The study of homogeneous distribution coefficients in determining the transition temperatures of isomorphologically analogous
components and in predicting the existences of some new unstable compounds has been carried out in detail with special references
to vitriols of nickel, manganese, zinc, copper and magnesium. In the course of the investigation with NiSO4·7H2O as host and54Mn as guest, the transition temperature of orthorhombic NiSO4·7H2O was shown to be 26.5 °C, and with orthorhombic ZnSO4·7H2O and MgSO4·7H2O as host and copper sulphate as guest, the limits of existences of orthorhombic CuSO4·7H2O and newly predicted CuSO4·6H2O were found to be 13.5° to 44 °C and 44° to 51 °C, respectively. In addition, the transition temperatures of orthorhombic
MnSO4·7H2O (10 °C), stable NiSO4·7H2O (30.5±5 °C) and orthorhombic ZnSO4·7H2O (39 °C) were verified. The new method of approach is very simple, reproducible and easily adaptable.
A simple, sensitive, and stability-indicating high-performance thinlayer chromatography (HPTLC)-densitometric method was developed for the quantification of biomarker naringin in the methanol extracts of stems and leaves of Rumex vesicarius. Chromatography was performed on glass-backed silica gel 60 F254 high-performance thin-layer chromatography (HPTLC) plates with ethyl acetateglacial acetic acid-MeOH-H2O (30:10:5:1, ½/½) as mobile phase. Scanning and quantification were done at 275 nm. The system was found to give compact spot for naringin at RF = 0.46 ± 0.001. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.998 with respect to area in the concentration range of 100–1000 ng. The regression equation of standard was found to be Y = 3.438X + 38.485. Naringin was subjected to acid and alkali hydrolysis, peroxide oxidation, photodegradation, dry heat, moist heat, and ultraviolet (UV) treatment. The drug undergoes complete degradation under acidic treatment and mild degradation under basic and hydrogen peroxide treatment. The degraded products were well-separated from the pure drug. The statistical analysis proves that the developed method for quantification of naringin is reproducible and selective. Due to the ability of the method in separating naringin from other constituents including its degradation products, it can be employed as stability-indicating method for in-process as well as finished products in the market. It is for the first time that authors are reporting a complete stability-indicating densitometric HPTLC method for the estimation of biomarker naringin in the leaves and stems of R. vesicarius L.
Thirty-three washing solutions with various molar concentrations were visually tested as potentially washing solutions for separation of the Western corn rootworm (WCR) eggs from the soil. The results showed that 1.5 or 2.0 molar solution of MgSO4.7H2O, 1.0 and 1.5 molar solution of ZnSO4.7H2O, 3.0, 4.0 and 5.0 molar solution of NaNO3, MgCl2.6H2Owith molarity of 2.0, NaCl with molarity of 4.0 or 5.0, Ca(NO3)2.4H2O with molarity of 1.5 and 2.0, solution of sucrose with molarity of 1.5 or 2.0, KCl with molarity of 4.0, and NaH2PO4.2H2O with molarity of 2.0, 3.0 or 4.0 were considered as suitable for the separation of WCR eggs from soil and they would be useful tool in integrated pest management for monitoring the density of WCR eggs in maize fields. After separation of the eggs from soil, the eggs were tested for their viability. Percentages of hatched eggs after separation of eggs from soil by washing solutions were not significantly different to control variant. Marginal density of washing solution was determined by beaker and tube test. Marginal density of washing solution, in which the eggs started to move up towards the surface of solution was calculated at the level of 1.086929 g/cm3. As a safe solution density for the separation of the WCR eggs from soil (the density, in which all eggs completely flew to the surface), was considered the level of 1.125 g/cm3.