Authors:Ireneusz Balicki and Aleksandra Sobczyńska-Rak
The objective of this study was to measure the vascular endothelial growth factor (VEGF) levels in dogs diagnosed with chronic superficial keratitis (CSK). The study was performed on 25 German shepherds (14 males and 11 females, aged between 3 and 11 years). The VEGF levels were determined in blood serum using commercially available enzyme-linked immunosorbent assay (ELISA; Quantikine Canine VEGF Immunoassay, R&D Systems). The test group of affected German shepherds was subdivided into two subgroups, based on the area of corneal neovascularisation. The first subgroup (9 patients) comprised dogs with neovascularisation observed in 1 to 2 quadrants of the right and left cornea, while the second subgroup (16 patients) comprised dogs with neovascularisation observed in 3 to 4 quadrants of the right and left cornea. The control group comprised 12 clinically healthy German shepherds (7 males and 5 females, aged between 3 and 9 years). The results were then statistically analysed by the Mann-Whitney test. The study indicated that the median serum VEGF concentration in healthy dogs was 14.9 pg/mL. The VEGF level observed in sick German shepherds was elevated (19.5 pg/mL) as compared to the values found in healthy dogs; however, a statistically significant increase in VEGF concentration, as compared to the values observed in healthy dogs, was only noted in the first subgroup, where the median VEGF concentration was 22.0 pg/mL. Elevated serum VEGF concentration was observed in German shepherds diagnosed with CSK. A statistically significant increase in VEGF levels was observed in dogs in the first stage of the disease, i.e. the early stage of neovascularisation.
Authors:Tibor Gaál, L. Wágner, F. Husvéth, H. A. Manilla, P. Vajdovich, N. Balogh, I. Lóth, and Katalin Németh S.
The influence of fish oil (highly unsaturated) and beef tallow (highly saturated) with vitamin E (100 IU/kg) supplementation on the antioxidant status of broiler chicken cockerels was investigated. Chicks were fed a control diet with no added fat, 40 g/kg each of fish oil and beef tallow diets, respectively, from 11 to 42 days of age. Tocopherol concentration and the rate of lipid peroxidation, thiobarbituric acid reactive substance (TBARS) in liver, fatty acid composition of the liver lipids, blood serum total antioxidant status (TAS), and reduced glutathione (GSH) content were determined. Vitamin E supplementation of the diet increased liver ?-tocopherol content in chicks regardless of the type of dietary fat. Fish oil diet resulted in higher liver TBARS value while beef tallow diet showed lower values compared to the control diet. Vitamin E supplementation reduced liver TBARS as well as serum GSH, and raised serum TAS for all diets. Serum GSH was the same for vitamin E supplemented diets regardless of the fat supplement. Fish oil diets resulted in a significant increase in hepatic lipid n-3 PUFA content. A significant positive correlation was found between liver TBARS and n-3 PUFA content. No relationships were established, however, between liver TBARS and n-6 PUFA or saturated fatty acids. The results suggest that feeding oils rich in n-3 PUFA increases tissue concentration of these fatty acids, consequently increasing tissue lipid peroxidation and reducing the antioxidative status of broiler chickens. Supplementing high levels of vitamin E with such oils may increase tissue oxidative stability. Serum TAS or GSH may be used as a measure of antioxidative status in chickens.
Authors:Stefania Iwańska, Danuta Strusińska, and Barbara Pysera
Huber , J. T. , Emery , R. S. , Bergen , W. G. , Liesman , J. S. , Kung , L. , Jr. , Gardner , R. W. and Checketts , M. ( 1984 ): Influences of methionine hydroxy analog on milk and milk fat production, bloodserum lipids and plasma
Authors:Hamid Arazi, Siavash Rahmati, and Hosein Ghafoori
). *Statistically significant difference compared with non-Su groups
The results of biochemical analysis of the bloodserum are listed in Table II . Neither the RT nor the administration of Su modified significantly the mean
Authors:Monica Chavez Vivas, Hector Fabio Villamarin Guerrero, Antonio Jose Tascon, and Augusto Valderrama-Aguirre
part in the systemic response to infection [ 11–17 ]. This study was aimed at assessing the levels of IL-6 in the bloodserum of patients with sepsis and septic shock. The results demonstrated significant decline of IL-6 at 48 h after the
Authors:Joana Korablioviene, Mykolas Mauricas, Irena Dumalakiene, Saulius Caplinskas, Rita Viliene, Justinas Baleisis, Gintautas Vysniauskis, Joanna Chorostowska-Wynimko, Ginreta Magelinskiene, Pavel Korabliov, and Arunas Valiulis
analyzer (Boule Medical AB, Sweden). Bloodserum from selected immunized mice was used for western blotting. After blood was collected in Eppendorf tubes, incubated for 20 min at room temperature, and centrifuged for 10 min at 2,500 rpm they were stored at
Authors:Nazaryan Rozana, Kryvenko Liudmyla, and Gargin Vitaliy
[ 17 , 29 , 34 ], and indicate that the level of VEGF correlates with the degree of severity of the atopic state. At the same time (quite unexpectedly) in the last paper, there is a lack of correlation between the severity of atopy and bloodserum
Authors:Igor Ujčič-Vrhovnik, Tanja Švara, Tadej Malovrh, and Breda Jakovac-Strajn
, 2006 ). Hence, in addition to clinical observations regarding parturition and the measurement of immunoglobulin concentrations in the colostrum and the bloodserum of piglets as published in a previous report ( Jakovac-Strajn et al., 2009 ), in this