflammation which significantly reduced in Naltrexone treated animals. ALT, AST and TNF-α increased in the TM group and improved in the Naltrexone plus TM group. Triglyceride and cholesterol levels decreased in TM treated mice with no increase in Naltrexone treated animals. In the Naltrexone plus TM group, gene expression of Bax/Bcl-2 ratio and caspase3 significantly lowered compared with the TM group. In this study, we found that Naltrexone had a notable alleviating role in ER stress induced steatosis and liver injury.
Authors:JJ McCormick, TA VanDusseldorp, CG Ulrich, RL Lanphere, K Dokladny, PL Mosely, and CM Mermier
homogenization. PBMCs were then transferred to cell culture plates and were either treated with dimethyl sulfoxide (DMSO) vehicle (472301, Sigma-Aldrich), bafilomycin (BAF) A1 in DMSO (InvivoGen, tlrl-bafl, USA) (100 nM), or BAF and Rapa in DMSO (InvivoGen, tlrl
Authors:Aram Sharifi, Ali Ahmadi, and Abdolmajid Mohammadzadeh
planktonic S. pneumoniae . We prepared twofold serial dilutions of EOs (0.156–20 μl/ml) in TSB media, supplemented with 0.1% dimethyl sulfoxide (DMSO) to increase solubility of the EOs in the medium. Then, 100 μl of the solution was inoculated into each well
Authors:KE Nurullahoğlu-Atalık, S Kutlu, H Solak, and R Özen Koca
sulfoxide (DMSO)] were used. The concentration of DMSO in the tissue bath was always kept below 0.4%. Phenylephrine and acetylcholine were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cilostazol and atorvastatin were kindly provided by Abdi İbrahim Drug
dissolved in dimethyl sulfoxide (DMSO)] were used. The concentration of DMSO in the tissue bath was always kept below 0.4%. All drugs were obtained from Sigma (St. Louis, MO).
Authors:L Chodari, H Dariushnejad, and V Ghorbanzadeh
Life Sciences, Fremont, CA, USA), at a physiological dose of 2 mg/kg/day dissolved in dimethyl sulfoxide (DMSO) once daily for 6 weeks ( 6 ). Rats in placebo groups received DMSO vehicle with the same amount. Exercising male rats were housed