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the analyte signal at the same retention time of an LOQ sample prepared from the same matrix. Results and discussion Method development and validation The development and validation of an analytical method for quantification of nicotine in e

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included in this article, our extensive previous experience in drug product analysis and exhaustive literature searches through SciFinder® (Chemical Abstracts), ISI Web of Science, and Google Scholar were employed to guide our method development and

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condition on bench top for 48 h at room temperature. The freshly prepared sample and standard were injected by using the stored mobile phase at a time interval of 24 h. Results and Discussion Method development

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development and validation. The output signal (254 nm) was monitored and processed using QuickStart Empower Waters 2002 software. A Phenomenex Luna C-18 (150 × 4.6 mm, 5 μ) column was used for the HPLC studies and method development, as well as the

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. Narasimhan , B. M. ; Abida , K. ; Srinivas , K. Stability indicating RP-HPLC method development and validation for Oseltamivir API . Chem. Pharm. Bull. 2008 , 56 , 413 – 417 . 10.1248/cpb.56.413 24. ChabaihH , O. R ; Guermouche , S. ; Rapid

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granted the permission of the Governmental Office of Pest County, Hungary (PE/EA/385-5/2018). Biological specimens The serum of untreated rats, allocated for disposal, was used for method development and validation. Stock solutions or the aqueous working

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. Analytical method development and validation of acetaminophen, caffeine, phenylephrine hydrochloride, dextromethorphan hydrobromide and chlorpheniramine Maleate in tablet dosage form by RP- HPLC . Int. J. Pharm. Res

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, C. S. ; Panda , J. An overview of experimental designs in HPLC method development and validation . J. Pharm. Biomed. Anal. 2018 , 147 , 590 – 611 . 10.1016/j.jpba.2017.05.006 23. Unger , K. K. ; Skudas , R. ; Schulte , M. M. Particle

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