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newly emerged beta-haemolytic spirochaetes in European pig farms, the objective of the current research was to outline the occurrence of B. hampsonii in Poland for the first time, employing polymerase chain reaction (PCR) assay. The study was performed
Zoonoses Report ( European Food Safety Authority, 2022 ). PCR-based molecular assays are already available for the detection of Salmonella infection ( Aabo et al., 1993 ; Szmolka et al., 2006 ). However, these assays were developed for the detection of
mites although transmission to humans has not been proved ( Billeter et al., 2008 ). Bartonella spp. have been detected by PCR in up to 40% of Ixodes ricinus populations tested in Europe ( Dietrich et al., 2010 ). The presence of
addition of 1.0-mm zircon beads, 0.5-mm glass beads and 300 µL lysis solution. After isolation, the DNA samples were stored at –20 °C until used in PCR for molecular analysis. PCR and sequence analysis
is the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method, the test has some difficulties due to the need for specially equipped laboratory and time-consuming nature in large-scale population screenings [ 1 , 4 , 5
between January and April of 2021. During recovery, both nasopharyngeal and conjunctival swabs were taken and analyzed with real-time polymerase chain reaction (PCR) in order to show the presence of SARS-CoV-2 RNA in the sample (in the Central Laboratory
, no other obvious signs of disease or mortality were observed among the 2-year-old wels catfish. One fish with the most prominent rash was used for PCR testing for a possible viral origin of the skin disease. A pea-sized piece from the damaged skin
Microchimaerismus kimutatása nagy érzékenységű droplet digitális PCR technikával és szerepe a vérképzőőssejt-transzplantált betegek monitorozásában
Detection of microchimerism with high sensitivity droplet digital PCR technique and its significance in monitoring of hematopoietic stem cell transplanted patients
] 16 Kliman D, Castellano-Gonzalez G, Withers B, et al. Ultra-sensitive droplet digital PCR for the assessment of microchimerism in cellular therapies. Biol Blood Marrow Transplant. 2018; 24
combined PCR-RFLP method. Dis. Aquat. Org. 44, 35--39. Identification of fish parasitic Myxo-bolus (Myxosporea) species using a combined PCR-RFLP method. Dis. Aquat. Org
sensitivity of the culture method [ 6 ]. Furthermore, the use of PCR for the detection of bacterial DNA from CSF is now rapidly becoming widespread, and employing this assay can significantly increase the speed and accuracy of identification of the pathogen
