Search Results

You are looking at 11 - 20 of 32 items for :

  • "Method development and validation" x
  • Refine by Access: All Content x
Clear All

Piperine and gallic acid are of different chemical natures — piperine is an alkaloid, while gallic acid a phenolic compound. They are used as marker compounds in many plant-based formulations. A highperformance thin-layer chromatographic (HPTLC)-densitometric method has been developed and validated for the simultaneous quantification of piperine and gallic acid as such and in pharmaceutical dosage forms. Toluene-ethyl acetate (3:7) was used as mobile phase and scanning was done at 254 and 340 nm. The method was validated with respect to linearity, reproducibility, specificity, accuracy, precision, robustness, and ruggedness. Both compounds showed good linearities in the range of 250–1750 ng. LOD and LOQ for piperine were 9.98 and 33.29 ng, while for gallic acid 25 and 83.33 ng. Average % RSD values of precision for piperine and gallic acid were 0.46% and 0.72%, respectively. % Recovery was 96–103%. The method is accurate, reproducible, cost-effective, and can be used in routine analysis.

Restricted access

Various chromatographic techniques have been designed to estimate soy isoflavones in finished products as well from Glycine max (L.) Merrill. As an attempt to develop a simple, accurate, and validated method for routine analysis of daidzein and genistein, the major isoflavones as an established standardization technique, a high-performance thin-layer chromatographic (HPTLC) method has been developed. A comparative analysis of individual isoflavone (daidzein and genistein) contents in different varieties of G. max CO-2, CO (soy) 3, and local white was also carried out. Extraction efficiency of the targeted isoflavone from the seed matrix with organic solvents using ultrasonication technique was studied. The methanolic extract of the soybean seeds was taken for method development and validation procedures by HPTLC in which separation was achieved on silica gel 60 F254 HPTLC plates using chloroform-methanol (20:1 v/v) as the mobile phase. The individual isoflavones and protein contents were estimated in three different varieties of soybean in which CO-2 variety has shown the highest content.

Restricted access
JPC - Journal of Planar Chromatography - Modern TLC
Authors: Maria Gadzikowska, Anna Petruczynik, Monika Waksmundzka-Hajnos, Mirosław Hawrył, and Grzegorz Jóźwiak

M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997. Krull I.S. Analytical Method

Restricted access

M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997. Krull I.S. Analytical Method

Restricted access

. [13] S. Gurule , D. Goswami , A. Saha , Y. Modhave , A.K. Monif , Bioanalytical method development and validation using incurred samples: Quantitative estimation of

Restricted access

M.E. Swartz and I.S. Krull , Analytical Method Development and Validation, Marcel Dekker, New York, 1997, pp. 53–71. Krull I.S. Analytical

Restricted access

. [13] L.M. Gray , Analytical method development and validation of the simultaneous UV-Vis detection of fosinopril sodium, benazepril hydrochloride

Restricted access

] R. Shanmugam , K. Gowthamarajan , D.L. Priyanka , K. Madhuri , V.V. Karri Narayanareddy , Bioanalytical method development and validation for herbal quercetin in nano formulation by RPU-FLC in rabbit plasm , J

Restricted access

of 22 h. Results Method development The present investigation reported is a new RP-HPLC method development and validation of simultaneous estimation of MP, PP, and VLP. The

Open access

/band, respectively as shown in Table 4 . System suitability System suitability is an important parameter of chromatographic method development and validation. It was prominently utilized to identify the

Open access