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Acta Biologica Hungarica
Authors: Ahmad Hegazy, Shahira Ezzat, Iman Qasem, Mohamed Ali-Shtayeh, Mohammed Basalah, Hayssam Ali, and Ashraf Hatamleh

Somova, L. O., Nadar, A., Rammanan, P., Shode, F. O. (2003) Cardiovascular, antihyperlipidemic and antioxidant effects of oleanolic and ursolic acids in experimental hypertension. Phytomedicine 10, 115

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The essential oil of the herb Nepeta cataria L. var. citriodora was obtained by hydrodistillation in Dean-Stark apparatus and by steam distillation. The chemical composition of the oil was determined by GC-MS. Two different GC oven temperature programs were tested. The main constituents of all the samples studied were the monoterpene alcohols (R)-(+)-β-citronellol and geraniol. The largest amounts of monoterpene aldehydes (geranial and neral) were found in the hydrodistilled oil. α-Amyrin (a precursor of ursolic acid) was also detected in the oil. trans-Rose oxide, α-terpineol, geranic acid, and trace amount of rose-oxide were found in the steam distilled oil only.

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A reversed-phase high-performance liquid chromatography diode array detector (HPLC-DAD) method was developed for the separation of triterpene acids and their determination in crude extracts of Potentilla species. Symmetry octadecylsilyl silica (ODS) column with acetonitrile and water with phosphoric acid (pH = 2.5) as the mobile phase was used in the described HPLC system. The column temperature was at 20 °C, the flow rate was 0.8 mL/min. The detection wavelength was 210 nm. For quantitative purposes, the linear dependencies of analyte concentration and peak surface area were obtained with regression coefficient values 0.9983 < r 2 < 0.9995. For euscaphic acid, calibration was performed in the range of 0.07–0.525; 0.7–2.1 mg/mL, for pomolic acid in the range of 0.019–0.171; 0.209–0.665 mg/mL, for ursolic acid in the range of 0.2–1.375–3.3 mg/mL. The observed recovery of triterpene acids was from 95.3% to 103.1%. The observed values of RSD were well within the generally acceptable limit of no more than 5%. This method was successfully applied to quantify pomolic acid (PA), ursolic acid (UA) and the sum of euscaphic (EA) and tormentic acids (TA) (expressed as euscaphic acid) in roots and rhizomes of 15 various Potentilla species and 2 subspecies (i.e., P. atrosanguinea, P. recta).

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Herein, the isolation, characterization, and method validation of 5 bioactive terpenoids, viz., taraxerol (1), 3ß-hydroxyoleanane-12-one (2), betulinic acid (3), ursolic acid (4), and oleanolic acid (5) from Codonopsis ovata has been reported. A novel, accurate, and cost-effective high-performance thin-layer chromatography method was developed for the simultaneous quantification of 5 natural products on silica-gel 60 F254 plate using a ternary solvent system, n-hexane-ethyl acetate-formic acid (8.0:2.0:0.4, V/V). Markers were quantified after post-chromatographic derivatization with ceric ammonium sulfate reagent. The developed method was validated as per the International Conference on Harmonization (ICH) guidelines. All calibration curves showed a good linear relationship (r > 0.995) within the test range. Precision was evaluated by intra- and inter-day tests with relative standard deviations <1.99%, and accuracy validation recovery 85.66–91.87 with relative standard deviations >2.00°%. Oleanolic acid (5) and ursolic acid (4) were found in major quantity which is advantageous because of their anticancer, anti-inflammatory, anti-mutagenic, and anti-obesity activities. Based on our results, the developed method features good quantification parameters and can serve as an effective quality control method for standardization of Codonopsis ovata.

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Holoptelea integrifolia (Sanskrit: chirivilva, Hindi: chilbil, family: Ulmaceae) is a large deciduous tree, growing up to 18 m tall, distributed throughout the greater part of India up to an altitude of 2000 ft. It has grey bark, covered with blisters, peeling in corky scales on old trees. The plant has several medicinal properties such as antiinflammatory, anthelmintic, and antidiabetic activity. The present work was taken up with a view to lay down standards which will contribute significantly to the quality control of the medicinally useful Holoptelea species. Till date, there is no comparative phytochemical and antioxidant literature available of this traditionally valuable drug. Biomarkers such as stigmasterol, olenolic acid, ursolic acid, betulin, and lupeol were estimated through high-performance thin-layer chromatography (HPTLC) method, and it was found maximum in Behraich plant sample. The bark of Behraich’s sample shows strong antioxidant activity (2,2-diphenyl-2-picrylhydrazyl [DPPH] assay). It suggests secondary metabolites present in H. integrifolia having antioxidant effects, which indicate their effectiveness in diseases caused by overproduction of free radicals. Over all, these comparative data provide a suitable criteria to differentiate the stem barks of the above four Holoptelea barks of different places from India and open a new vista for the exploitation of these species for the development of herbal formulations.

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Shimano, T., Mizuno, M., Okamoto, H. & Adachi, K. (1956): Triterpenoids IX. A new component of Prunella , ursolic acid. Yakugaku Zasshi , 76 , 974–975 (in Japanese). Adachi K

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Acta Biologica Hungarica
Authors: W. D. Ratnasooriya, S. A. Deraniyagala, S. D. N. K. Bathige, C. L. Goonasekara, and J. R. A. C. Jayakody

. (2001) Isolation of antigenotoxic ursolic acid from Ixora coccinea flowers. Actual Biol. 23, 21-24. Isolation of antigenotoxic ursolic acid from Ixora coccinea flowers Actual Biol

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956 Balanehru, S., Nagarajan, B. (1991) Protective effect of oleanolic acid and ursolic acid against lipid peroxidation. Biochem. Inter. 24 , 981

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Clinical and Experimental Medical Journal
Authors: Saleh Alqasoumi, Mohammed Al-Dosari, Mohammed Al-Sohaibani, Tawfeq Al-Howiriny, Mohammed Al-Yahya, and Syed Rafatullah

glycyrrhetic, oleanolic and ursolic acids Il Farmaco 53 22 – 32 . [35]. M. K. Jainu V. Mohan C. S. S. Devi 2006 Gastroprotective effect of Cissus quadrangularis extract in rats with experimentally induced ulcer Indian J. Med. Res

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Acta Chromatographica
Authors: Filip Šibul, Dejan Orčić, Sanja Berežni, Goran Anačkov, and Neda Mimica-Dukić

, emodin, aloe-emodin, cis, trans –abscisic acid, isoliquiritigenin, ellagic acid, glycyrrhizin, 18 β -glycyrrhetinic acid, 4-hydroxyphenylacetic acid, formononetin, isoscopoletin, ursolic acid, and resveratrol. Plant Material and

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