Authors:E. Ivanišová, K. Meňhartová, M. Terentjeva, L. Godočíková, J. Árvay, and M. Kačániová
The aim of the present study was to determine the microbial composition, antioxidant activity, and content of phytochemicals in prepared kombucha tea beverage. Microbiota was identified by MALDI-TOF mass spectrometry, antioxidant activity of beverage was tested by ABTS and phosphomolybdenum method, the total content of phytochemicals (polyphenols, flavonoids, and phenolic acids) was measured by colorimetric methods. The major phenolic acids, flavonoids, and methylxanthines were detected by high performance liquid chromatography (HPLC). Candida krusei, Sphingomonas melonis, Sphingomonas aquatilis, Brevibacillus centrosporus, and Gluconobacter oxydans were the most abundant microorganisms. Antioxidant activity of kombucha tested by ABTS and phosphomolybdenum method was 1.16 mg TEAC/ml and 2.04 mg TEAC/ml, respectively, which values were higher than in black tea 0.67 and 0.81 mg TEAC/ml, respectively. Also, content of total polyphenols (0.42 mg GAE/ ml), flavonoids (0.13 mg QE/ml), and phenolic acids (0.19 mg CAE/ml) was higher in kombucha than in black tea (0.18 mg GAE/ml; 0.02 mg QE/ml; 0.05 mg CAE/ml, respectively). Gallic, chlorogenic, syringic, and protocatechuic acids, and rutin and vitexin from flavonoids were dominant in kombucha beverage detected by HPLC. Strong difference in caffeine contents, 217.81 µg ml−1 (black tea) and 100.72 µg ml−1 (kombucha beverage), was observed. The amounts of theobromine were similar in black tea and kombucha, but theophylline was detected only in black tea in trace amount (0.52 µg ml−1).
Authors:Annalena Reitz, Sven Poppert, Melanie Rieker, and Hagen Frickmann
overlap with previous reports [ 4 – 7 ]. However, the samples are not identical, and therefore, the results are not equal.
In the meantime, MALDI–TOF–MS (matrix assisted laser desorption ionization–time of flight–mass spectrometry) is the
Authors:Carola Edler, Henri Derschum, Mirko Köhler, Heinrich Neubauer, Hagen Frickmann, and Ralf Matthias Hagen
, Naumann D : Rapid identification of Burkholderia cepacia complex species including strains of the novel Taxon K, recovered from cystic fibrosis patients by intact cell MALDI-ToF mass spectrometry . Analyst 134 , 1138 – 1148 ( 2009
Authors:Hagen Frickmann, Andreas Hahn, Stefan Berlec, Johannes Ulrich, Moritz Jansson, Norbert Georg Schwarz, Philipp Warnke, and Andreas Podbielski
For the identification of the bacterial isolates assessed in this study, VITEK 2 identification cards (bioMérieux, Marcy-l’Étoile, France) or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with a Shimadzu
Authors:F. Fatehi, A. Hosseinzadeh, H. Alizadeh, and T. Brimavandi
Hordeum spontaneum (wild barley) is a good gene source to improve salt tolerance in barley because it rapidly hybridizes and recombines with barley cultivars. Proteomics can assist in identifying proteins associated with a certain environmental or developmental signal. We employed a proteomic approach to understand the mechanisms of plant responses to salinity in a salt tolerant accession of H. spontaneum. At the 4-leaf stage, wild barley plants were exposed to 0 (control treatment) or 300 mM NaCl (salt treatment). The salt treatment lasted 3 weeks. Total proteins of leaf 4 were extracted and separated by two-dimensional gel electrophoresis. More than 500 protein spots were reproducibly detected. Of these, 29 spots showed significant differences between salt treatment and control. Using MALDI-TOF-TOF MS, we identified 29 cellular proteins, which represented 16 different proteins. These were classified into six categories and a group with unknown biological function. The proteins identified were involved in many different cellular functions. Three spots were identified as unknown proteins; searching in the NCBI database revealed that there was a 71% match with clathrin assembly protein putative [Ricinus communis], a 67% match with actin binding protein [Zea mays], and a 66% match with phosphatidylinositol kinase [Arabidopsis thaliana]. Other proteins identified included ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), oxygen-evolving enhancer protein (OEE), photosystem II reaction centerWprotein (Psbw), ribosomal proteins, chloroplast RNA binding protein (ChRBP), superoxide dismutase (SOD), malate dehydrogenase (MDH), thioredoxin h (Trx), nucleoside diphosphate kinase (NDPK), profilin, translationally-controlled tumor protein (TCTP), polyamine oxidase (PAO) and universal stress protein family (USP).
Authors:Carlo Pazzini, Parviz Ahmad-Nejad, and Beniam Ghebremedhin
-assisted laser desorption ionization–time of flight (MALDI–TOF) mass spectrometry. The detection of the pathogens as ESBL-producers was determined by standard susceptibility testing and Etest [ 16 ].
2008 and 2012, phenotypic methods and VITEK 2 Compact ID/AST (bioMérieux, Marcy-l’Étoile, France) were used, while following 2012, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics, Bremen
Authors:Hans Kollenda, Hagen Frickmann, Rania Ben Helal, Dorothea Franziska Wiemer, Habiba Naija, Mohamed Sélim El Asli, Melanie Egold, Joachim Jakob Bugert, Susann Handrick, Roman Wölfel, Farouk Barguellil, and Mohamed Ben Moussa
/ionization (MALDI–TOF) mass spectroscopy (MS) analysis in a Biotyper Microflex LT mass spectrometer (Bruker Daltonik, Bremen, Germany) for re-identification of the 197 Enterobacteriaceae at the Bundeswehr Institute of Microbiology in Munich, all strains were grown