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Abstract  

Higher than 90% of113mIn radioactivity was bound to microaggregates. The liver uptake in mice was (80%) with low lung uptake (1.3%). With respect to99mTc-microaggregated albumin, the radiochemical yield was higher than 95%. The liver uptake in mice was about (80%) with low lung uptake (1.7%). The stability of the microaggregates was followed for two months.

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Calorimetric characterization of 2′,3′-dideoxyinosine water solution

Stability and interaction with human serum albumin

Journal of Thermal Analysis and Calorimetry
Authors: Katarzyna Michalik, Zofia Drzazga, and Anna Michnik

Abstract  

A study of 2′,3′-dideoxyinosine (ddI) stability and its interaction with human serum albumin (HSA) was carried out by differential scanning microcalorimetry DSC. Scan rate dependent and irreversible endothermic thermal degradation of ddI was analyzed with use of kinetic approach. Observed process could be interpreted in terms of simple first-order one step kinetic model. Moreover it was shown that ddI bound weakly to the human serum albumin and stabilized this protein.

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Abstract  

Kits containing macroaggregated albumin were prepared and compared with three commercial kits with respect to particle size distribution, radiochemical yield and biodistribution. Our preparation was comparable to the commercial products.

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Abstract  

The thermal denaturation process of bovine and human both fatty acid containing and fatty acid free albumins in aqueous solution was studied by use of differential scanning calorimetry. Human serum albumins were found to be more stable than their bovine counterparts. Fatty acid free albumins were characterized as generally less stable, more susceptible to aggregation, their unfolding endothermic transition was less cooperative and with the smaller degree of reversibility. Deconvolution analysis with using a non-two-state model with two component transitions showed essential differences in the thermodynamic parameters between all studied albumins, particularly regarding the high-temperature component transition.

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Orvosi Hetilap
Authors: Tamás Mándli, János Fazakas, Gábor Ther, Mónika Árkosy, Balázs Füle, Endre Németh, Judit Fazakas, Márta Hidvégi, and Szabolcs Tóth

Kubo, S., Shiomi, S., Tanaka, H. és mtsai: Evaluation of the effect of portal vein embolization on liver function by 99m-Tc-glactosyl human serum albumin scintigraphy. J. Surg. Res., 2002, 107 , 113

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Abstract  

This paper summarizes the development and preparation procedures for two particulate preparations of153Sm, namely the hydroxyapatite (HA) particles and human serum albumin (HSA) microspheres. Preparation of153Sm-labeled hydroxyapatite particles and153Sm-labeled albumin microspheres were done in two steps. Radiolabeling efficiency was greater than 95% for hydroxyapatite particles and greater than 80% for albumin microspheres.

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Abstract  

Hydroxyapatite (HA), a natural constituent of bone, was synthesized. HA particles were radiolabeled with 188Re. Radiolabeling efficiency was 95%. In vitro studies showed 5% loss of activity from particles in normal saline over a period of 2 days, whereas a dissociation rate of 9% was observed in human serum albumin.

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Abstract  

The interaction of bilirubin and99mTc-HIDA and99mTc-IODIDA has been studied in rats. The mechanism of this interaction has been examined at the level of binding with human serum albumin (HSA) by the in vitro method. Percentage of binding with HSA, and affinity constants for99mTc-IODIDA were determined with and without bilirubin. Bilirubin was labeled with99mTc and its interaction with HSA was also examined.

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Abstract  

Assay conditions for the radioimmunoassay for aflatoxin B1 using125I-radiolabel and dextran-coated charcoal for the separation of free and bound radioligand were optimized. Casein was chosen as the best protecting protein /in contrast with human serum albumin, -globulin and gelatine/. The most suitable incubation conditions are at 4°C for 18 h in darkness, radioligand sorption on the dextrancoated charcoal takes place 30 min at 4°C and the antiserum is diluted in order to reach zero specific binding in the range between 35 and 50%.

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Abstract  

Electrolytic labelling procedures have been reported for various99mTc radiopharmaceuticals which differ widely in the choice of the electrodes, working pH, applied voltage and the quantity of current passed. The authors have studied the electrolytic labelling of99mTc EHDP, gluconate and glucoheptonate with MEK extracted99mTc using tin electrodes under different experimental conditions. The results have, shown that these compounds can be efficiently labelled with99mTc in a single step procedure avoiding multiple pH adjustments. Labelling of human serum albumin microspheres suitable for lung imaging with99mTc by the electrolytic method is also reported.

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