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Barley recombinant lines obtained from crosses and backcrosses of common barley (Hordeum vulgare L.) cultivars Emir and Golden Promise with bulbosus barley grass (H. bulbosum L.) were tested against differential set of 14 Blumeria graminis D.C. Golovin ex Speer f. sp. hordei – synamorph Erysiphe graminis DC. f. sp. hordei Em Marchall isolates, pathogenic fungus causing powdery mildew. Powdery mildew resistance was found in 22 from 24 lines tested. Presence of unknown genes was postulated in 13 lines. In six of these lines the unknown genes were postulated present besides Mla12 allele originated from H. vulgare parent (cv. Emir). The only line resistant to infection, from all isolates used, was 181P94/1/3/1/1/1-2. Four other lines could be considered highly resistant, because they were susceptible to only one powdery mildew isolate. The possibilities to use the hybrid lines with identified resistance to powdery mildew, especially line 181P94/1/3/1/1/1-2 in barley breeding programs were discussed.

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): Visual selection of transgenic barley (Hordeum vulgare) structures and their regeneration into green plants . Thesis, University of Guelph. Castillo, A. M., Valles, M. P., Cistue, L. (2000): Comparison of

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Hejnák V.: 2003. The effect of drought on production of dry matter in spring barley ( Hordeum vulgare L., cv. Amulet, Krona and historical cv. Nűrnberg) — Scientia Agriculturae Bohemica vol. 34 no. 4 121

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facultative × winter growth habit barley ( Hordeum vulgare L.) mapping population. Theor. Appl. Genet. 110 :1458–1466. Bedő Z. The Vrn-H2 locus is a major determinant of flowering

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63 70 Birch, C.J. and Long, K.E. (1990). Effect of nitrogen on the growth, yield and grain protein content of barley ( Hordeum vulgare ). Australian Journal of

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, A. K. (2009): Improved in vitro technique for screening of barley ( Hordeum vulgare L.) genotypes against toxin produced by spot blotch pathogen Bipolaris sorokiniana . Indian J. of Agricultural Sciences 79, 562

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Hejnák V. (2003): The effect of drought on production of dry matter in spring barley (Hordeum vulgare L., cv. Amulet, Krona and historical cv. Nűrnberg). Scientia Agriculturae Bohemica vol. 34 no. 4 121

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288 292 Faure, S., Higgins, J., Turner, A., Laurie, D. A. (2007): The FLOWERING LOCUS T -like gene family in barley ( Hordeum vulgare L.). Genetics , 176

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Ellis, R.P. and Marshall, B. (1998). Growth, yield and grain quality of barley ( Hordeum vulgare L.) in response to nitrogen uptake. II. Plant development and rate of germination. Journal of Experimental Botany 49: 1021

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