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chromatography–tandem mass spectrometer (LC–MS/MS), ultra-performance LC–MS/MS (UPLC–MS/MS) has higher detection sensitivity with more powerful separation and analytical capability, which are essential for studying Chinese medicine with complex contents and the
–6 ]. Therefore, it was necessary to develop a UPLC-MS/MS method for determination of liensinine in mouse blood and its application for the pharmacokinetics. Experimental Chemicals and animals Liensinine and midazolam (IS) (both purity >98%) were obtained from
reported the pharmacokinetics of hirsutine and hirsuteine. Wu et al. [ 15 ] established an ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method to simultaneously measure rhynchophylline and hirsutine in rat plasma and also
pH using the mixture of acetonitrile and 0.1% formic acid (45:55, v / v ) as a mobile phase. The analytical run time was 3.5 min. In this paper, an ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method with an
UPLC-MS/MS method was developed for determination of calycanthine in rat plasma, and pharmacokinetics in rats was investigated. Materials and methods Chemical and animals Calycanthine (purity > 98%, Fig. 1A ) and midazolam (purity > 98%, Fig. 1B
identification, UPLC-MS/MS technology is extensively utilized because of its high sensitivity and minimal sample size requirements [ 6–10 ]. In vivo pharmacokinetic studies of fraxetin and fraxin have been reported [ 11 , 12 ], but the simultaneous
inhalation exposure of rats to opium [ 16 ]. However, the pharmacokinetics of opium was not reported. To our best knowledge, there no report of UPLC-MS/MS for simultaneous determination of these five alkaloids in plasma. In this paper, we developed a UPLC
play an important role in drug development, as they assist in predicting a variety of efficacy- and toxicity-related events. The detection sensitivity of HPLC method is low. UPLC-MS/MS technology has the advantages of high sensitivity, low detection
are only few reports on the determination of the compound by ultra-performance liquid chromatography (UPLC)–MS/MS [ 9 – 18 ]. The UPLC–MS/MS is currently considered as the best choice for supporting bioanalytical studies due to high specificity
, with the intense separation ability, excellent resolution, sensitivity, and structural characterization capabilities, the strategy of UPLC-QTOF-MS was established to capture and profile the chemical components of AMR as much as possible, while we
