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,1′3,3′-tetramethylbenzimidazolyl-carbocyanine iodide (JC-1) [0.153 mM T3198, molecular probes, Invitrogen] with dimethyl sulphoxide (DMSO) was prepared and divided into 50-µL portions and stored at –20 °C until use. Viability of

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Acta Veterinaria Hungarica
Authors: Milan Došenović, Milena Radaković, Miloš Vučićević, Branislav Vejnović, Maja Vasiljević, Darko Marinković, and Zoran Stanimirović

cold lysis solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris, 1% Triton X–100, 10% DMSO, at pH 10) overnight at 4 °C. The slides were then placed in a horizontal gel electrophoresis chamber and submerged in freshly made cold electrophoresis buffer (pH > 13

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Acta Physiologica Hungarica
Authors: A. Moslehi, Fatemeh Nabavizadeh, A.R. Dehpou, S.M. Tavanga, G. Hassanzadeh, A. Zekri, H. Nahrevanian, and H. Sohanaki

Endoplasmic reticulum (ER) stress provides abnormalities in insulin action, inflammatory responses, lipoprotein B100 degradation and hepatic lipogenesis. Excess accumulation of triglyceride in hepatocytes may also lead to disorders such as non-alcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Opioid peptides are involved in triglyceride and cholesterol dysregulation, inflammation and cell death. In this study, we evaluated Naltrexone effects on ER stress induced liver injury. To do so, C57/BL6 mice received saline, DMSO and Naltrexone, as control groups. ER stress was induced by tunicamycin (TM) injection. Naltrexone was given before TM administration. Liver blood flow and biochemical serum analysis were measured. Histopathological evaluations, TNF-α measurement and Real-time RT-PCR were also performed. TM challenge provokes steatosis, cellular ballooning and lobular inflammation which significantly reduced in Naltrexone treated animals. ALT, AST and TNF-α increased in the TM group and improved in the Naltrexone plus TM group. Triglyceride and cholesterol levels decreased in TM treated mice with no increase in Naltrexone treated animals. In the Naltrexone plus TM group, gene expression of Bax/Bcl-2 ratio and caspase3 significantly lowered compared with the TM group. In this study, we found that Naltrexone had a notable alleviating role in ER stress induced steatosis and liver injury.

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Physiology International
Authors: JJ McCormick, TA VanDusseldorp, CG Ulrich, RL Lanphere, K Dokladny, PL Mosely, and CM Mermier

homogenization. PBMCs were then transferred to cell culture plates and were either treated with dimethyl sulfoxide (DMSO) vehicle (472301, Sigma-Aldrich), bafilomycin (BAF) A1 in DMSO (InvivoGen, tlrl-bafl, USA) (100 nM), or BAF and Rapa in DMSO (InvivoGen, tlrl

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planktonic S. pneumoniae . We prepared twofold serial dilutions of EOs (0.156–20 μl/ml) in TSB media, supplemented with 0.1% dimethyl sulfoxide (DMSO) to increase solubility of the EOs in the medium. Then, 100 μl of the solution was inoculated into each well

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Acta Veterinaria Hungarica
Authors: Erika Varga, Erzsébet Gajdócsi, Brigitta Petz Makkosné, Ildikó Salamon, and Ágnes Bali Papp

41 116 124 Bouquet, M., Selva, J. and Auroux, M. (1995): Effects of cooling and equilibration in dimethyl sulfoxide (DMSO), and cryopreservation of

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sulfoxide (DMSO)] were used. The concentration of DMSO in the tissue bath was always kept below 0.4%. Phenylephrine and acetylcholine were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cilostazol and atorvastatin were kindly provided by Abdi İbrahim Drug

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dissolved in dimethyl sulfoxide (DMSO)] were used. The concentration of DMSO in the tissue bath was always kept below 0.4%. All drugs were obtained from Sigma (St. Louis, MO). Results Verapamil-induced relaxation

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Metab Syndr Obes 2017 ; 10 : 345 – 61 . 10.2147/DMSO.S100074 2. Chawla A , Chawla R , Jaggi S . Microvascular and macrovascular complications in diabetes mellitus: distinct or continuum? Indian J Endocrinol Metab 2016 ; 20 ( 4 ): 546

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Life Sciences, Fremont, CA, USA), at a physiological dose of 2 mg/kg/day dissolved in dimethyl sulfoxide (DMSO) once daily for 6 weeks ( 6 ). Rats in placebo groups received DMSO vehicle with the same amount. Exercising male rats were housed

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