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Acta Chromatographica
Authors:
Asit Ray
,
Biswabhusan Dash
,
Suprava Sahoo
,
Ambika Sahoo
,
Sudipta Jena
,
Basudeba Kar
,
Tuhin Chatterjee
,
Biswajit Ghosh
, and
Sanghamitra Nayak

Rhizome extracts of Hedychium coronarium are widely used as phytotherapeutics. As of date, there is no documented study on the standardization of H. coronarium extract, and the following research is an effort in this direction. Coronarin D is an important bioactive compound present in H. coronarium which shows chemopreventive activity against cancer. H. coronarium extracts were assessed for coronarin D content for the first time. The extraction was checked using different solvents: n-hexane, acetone, and methanol. Coronarin D was separated on silica gel 60F254 high-performance thin-layer chromatography (HPTLC) plates by isocratic gradient method using n-hexane-ethyl acetate (80:20 v/v) as mobile phase. Densitometric quantification was performed at 231 nm in absorption mode. This method gave a well-defined peak at R f 0.20 corresponding to coronarin D. The method was validated using International Conference on Harmonization (ICH) guidelines in terms of precision, repeatability, and accuracy. Linearity range of coronarin D was 200–1000 ng spot−1 with a correlation coefficient of R 2 ± SD = 0.9987 ± 2.62% in the concentration range of 200–1000 ng spot−1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 35 and 115 ng, respectively. Accuracy of the method was checked by recovery studies conducted at three different concentration levels, and the average percentage recovery was found to be 98.22 % for coronarin D. Among the different solvents, acetone produced maximum extraction efficiency of coronarin D. The proposed HPTLC method can be applied for robust identification and quantitative determination of coronarin D in H. coronarium extracts.

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incapacity to adopt any important new Convention. Other organizations, like the EU, UN, OECD, have taken over the lead. The centenary year of ILO is a good occasion to provide some reflections on the current ILO’s position and its future perspectives. In this

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works of other composers have been comprehensively investigated. The present study examines two orchestrations Dohnányi made in 1928, on the occasion of the Schubert Centenary. Both being virtually unknown to today's public, these are the orchestral

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unique project Nine for the Ninth Centenary (Deveta za devetstotu) stands out in particular: when the 900th anniversary of the city of Zagreb was celebrated in 1994, young members of orchestras and choirs gathered from Zagreb music schools but also from

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Abstract

We show that human subjects are sensitive to the influence of both the risks associated with investments and the influence of relatedness when deciding whether or not to invest in relatives. However, there were significant differences between subjects, with some preferring to emphasise risk while others preferred to emphasise kinship. Subjects who were better at assessing probabilities were more likely to identify risky investments, while those who were better at handling conjoint probabilities were more likely to take both kinship and risk into account.

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Abstract

Verbal and non-verbal vocal cues of co-operation and competition were assessed in 120 freely-formed dyadic conversations. In line with previous research, female/female dyads were found to be the most cooperative. However, in female/female conversations, cooperation decreased, and non-verbal competition increased, when males were present. Male/male conversation did not exhibit significant changes in style when a female audience was present. Mixed-sex conversations showed evidence of compromise between the two conversational styles; however, females were still significantly more cooperative in conversational style than males, and males exhibited higher levels of verbal competitiveness than females.

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in history as “pivotal.” However, in addition to gaining control, the one-party government established under the leadership of Mátyás Rákosi 1 also needed to establish the legitimacy of the regime. The centenary of the 1848

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Abstract

Biology and the social sciences parted company at a time when evolution itself was poorly understood. As a result, the social sciences left with a rather impoverished view of evolution, and therefore failed to take note of the developments that emerged later. Among these have been an appreciation of Tinbergen's “Four Whys”, Hamilton's broadening of the concept of fitness into what he termed “neighbour modulated fitnesses” and multi-level selection (as distinct from group selection). I argue that a better appreciation of some of these developments might go some way towards facilitating a rapprochement between the social and evolutionary sciences.

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