Authors:Leyla Semiha Şen, Zarife Nigar Özdemir Kumral, Gülsün Memi, Feriha Ercan, Berrak C. Yeğen, and Cumhur Yeğen
endothelia of the submucosal arteries [ 2–4 ]. In contrary, application of capsaicin to afferent sensory neurons eliminates the gastric hyperemic response and mucosal damage occurs [ 5 ]. Ulcer healing accelerates when mucosal blood flow increases [ 6 ]. A
A simple, selective, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of capsaicin in the bulk drug has been developed and validated. The method employs aluminum TLC plates precoated with silica gel 60F
as stationary phase. The mobile phase was toluene-ethyl acetate 6:4 (v/v). A compact spot was obtained for capsaicin (R
0.38 ± 0.02). Densitometric analysis of capsaicin was performed in absorbance mode at 280 nm. Regression analysis of calibration data revealed a good linear relationship (r
> 0.9936) for peak-area data in the concentration range 100–1000 ng per band. The method was validated for accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. The limits of detection and quantitation were 20 and 60 ng per band, respectively. Capsaicin was subjected to acidic and alkaline hydrolysis, oxidation, and thermal degradation. The drug undergoes degradation under acidic, basic, and oxidizing conditions and at elevated temperatures. Statistical analysis proved the method enables precise, selective, and accurate analysis of capsaicin. The method can be used for identification and quantitative analysis of capsaicin in the bulk drug.
DA-5018 is a new capsaicin derivative and has analgesic effect. The objective of this work was to investigate the existence
of polymorphs and pseudopolymorphs of DA-5018 and the transformation of crystal forms. Eight crystal forms of DA-5018 have
been isolated by recrystallization and characterized by powder X-ray diffractometry (PXRD), differential scanning calorimetry
(DSC), and thermogravimetric analysis (TG).
The PXRD and DSC patterns of the eight crystal forms were different respectively.
In the dissolution studies in simulated intestinal fluid at 37±0.5°C, the solubility of Form 2 was the highest. And the solubility
in water decreased in rank order: Form 2>Form 3>Form 1>Form 5>Form 7>Form 4>Form 6>Form 8.
Eight crystal forms were shown to have a good physical stability at room temperature for 60 days.
Authors:Maria-Ioana Moise, Constantin Marutoiu, Delia Badea, Cornelia-Anca Gavrila, and Constantin Patroescu
Capsaicin was extracted from red hot pepper
fruits and from commercial red hot pepper powder and separated on silica gel 60 plates with concentration zone (Merck); toluene-acetone-chloroform, 45 + 30 + 25 (
), was used as mobile phase. Detection was performed by exposure to iodine vapor. The spots assumed to arise from capsaicin were removed from the plate and the components were extracted with chloroform. The extracts were analyzed by gas chromatography combined with mass spectrometry.
The Capsicum genus, which originates from the American continent, contains species with a chromosome number of n=12. The plants have white, lilac or purple flowers, and hollow fruit of very varied shape and size, containing glands alongside the veins that produce a pungent alkaloid known as capsaicin. The majority of varieties in the species C. annuum, grown in the largest volume throughout the world and consumed as fresh vegetables or ground spices, are non-pungent. Interspecific crosses are often possible between C. annuum and related, white-flowered species, thus facilitating breeding for resistance against various diseases and pests and the search for new, valuable traits. Species with lilac and purple flowers can be crossed with each other, but direct crosses with white-flowered species are unsuccessful.
Authors:P. Sántha, Á. Jenes, Cs. Somogyi, and Istvan Nagy
Ahluwalia J, Urban L, Bevan S, Nagy I: Anandamide regulates neuropeptide release from capsaicin-sensitive primary sensory neurons by activating both the cannabinoid 1 receptor and the vanilloid receptor 1
. Eur. J. Neurosci. 17, 2611–2618 (2003